DOI: 10.1083/jcb.142.6.1501. Assembly and Function of the Actin Cytoskeleton of Yeast: Relationships between Cables and Patches

Assembly and Function of the Actin Cytoskeleton of Yeast: Relationships between Cables and Patches

10.1083/jcb.142.6.1501

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Actin in eukaryotic cells is found in different pools, with filaments being organized into a variety of supramolecular assemblies. To investigate the assembly and functional relationships between different parts of the actin cytoskeleton in one cell, we studied the morphology and dynamics of cables and patches in yeast. The fine structure of actin cables and the manner in which cables disassemble support a model in which cables are composed of a number of overlapping actin filaments. No evidence for intrinsic polarity of cables was found. To investigate to what extent different parts of the actin cytoskeleton depend on each other, we looked for relationships between cables and patches. Patches and cables were often associated, and their polarized distributions were highly correlated. Therefore, patches and cables do appear to depend on each other for assembly and function. Many cell types show rearrangements of the actin cytoskeleton, which can occur via assembly or movement of actin filaments. In our studies, dramatic changes in actin polarization did not include changes in filamentous actin. In addition, the concentration of actin patches was relatively constant as cells grew. Therefore, cells do not have bursts of activity in which new parts of the actin cytoskeleton are created.

Bibliography

Karpova, T. S., McNally, J. G., Moltz, S. L., & Cooper, J. A. (1998). Assembly and Function of the Actin Cytoskeleton of Yeast: Relationships between Cables and Patches. The Journal of Cell Biology, 142(6), 1501â1517.

Authors 4

Tatiana S. Karpova (first)
James G. McNally (additional)
Samuel L. Moltz (additional)
John A. Cooper (additional)

References 47 Referenced 103

10.1083/jcb.98.3.934 / J Cell Biol / Relationship of actin and tubulin distribution to bud growth in wild-type and morphogenetic-mutant Saccharomyces cerevisiae. by Adams (1984)
10.1083/jcb.111.1.131 / J Cell Biol / CDC42 and CDC43, two additional genes involved in budding and the establishment of cell polarity in the yeast Saccharomyces cerevisiae. by Adams (1990)
10.1091/mbc.4.5.459 / Mol Biol Cell / Unexpected combinations of null mutations in genes encoding the actin cytoskeleton are lethal in yeast by Adams (1993)
10.1038/344352a0 / Nature / Disruption of the actin cytoskeleton in yeast capping protein mutants by Amatruda (1990)
10.1083/jcb.119.5.1151 / J Cell Biol / Effects of null mutations and overexpression of capping protein on morphogenesis, actin distribution and polarized secretion in yeast by Amatruda (1992)
10.1083/jcb.137.2.399 / J Cell Biol / High rates of actin filament turnover in budding yeast and roles for actin in establishment and maintenance of cell polarity revealed using the actin inhibitor latrunculin-A by Ayscough (1997)
10.1002/jemt.1060050305 / J Electron Microsc Tech / Transmission and scanning electron microscopic examination of intracellular organelles in freeze-substituted Kloeckera and Saccharomyces cerevisiaeyeast cells by Baba (1987)
10.1242/jcs.94.2.207 / J Cell Sci / Three-dimensional analysis of morphogenesis induced by mating pheromone α factor in Saccharomyces cerevisiae. by Baba (1989)
{'key': '2023072207550346400_B9'}
10.1083/jcb.116.5.1123 / J Cell Biol / Mechanisms responsible for F-actin stabilization after lysis of polymorphonuclear leukocytes by Cano (1992)
10.1083/jcb.115.3.677 / J Cell Biol / Kinetic analysis of F-actin depolymerization in polymorphonuclear leukocyte lysates indicates that chemoattractant stimulation increases actin filament number without altering the filament length distribution by Cano (1991)
10.1083/jcb.110.4.1089 / J Cell Biol / Mechanism of the formation of contractile ring in dividing cultured animal cells. I. Recruitment of preexisting actin filaments into the cleavage furrow by Cao (1990)
10.1083/jcb.136.6.1307 / J Cell Biol / Actin depolymerizing factor (ADF/cofilin) enhances the rate of filament turnover—implication in actin-based motility by Carlier (1997)
10.1016/0014-4827(78)90520-7 / Exp Cell Res / The relationship between cell size and cell division in the yeast Saccharomyces cerevisiae. by Carter (1978)
10.1083/jcb.118.3.561 / J Cell Biol / Osmotic stress and the yeast cytoskeleton: phenotype-specific suppression of an actin mutation by Chowdhury (1992)
{'key': '2023072207550346400_B16', 'first-page': '78', 'article-title': 'Preconditioning methods for improved convergence rates in iterative reconstructions', 'volume': '12', 'author': 'Clinthorne', 'year': '1993', 'journal-title': 'IEEE (Inst Electr Electron Eng) Trans Med Imaging'} / IEEE (Inst Electr Electron Eng) Trans Med Imaging / Preconditioning methods for improved convergence rates in iterative reconstructions by Clinthorne (1993)
10.1083/jcb.108.2.495 / J Cell Biol / Reassociation of microvillar core proteins: making a microvillar core in vitro by Coluccio (1989)
10.1083/jcb.97.5.1629 / J Cell Biol / Under physiological conditions actin disassembles slowly from the nonpreferred end of an actin filament by Coluccio (1983)
{'key': '2023072207550346400_B19'}
10.1016/0014-5793(87)81513-2 / FEBS Lett / Inhibition of actin polymerization by latrunculin A by Coue (1987)
10.1073/pnas.93.9.3886 / Proc Natl Acad Sci USA / Movement of yeast cortical actin cytoskeleton visualized in vivo by Doyle (1996)
10.1083/jcb.109.5.2207 / J Cell Biol / Identification of actin nucleation activity and polymerization inhibitor in ameboid cells: their regulation by chemotactic stimulation by Hall (1989)
10.1083/jcb.118.6.1421 / J Cell Biol / Mechanisms of actin rearrangements mediating platelet activation by Hartwig (1992)
10.1016/S0022-2836(63)80008-X / J Mol Biol / Electron microscope studies on the structure of natural and synthetic protein filaments from striated muscle by Huxley (1963)
10.1083/jcb.113.3.539 / J Cell Biol / The Saccharomyces cerevisiae MYO2gene encodes an essential myosin for vectorial transport of vesicles by Johnston (1991)
{'key': '2023072207550346400_B26'}
10.1083/jcb.131.6.1483 / J Cell Biol / Actin filaments in yeast are unstable in the absence of capping protein or fimbrin by Karpova (1995)
10.1083/jcb.98.3.922 / J Cell Biol / Structural rearrangements of tubulin and actin during the cell cycle of the yeast Saccharomyces. by Kilmartin (1984)
10.1038/40418 / Nature / Cofilin promotes rapid actin filament turnover in vivo by Lappalainen (1997)
10.1083/jcb.136.3.649 / J Cell Biol / Bee1, a yeast protein with homology to Wiskott-Aldrich syndrome protein, is critical for the assembly of cortical actin cytoskeleton by Li (1997)
10.1083/jcb.125.4.825 / J Cell Biol / Immunofluorescence localization of the unconventional myosin, Myo2p, and the putative kinesin-related protein, Smy1p, to the same regions of polarized growth in Saccharomyces cerevisiae. by Lillie (1994)
10.1016/0092-8674(89)90961-6 / Cell / Disruption of the single tropomyosin gene in yeast results in the disappearance of actin cables from the cytoskeleton by Liu (1989)
10.1364/JOSAA.11.001056 / J Opt Soc Am / Artifacts in computational optical-sectioning microscopy by McNally (1994)
{'key': '2023072207550346400_B34'}
{'key': '2023072207550346400_B35'}
10.1083/jcb.120.2.421 / J Cell Biol / Cofilin is an essential component of the yeast cortical cytoskeleton by Moon (1993)
10.1038/1831751a0 / Nature / Life span of individual yeast cells by Mortimer (1959)
10.1083/jcb.125.2.381 / J Cell Biol / Ultrastructure of the yeast actin cytoskeleton and its association with the plasma membrane by Mulholland (1994)
10.1083/jcb.103.6.2747 / J Cell Biol / Rate constants for the reactions of ATP- and ADP-actin with the ends of actin filaments by Pollard (1986)
10.1364/JOSAA.9.000219 / J Opt Soc Am / Regularized linear reconstruction of three-dimensional microscopic objects from optical sections by Preza (1992)
10.1002/cm.970130302 / Cell Motil Cytoskel / Latrunculins—novel marine macrolides that disrupt microfilament organization and affect cell growth: I. Comparison with cytochalasin D by Spector (1989)
{'key': '2023072207550346400_B42'}
10.1016/0076-6879(91)94008-Z / Methods Enzymol / Assay of yeast mating reaction by Sprague (1991)
10.1016/S0955-0674(97)80156-1 / Curr Opin Cell Biol / Rho, Rac and Cdc42 GTPases regulate the organization of the actin cytoskeleton by Tapon (1997)
10.1083/jcb.132.5.861 / J Cell Biol / Movement of cortical actin patches in yeast by Waddle (1996)
10.1083/jcb.25.3.517 / J Cell Biol / The timing of deoxyribonucleic acid synthesis in the cell cycle of Saccharomyces cerevisiae. by Williamson (1965)
10.1016/S0960-9822(06)00223-5 / Curr Biol / The complex containing actin-related proteins Arp2 and Arp3 is required for the motility and integrity of yeast actin patches by Winter (1997)

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:47 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 3:55 a.m.)
Indexed	9 months, 1 week ago (Nov. 19, 2024, 10:42 a.m.)
Issued	26 years, 11 months ago (Sept. 21, 1998)
Published	26 years, 11 months ago (Sept. 21, 1998)
Published Online	26 years, 11 months ago (Sept. 21, 1998)
Published Print	26 years, 11 months ago (Sept. 21, 1998)

Funders 0

None

BibTeX

@article{Karpova_1998, title={Assembly and Function of the Actin Cytoskeleton of Yeast: Relationships between Cables and Patches}, volume={142}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.142.6.1501}, DOI={10.1083/jcb.142.6.1501}, number={6}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Karpova, Tatiana S. and McNally, James G. and Moltz, Samuel L. and Cooper, John A.}, year={1998}, month=sep, pages={1501–1517} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        11,
        19
      ]
    ],
    "date-time": "2024-11-19T15:42:10Z",
    "timestamp": 1732030930314
  },
  "reference-count": 47,
  "publisher": "Rockefeller University Press",
  "issue": "6",
  "content-domain": {
    "domain": [
      "rupress.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1998,
        9,
        21
      ]
    ]
  },
  "abstract": "<jats:p>Actin in eukaryotic cells is found in different pools, with filaments being organized into a variety of supramolecular assemblies. To investigate the assembly and functional relationships between different parts of the actin cytoskeleton in one cell, we studied the morphology and dynamics of cables and patches in yeast. The fine structure of actin cables and the manner in which cables disassemble support a model in which cables are composed of a number of overlapping actin filaments. No evidence for intrinsic polarity of cables was found.</jats:p>\n               <jats:p>To investigate to what extent different parts of the actin cytoskeleton depend on each other, we looked for relationships between cables and patches. Patches and cables were often associated, and their polarized distributions were highly correlated. Therefore, patches and cables do appear to depend on each other for assembly and function.</jats:p>\n               <jats:p>Many cell types show rearrangements of the actin cytoskeleton, which can occur via assembly or movement of actin filaments. In our studies, dramatic changes in actin polarization did not include changes in filamentous actin. In addition, the concentration of actin patches was relatively constant as cells grew. Therefore, cells do not have bursts of activity in which new parts of the actin cytoskeleton are created.</jats:p>",
  "DOI": "10.1083/jcb.142.6.1501",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2002,
        7,
        26
      ]
    ],
    "date-time": "2002-07-26T16:47:50Z",
    "timestamp": 1027702070000
  },
  "page": "1501-1517",
  "update-policy": "http://dx.doi.org/10.1083/jcb.crossmarkpolicy",
  "source": "Crossref",
  "is-referenced-by-count": 103,
  "title": "Assembly and Function of the Actin Cytoskeleton of Yeast: Relationships between Cables and Patches",
  "prefix": "10.1083",
  "volume": "142",
  "author": [
    {
      "given": "Tatiana S.",
      "family": "Karpova",
      "sequence": "first",
      "affiliation": [
        {
          "name": "*Department of Cell Biology and Physiology, \u2021Institute for Biomedical Computing, Washington University, St. Louis, Missouri 63110"
        }
      ]
    },
    {
      "given": "James G.",
      "family": "McNally",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "*Department of Cell Biology and Physiology, \u2021Institute for Biomedical Computing, Washington University, St. Louis, Missouri 63110"
        }
      ]
    },
    {
      "given": "Samuel L.",
      "family": "Moltz",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "*Department of Cell Biology and Physiology, \u2021Institute for Biomedical Computing, Washington University, St. Louis, Missouri 63110"
        }
      ]
    },
    {
      "given": "John A.",
      "family": "Cooper",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "*Department of Cell Biology and Physiology, \u2021Institute for Biomedical Computing, Washington University, St. Louis, Missouri 63110"
        }
      ]
    }
  ],
  "member": "291",
  "published-online": {
    "date-parts": [
      [
        1998,
        9,
        21
      ]
    ]
  },
  "reference": [
    {
      "key": "2023072207550346400_B1",
      "doi-asserted-by": "crossref",
      "first-page": "934",
      "DOI": "10.1083/jcb.98.3.934",
      "article-title": "Relationship of actin and tubulin distribution to bud growth in wild-type and morphogenetic-mutant Saccharomyces cerevisiae.",
      "volume": "98",
      "author": "Adams",
      "year": "1984",
      "journal-title": "J Cell Biol"
    },
    {
      "key": "2023072207550346400_B2",
      "doi-asserted-by": "crossref",
      "first-page": "131",
      "DOI": "10.1083/jcb.111.1.131",
      "article-title": "CDC42 and CDC43, two additional genes involved in budding and the establishment of cell polarity in the yeast Saccharomyces cerevisiae.",
      "volume": "111",
      "author": "Adams",
      "year": "1990",
      "journal-title": "J Cell Biol"
    },
    {
      "key": "2023072207550346400_B3",
      "doi-asserted-by": "crossref",
      "first-page": "459",
      "DOI": "10.1091/mbc.4.5.459",
      "article-title": "Unexpected combinations of null mutations in genes encoding the actin cytoskeleton are lethal in yeast",
      "volume": "4",
      "author": "Adams",
      "year": "1993",
      "journal-title": "Mol Biol Cell"
    },
    {
      "key": "2023072207550346400_B4",
      "doi-asserted-by": "crossref",
      "first-page": "352",
      "DOI": "10.1038/344352a0",
      "article-title": "Disruption of the actin cytoskeleton in yeast capping protein mutants",
      "volume": "344",
      "author": "Amatruda",
      "year": "1990",
      "journal-title": "Nature"
    },
    {
      "key": "2023072207550346400_B5",
      "doi-asserted-by": "crossref",
      "first-page": "1151",
      "DOI": "10.1083/jcb.119.5.1151",
      "article-title": "Effects of null mutations and overexpression of capping protein on morphogenesis, actin distribution and polarized secretion in yeast",
      "volume": "119",
      "author": "Amatruda",
      "year": "1992",
      "journal-title": "J Cell Biol"
    },
    {
      "key": "2023072207550346400_B6",
      "doi-asserted-by": "crossref",
      "first-page": "399",
      "DOI": "10.1083/jcb.137.2.399",
      "article-title": "High rates of actin filament turnover in budding yeast and roles for actin in establishment and maintenance of cell polarity revealed using the actin inhibitor latrunculin-A",
      "volume": "137",
      "author": "Ayscough",
      "year": "1997",
      "journal-title": "J Cell Biol"
    },
    {
      "key": "2023072207550346400_B7",
      "doi-asserted-by": "crossref",
      "first-page": "249",
      "DOI": "10.1002/jemt.1060050305",
      "article-title": "Transmission and scanning electron microscopic examination of intracellular organelles in freeze-substituted Kloeckera and Saccharomyces cerevisiaeyeast cells",
      "volume": "5",
      "author": "Baba",
      "year": "1987",
      "journal-title": "J Electron Microsc Tech"
    },
    {
      "key": "2023072207550346400_B8",
      "doi-asserted-by": "crossref",
      "first-page": "207",
      "DOI": "10.1242/jcs.94.2.207",
      "article-title": "Three-dimensional analysis of morphogenesis induced by mating pheromone \u03b1 factor in Saccharomyces cerevisiae.",
      "volume": "94",
      "author": "Baba",
      "year": "1989",
      "journal-title": "J Cell Sci"
    },
    {
      "key": "2023072207550346400_B9"
    },
    {
      "key": "2023072207550346400_B10",
      "doi-asserted-by": "crossref",
      "first-page": "1123",
      "DOI": "10.1083/jcb.116.5.1123",
      "article-title": "Mechanisms responsible for F-actin stabilization after lysis of polymorphonuclear leukocytes",
      "volume": "116",
      "author": "Cano",
      "year": "1992",
      "journal-title": "J Cell Biol"
    },
    {
      "key": "2023072207550346400_B11",
      "doi-asserted-by": "crossref",
      "first-page": "677",
      "DOI": "10.1083/jcb.115.3.677",
      "article-title": "Kinetic analysis of F-actin depolymerization in polymorphonuclear leukocyte lysates indicates that chemoattractant stimulation increases actin filament number without altering the filament length distribution",
      "volume": "115",
      "author": "Cano",
      "year": "1991",
      "journal-title": "J Cell Biol"
    },
    {
      "key": "2023072207550346400_B12",
      "doi-asserted-by": "crossref",
      "first-page": "1089",
      "DOI": "10.1083/jcb.110.4.1089",
      "article-title": "Mechanism of the formation of contractile ring in dividing cultured animal cells. I. Recruitment of preexisting actin filaments into the cleavage furrow",
      "volume": "110",
      "author": "Cao",
      "year": "1990",
      "journal-title": "J Cell Biol"
    },
    {
      "key": "2023072207550346400_B13",
      "doi-asserted-by": "crossref",
      "first-page": "1307",
      "DOI": "10.1083/jcb.136.6.1307",
      "article-title": "Actin depolymerizing factor (ADF/cofilin) enhances the rate of filament turnover\u2014implication in actin-based motility",
      "volume": "136",
      "author": "Carlier",
      "year": "1997",
      "journal-title": "J Cell Biol"
    },
    {
      "key": "2023072207550346400_B14",
      "doi-asserted-by": "crossref",
      "first-page": "15",
      "DOI": "10.1016/0014-4827(78)90520-7",
      "article-title": "The relationship between cell size and cell division in the yeast Saccharomyces cerevisiae.",
      "volume": "112",
      "author": "Carter",
      "year": "1978",
      "journal-title": "Exp Cell Res"
    },
    {
      "key": "2023072207550346400_B15",
      "doi-asserted-by": "crossref",
      "first-page": "561",
      "DOI": "10.1083/jcb.118.3.561",
      "article-title": "Osmotic stress and the yeast cytoskeleton: phenotype-specific suppression of an actin mutation",
      "volume": "118",
      "author": "Chowdhury",
      "year": "1992",
      "journal-title": "J Cell Biol"
    },
    {
      "key": "2023072207550346400_B16",
      "first-page": "78",
      "article-title": "Preconditioning methods for improved convergence rates in iterative reconstructions",
      "volume": "12",
      "author": "Clinthorne",
      "year": "1993",
      "journal-title": "IEEE (Inst Electr Electron Eng) Trans Med Imaging"
    },
    {
      "key": "2023072207550346400_B17",
      "doi-asserted-by": "crossref",
      "first-page": "495",
      "DOI": "10.1083/jcb.108.2.495",
      "article-title": "Reassociation of microvillar core proteins: making a microvillar core in vitro",
      "volume": "108",
      "author": "Coluccio",
      "year": "1989",
      "journal-title": "J Cell Biol"
    },
    {
      "key": "2023072207550346400_B18",
      "doi-asserted-by": "crossref",
      "first-page": "1629",
      "DOI": "10.1083/jcb.97.5.1629",
      "article-title": "Under physiological conditions actin disassembles slowly from the nonpreferred end of an actin filament",
      "volume": "97",
      "author": "Coluccio",
      "year": "1983",
      "journal-title": "J Cell Biol"
    },
    {
      "key": "2023072207550346400_B19"
    },
    {
      "key": "2023072207550346400_B20",
      "doi-asserted-by": "crossref",
      "first-page": "316",
      "DOI": "10.1016/0014-5793(87)81513-2",
      "article-title": "Inhibition of actin polymerization by latrunculin A",
      "volume": "213",
      "author": "Coue",
      "year": "1987",
      "journal-title": "FEBS Lett"
    },
    {
      "key": "2023072207550346400_B21",
      "doi-asserted-by": "crossref",
      "first-page": "3886",
      "DOI": "10.1073/pnas.93.9.3886",
      "article-title": "Movement of yeast cortical actin cytoskeleton visualized in vivo",
      "volume": "93",
      "author": "Doyle",
      "year": "1996",
      "journal-title": "Proc Natl Acad Sci USA"
    },
    {
      "key": "2023072207550346400_B22",
      "doi-asserted-by": "crossref",
      "first-page": "2207",
      "DOI": "10.1083/jcb.109.5.2207",
      "article-title": "Identification of actin nucleation activity and polymerization inhibitor in ameboid cells: their regulation by chemotactic stimulation",
      "volume": "109",
      "author": "Hall",
      "year": "1989",
      "journal-title": "J Cell Biol"
    },
    {
      "key": "2023072207550346400_B23",
      "doi-asserted-by": "crossref",
      "first-page": "1421",
      "DOI": "10.1083/jcb.118.6.1421",
      "article-title": "Mechanisms of actin rearrangements mediating platelet activation",
      "volume": "118",
      "author": "Hartwig",
      "year": "1992",
      "journal-title": "J Cell Biol"
    },
    {
      "key": "2023072207550346400_B24",
      "doi-asserted-by": "crossref",
      "first-page": "281",
      "DOI": "10.1016/S0022-2836(63)80008-X",
      "article-title": "Electron microscope studies on the structure of natural and synthetic protein filaments from striated muscle",
      "volume": "7",
      "author": "Huxley",
      "year": "1963",
      "journal-title": "J Mol Biol"
    },
    {
      "key": "2023072207550346400_B25",
      "doi-asserted-by": "crossref",
      "first-page": "539",
      "DOI": "10.1083/jcb.113.3.539",
      "article-title": "The Saccharomyces cerevisiae MYO2gene encodes an essential myosin for vectorial transport of vesicles",
      "volume": "113",
      "author": "Johnston",
      "year": "1991",
      "journal-title": "J Cell Biol"
    },
    {
      "key": "2023072207550346400_B26"
    },
    {
      "key": "2023072207550346400_B27",
      "doi-asserted-by": "crossref",
      "first-page": "1483",
      "DOI": "10.1083/jcb.131.6.1483",
      "article-title": "Actin filaments in yeast are unstable in the absence of capping protein or fimbrin",
      "volume": "131",
      "author": "Karpova",
      "year": "1995",
      "journal-title": "J Cell Biol"
    },
    {
      "key": "2023072207550346400_B28",
      "doi-asserted-by": "crossref",
      "first-page": "922",
      "DOI": "10.1083/jcb.98.3.922",
      "article-title": "Structural rearrangements of tubulin and actin during the cell cycle of the yeast Saccharomyces.",
      "volume": "98",
      "author": "Kilmartin",
      "year": "1984",
      "journal-title": "J Cell Biol"
    },
    {
      "key": "2023072207550346400_B29",
      "doi-asserted-by": "crossref",
      "first-page": "78",
      "DOI": "10.1038/40418",
      "article-title": "Cofilin promotes rapid actin filament turnover in vivo",
      "volume": "388",
      "author": "Lappalainen",
      "year": "1997",
      "journal-title": "Nature"
    },
    {
      "key": "2023072207550346400_B30",
      "doi-asserted-by": "crossref",
      "first-page": "649",
      "DOI": "10.1083/jcb.136.3.649",
      "article-title": "Bee1, a yeast protein with homology to Wiskott-Aldrich syndrome protein, is critical for the assembly of cortical actin cytoskeleton",
      "volume": "136",
      "author": "Li",
      "year": "1997",
      "journal-title": "J Cell Biol"
    },
    {
      "key": "2023072207550346400_B31",
      "doi-asserted-by": "crossref",
      "first-page": "825",
      "DOI": "10.1083/jcb.125.4.825",
      "article-title": "Immunofluorescence localization of the unconventional myosin, Myo2p, and the putative kinesin-related protein, Smy1p, to the same regions of polarized growth in Saccharomyces cerevisiae.",
      "volume": "125",
      "author": "Lillie",
      "year": "1994",
      "journal-title": "J Cell Biol"
    },
    {
      "key": "2023072207550346400_B32",
      "doi-asserted-by": "crossref",
      "first-page": "233",
      "DOI": "10.1016/0092-8674(89)90961-6",
      "article-title": "Disruption of the single tropomyosin gene in yeast results in the disappearance of actin cables from the cytoskeleton",
      "volume": "57",
      "author": "Liu",
      "year": "1989",
      "journal-title": "Cell"
    },
    {
      "key": "2023072207550346400_B33",
      "doi-asserted-by": "crossref",
      "first-page": "1056",
      "DOI": "10.1364/JOSAA.11.001056",
      "article-title": "Artifacts in computational optical-sectioning microscopy",
      "volume": "11",
      "author": "McNally",
      "year": "1994",
      "journal-title": "J Opt Soc Am"
    },
    {
      "key": "2023072207550346400_B34"
    },
    {
      "key": "2023072207550346400_B35"
    },
    {
      "key": "2023072207550346400_B36",
      "doi-asserted-by": "crossref",
      "first-page": "421",
      "DOI": "10.1083/jcb.120.2.421",
      "article-title": "Cofilin is an essential component of the yeast cortical cytoskeleton",
      "volume": "120",
      "author": "Moon",
      "year": "1993",
      "journal-title": "J Cell Biol"
    },
    {
      "key": "2023072207550346400_B37",
      "doi-asserted-by": "crossref",
      "first-page": "1751",
      "DOI": "10.1038/1831751a0",
      "article-title": "Life span of individual yeast cells",
      "volume": "183",
      "author": "Mortimer",
      "year": "1959",
      "journal-title": "Nature"
    },
    {
      "key": "2023072207550346400_B38",
      "doi-asserted-by": "crossref",
      "first-page": "381",
      "DOI": "10.1083/jcb.125.2.381",
      "article-title": "Ultrastructure of the yeast actin cytoskeleton and its association with the plasma membrane",
      "volume": "125",
      "author": "Mulholland",
      "year": "1994",
      "journal-title": "J Cell Biol"
    },
    {
      "key": "2023072207550346400_B39",
      "doi-asserted-by": "crossref",
      "first-page": "2747",
      "DOI": "10.1083/jcb.103.6.2747",
      "article-title": "Rate constants for the reactions of ATP- and ADP-actin with the ends of actin filaments",
      "volume": "103",
      "author": "Pollard",
      "year": "1986",
      "journal-title": "J Cell Biol"
    },
    {
      "key": "2023072207550346400_B40",
      "doi-asserted-by": "crossref",
      "first-page": "219",
      "DOI": "10.1364/JOSAA.9.000219",
      "article-title": "Regularized linear reconstruction of three-dimensional microscopic objects from optical sections",
      "volume": "9",
      "author": "Preza",
      "year": "1992",
      "journal-title": "J Opt Soc Am"
    },
    {
      "key": "2023072207550346400_B41",
      "doi-asserted-by": "crossref",
      "first-page": "127",
      "DOI": "10.1002/cm.970130302",
      "article-title": "Latrunculins\u2014novel marine macrolides that disrupt microfilament organization and affect cell growth: I. Comparison with cytochalasin D",
      "volume": "13",
      "author": "Spector",
      "year": "1989",
      "journal-title": "Cell Motil Cytoskel"
    },
    {
      "key": "2023072207550346400_B42"
    },
    {
      "key": "2023072207550346400_B43",
      "doi-asserted-by": "crossref",
      "first-page": "77",
      "DOI": "10.1016/0076-6879(91)94008-Z",
      "article-title": "Assay of yeast mating reaction",
      "volume": "194",
      "author": "Sprague",
      "year": "1991",
      "journal-title": "Methods Enzymol"
    },
    {
      "key": "2023072207550346400_B44",
      "doi-asserted-by": "crossref",
      "first-page": "86",
      "DOI": "10.1016/S0955-0674(97)80156-1",
      "article-title": "Rho, Rac and Cdc42 GTPases regulate the organization of the actin cytoskeleton",
      "volume": "9",
      "author": "Tapon",
      "year": "1997",
      "journal-title": "Curr Opin Cell Biol"
    },
    {
      "key": "2023072207550346400_B45",
      "doi-asserted-by": "crossref",
      "first-page": "861",
      "DOI": "10.1083/jcb.132.5.861",
      "article-title": "Movement of cortical actin patches in yeast",
      "volume": "132",
      "author": "Waddle",
      "year": "1996",
      "journal-title": "J Cell Biol"
    },
    {
      "key": "2023072207550346400_B46",
      "doi-asserted-by": "crossref",
      "first-page": "517",
      "DOI": "10.1083/jcb.25.3.517",
      "article-title": "The timing of deoxyribonucleic acid synthesis in the cell cycle of Saccharomyces cerevisiae.",
      "volume": "25",
      "author": "Williamson",
      "year": "1965",
      "journal-title": "J Cell Biol"
    },
    {
      "key": "2023072207550346400_B47",
      "doi-asserted-by": "crossref",
      "first-page": "519",
      "DOI": "10.1016/S0960-9822(06)00223-5",
      "article-title": "The complex containing actin-related proteins Arp2 and Arp3 is required for the motility and integrity of yeast actin patches",
      "volume": "7",
      "author": "Winter",
      "year": "1997",
      "journal-title": "Curr Biol"
    }
  ],
  "container-title": "The Journal of Cell Biology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://rupress.org/jcb/article-pdf/142/6/1501/1493421/9805121.pdf",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "syndication"
    },
    {
      "URL": "https://rupress.org/jcb/article-pdf/142/6/1501/1493421/9805121.pdf",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        7,
        22
      ]
    ],
    "date-time": "2023-07-22T07:55:58Z",
    "timestamp": 1690012558000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://rupress.org/jcb/article/142/6/1501/15868/Assembly-and-Function-of-the-Actin-Cytoskeleton-of"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1998,
        9,
        21
      ]
    ]
  },
  "references-count": 47,
  "journal-issue": {
    "issue": "6",
    "published-print": {
      "date-parts": [
        [
          1998,
          9,
          21
        ]
      ]
    }
  },
  "URL": "http://dx.doi.org/10.1083/jcb.142.6.1501",
  "relation": {},
  "ISSN": [
    "0021-9525",
    "1540-8140"
  ],
  "subject": [],
  "published": {
    "date-parts": [
      [
        1998,
        9,
        21
      ]
    ]
  }
}