DOI: 10.1083/jcb.142.4.1121. Disruption of the Talin Gene Compromises Focal Adhesion Assembly in Undifferentiated but Not Differentiated Embryonic Stem Cells

Disruption of the Talin Gene Compromises Focal Adhesion Assembly in Undifferentiated but Not Differentiated Embryonic Stem Cells

10.1083/jcb.142.4.1121

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

We have used gene disruption to isolate two talin (−/−) ES cell mutants that contain no intact talin. The undifferentiated cells (a) were unable to spread on gelatin or laminin and grew as rounded colonies, although they were able to spread on fibronectin (b) showed reduced adhesion to laminin, but not fibronectin (c) expressed much reduced levels of β1 integrin, although levels of α5 and αV were wild-type (d) were less polarized with increased membrane protrusions compared with a vinculin (−/−) ES cell mutant (e) were unable to assemble vinculin or paxillin-containing focal adhesions or actin stress fibers on fibronectin, whereas vinculin (−/−) ES cells were able to assemble talin-containing focal adhesions. Both talin (−/−) ES cell mutants formed embryoid bodies, but differentiation was restricted to two morphologically distinct cell types. Interestingly, these differentiated talin (−/−) ES cells were able to spread and form focal adhesion-like structures containing vinculin and paxillin on fibronectin. Moreover, the levels of the β1 integrin subunit were comparable to those in wild-type ES cells. We conclude that talin is essential for β1 integrin expression and focal adhesion assembly in undifferentiated ES cells, but that a subset of differentiated cells are talin independent for both characteristics.

Bibliography

Priddle, H., Hemmings, L., Monkley, S., Woods, A., Patel, B., Sutton, D., Dunn, G. A., Zicha, D., & Critchley, D. R. (1998). Disruption of the Talin Gene Compromises Focal Adhesion Assembly in Undifferentiated but Not Differentiated Embryonic Stem Cells. The Journal of Cell Biology, 142(4), 1121â1133.

Authors 9

Helen Priddle (first)
Lance Hemmings (additional)
Susan Monkley (additional)
Alison Woods (additional)
Bipin Patel (additional)
Deborah Sutton (additional)
Graham A. Dunn (additional)
Daniel Zicha (additional)
David R. Critchley (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:47 p.m.)
Deposited	2 years ago (July 28, 2023, 8 p.m.)
Indexed	1 month, 2 weeks ago (July 8, 2025, 7:29 a.m.)
Issued	27 years ago (Aug. 24, 1998)
Published	27 years ago (Aug. 24, 1998)
Published Online	27 years ago (Aug. 24, 1998)
Published Print	27 years ago (Aug. 24, 1998)

Funders 0

None

BibTeX

@article{Priddle_1998, title={Disruption of the Talin Gene Compromises Focal Adhesion Assembly in Undifferentiated but Not Differentiated Embryonic Stem Cells}, volume={142}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.142.4.1121}, DOI={10.1083/jcb.142.4.1121}, number={4}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Priddle, Helen and Hemmings, Lance and Monkley, Susan and Woods, Alison and Patel, Bipin and Sutton, Deborah and Dunn, Graham A. and Zicha, Daniel and Critchley, David R.}, year={1998}, month=aug, pages={1121–1133} }

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  "URL": "http://dx.doi.org/10.1083/jcb.142.4.1121",
  "relation": {},
  "ISSN": [
    "0021-9525",
    "1540-8140"
  ],
  "subject": [],
  "published": {
    "date-parts": [
      [
        1998,
        8,
        24
      ]
    ]
  }
}