DOI: 10.1083/jcb.142.2.319. Nopp140 Functions as a Molecular Link Between the Nucleolus and the Coiled Bodies

Nopp140 Functions as a Molecular Link Between the Nucleolus and the Coiled Bodies

10.1083/jcb.142.2.319

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Coiled bodies are small nuclear organelles that are highly enriched in small nuclear RNAs, and that have long been thought to be associated with the nucleolus. Here we use mutational analysis, transient transfections, and the yeast two-hybrid system to show that the nucleolar phosphoprotein Nopp140 functions as a molecular link between the two prominent nuclear organelles. Exogenous Nopp140 accumulated in the nucleolus rapidly, but only after a lag phase in coiled bodies, suggesting a pathway between the two organelles. The expression of partial Nopp140 constructs exerted dominant negative effects on the endogenous Nopp140 by chasing it and other antigens that were common to both organelles out of the nucleolus. The alternating positively and negatively charged repeat domain of Nopp140 was required for targeting to both organelles. In addition, partial Nopp140 constructs caused formation of novel structures in the nucleoplasm and, in the case of the conserved carboxy terminus, led to the dispersal of coiled bodies. As a final link, we identified the coiled body–specific protein p80 coilin in a yeast two-hybrid screen with Nopp140. The interaction of the two proteins was confirmed by coimmunoprecipitation. Taken together, Nopp140 appeared to shuttle between the nucleolus and the coiled bodies, and to chaperone the transport of other molecules.

Bibliography

Isaac, C., Yang, Y., & Thomas Meier, U. (1998). Nopp140 Functions as a Molecular Link Between the Nucleolus and the Coiled Bodies. The Journal of Cell Biology, 142(2), 319â329.

Authors 3

Cynthia Isaac (first)
Yunfeng Yang (additional)
U. Thomas Meier (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:47 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 3:51 a.m.)
Indexed	1 week ago (Aug. 28, 2025, 8:48 a.m.)
Issued	27 years, 1 month ago (July 27, 1998)
Published	27 years, 1 month ago (July 27, 1998)
Published Online	27 years, 1 month ago (July 27, 1998)
Published Print	27 years, 1 month ago (July 27, 1998)

Funders 0

None

BibTeX

@article{Isaac_1998, title={Nopp140 Functions as a Molecular Link Between the Nucleolus and the Coiled Bodies}, volume={142}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.142.2.319}, DOI={10.1083/jcb.142.2.319}, number={2}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Isaac, Cynthia and Yang, Yunfeng and Thomas Meier, U.}, year={1998}, month=jul, pages={319–329} }

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