DOI: 10.1083/jcb.141.6.1415. A Giant Ubiquitin-conjugating Enzyme Related to IAP Apoptosis Inhibitors

A Giant Ubiquitin-conjugating Enzyme Related to IAP Apoptosis Inhibitors

10.1083/jcb.141.6.1415

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Ubiquitin-conjugating enzymes (UBC) catalyze the covalent attachment of ubiquitin to target proteins and are distinguished by the presence of a UBC domain required for catalysis. Previously identified members of this enzyme family are small proteins and function primarily in selective proteolysis pathways. Here we describe BRUCE (BIR repeat containing ubiquitin-conjugating enzyme), a giant (528-kD) ubiquitin-conjugating enzyme from mice. BRUCE is membrane associated and localizes to the Golgi compartment and the vesicular system. Remarkably, in addition to being an active ubiquitin-conjugating enzyme, BRUCE bears a baculovirus inhibitor of apoptosis repeat (BIR) motif, which to this date has been exclusively found in apoptosis inhibitors of the IAP-related protein family. The BIR motifs of IAP proteins are indispensable for their anti–cell death activity and are thought to function through protein–protein interaction. This suggests that BRUCE may combine properties of IAP-like proteins and ubiquitin-conjugating enzymes and indicates that the family of IAP-like proteins is structurally and functionally more diverse than previously expected.

Bibliography

Hauser, H.-P., Bardroff, M., Pyrowolakis, G., & Jentsch, S. (1998). A Giant Ubiquitin-conjugating Enzyme Related to IAP Apoptosis Inhibitors. The Journal of Cell Biology, 141(6), 1415â1422.

Authors 4

Hans-Peter Hauser (first)
Michael Bardroff (additional)
George Pyrowolakis (additional)
Stefan Jentsch (additional)

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{'key': '2023072207494482200_B2'}
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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:47 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 3:50 a.m.)
Indexed	6 days, 9 hours ago (Aug. 23, 2025, 12:59 a.m.)
Issued	27 years, 2 months ago (June 15, 1998)
Published	27 years, 2 months ago (June 15, 1998)
Published Online	27 years, 2 months ago (June 15, 1998)
Published Print	27 years, 2 months ago (June 15, 1998)

Funders 0

None

BibTeX

@article{Hauser_1998, title={A Giant Ubiquitin-conjugating Enzyme Related to IAP Apoptosis Inhibitors}, volume={141}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.141.6.1415}, DOI={10.1083/jcb.141.6.1415}, number={6}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Hauser, Hans-Peter and Bardroff, Michael and Pyrowolakis, George and Jentsch, Stefan}, year={1998}, month=jun, pages={1415–1422} }

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