DOI: 10.1083/jcb.141.4.967. Analysis of the <i>Saccharomyces</i> Spindle Pole by Matrix-assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry

Analysis of the Saccharomyces Spindle Pole by Matrix-assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry

10.1083/jcb.141.4.967

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

A highly enriched spindle pole preparation was prepared from budding yeast and fractionated by SDS gel electrophoresis. Forty-five of the gel bands that appeared enriched in this fraction were analyzed by high-mass accuracy matrix-assisted laser desorption/ ionization (MALDI) peptide mass mapping combined with sequence database searching. This identified twelve of the known spindle pole components and an additional eleven gene products that had not previously been localized to the spindle pole. Immunoelectron microscopy localized eight of these components to different parts of the spindle. One of the gene products, Ndc80p, shows homology to human HEC protein (Chen, Y., D.J. Riley, P-L. Chen, and W-H. Lee. 1997. Mol. Cell Biol. 17:6049–6056) and temperature-sensitive mutants show defects in chromosome segregation. This is the first report of the identification of the components of a large cellular organelle by MALDI peptide mapping alone.

Bibliography

Wigge, P. A., Jensen, O. N., Holmes, S., SouÃ¨s, S., Mann, M., & Kilmartin, J. V. (1998). Analysis of the Saccharomyces Spindle Pole by Matrix-assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry. The Journal of Cell Biology, 141(4), 967â977.

Authors 6

Philip A. Wigge (first)
Ole N. Jensen (additional)
Simon Holmes (additional)
Sylvie Souès (additional)
Matthias Mann (additional)
John V. Kilmartin (additional)

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Dates

Type	When
Created	23 years ago (July 26, 2002, 12:47 p.m.)
Deposited	2 years ago (July 22, 2023, 3:42 a.m.)
Indexed	3 weeks, 5 days ago (July 25, 2025, 6:53 a.m.)
Issued	27 years, 3 months ago (May 18, 1998)
Published	27 years, 3 months ago (May 18, 1998)
Published Online	27 years, 3 months ago (May 18, 1998)
Published Print	27 years, 3 months ago (May 18, 1998)

Funders 0

None

BibTeX

@article{Wigge_1998, title={Analysis of the Saccharomyces Spindle Pole by Matrix-assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry}, volume={141}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.141.4.967}, DOI={10.1083/jcb.141.4.967}, number={4}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Wigge, Philip A. and Jensen, Ole N. and Holmes, Simon and Souès, Sylvie and Mann, Matthias and Kilmartin, John V.}, year={1998}, month=may, pages={967–977} }

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