DOI: 10.1083/jcb.141.3.779. The Juxtamembrane Region of the Cadherin Cytoplasmic Tail Supports Lateral Clustering, Adhesive Strengthening, and Interaction with p120ctn

The Juxtamembrane Region of the Cadherin Cytoplasmic Tail Supports Lateral Clustering, Adhesive Strengthening, and Interaction with p120ctn

10.1083/jcb.141.3.779

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Cadherin cell–cell adhesion molecules form membrane-spanning molecular complexes that couple homophilic binding by the cadherin ectodomain to the actin cytoskeleton. A fundamental issue in cadherin biology is how this complex converts the weak intrinsic binding activity of the ectodomain into strong adhesion. Recently we demonstrated that cellular cadherins cluster in a ligand-dependent fashion when cells attached to substrata coated with the adhesive ectodomain of Xenopus C-cadherin (CEC1-5). Moreover, forced clustering of the ectodomain alone significantly strengthened adhesiveness (Yap, A.S., W.M. Brieher, M. Pruschy, and B.M. Gumbiner. Curr. Biol. 7:308–315). In this study we sought to identify the determinants of the cadherin cytoplasmic tail responsible for clustering activity. A deletion mutant of C-cadherin (CT669) that retained the juxtamembrane 94–amino acid region of the cytoplasmic tail, but not the β-catenin–binding domain, clustered upon attachment to substrata coated with CEC1-5. Like wild-type C-cadherin, this clustering was ligand dependent. In contrast, mutant molecules lacking either the complete cytoplasmic tail or just the juxtamembrane region did not cluster. The juxtamembrane region was itself sufficient to induce clustering when fused to a heterologous membrane-anchored protein, albeit in a ligand-independent fashion. The CT669 cadherin mutant also displayed significant adhesive activity when tested in laminar flow detachment assays and aggregation assays. Purification of proteins binding to the juxtamembrane region revealed that the major associated protein is p120ctn. These findings identify the juxtamembrane region of the cadherin cytoplasmic tail as a functionally active region supporting cadherin clustering and adhesive strength and raise the possibility that p120ctn is involved in clustering and cell adhesion.

Bibliography

Yap, A. S., Niessen, C. M., & Gumbiner, B. M. (1998). The Juxtamembrane Region of the Cadherin Cytoplasmic Tail Supports Lateral Clustering, Adhesive Strengthening, and Interaction with p120ctn. The Journal of Cell Biology, 141(3), 779â789.

Authors 3

Alpha S. Yap (first)
Carien M. Niessen (additional)
Barry M. Gumbiner (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:47 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 3:35 a.m.)
Indexed	2 months ago (June 26, 2025, 2:24 p.m.)
Issued	27 years, 3 months ago (May 4, 1998)
Published	27 years, 3 months ago (May 4, 1998)
Published Online	27 years, 3 months ago (May 4, 1998)
Published Print	27 years, 3 months ago (May 4, 1998)

Funders 0

None

BibTeX

@article{Yap_1998, title={The Juxtamembrane Region of the Cadherin Cytoplasmic Tail Supports Lateral Clustering, Adhesive Strengthening, and Interaction with p120ctn}, volume={141}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.141.3.779}, DOI={10.1083/jcb.141.3.779}, number={3}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Yap, Alpha S. and Niessen, Carien M. and Gumbiner, Barry M.}, year={1998}, month=may, pages={779–789} }

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