DOI: 10.1083/jcb.140.6.1395. Processivity of the Motor Protein Kinesin Requires Two Heads

Processivity of the Motor Protein Kinesin Requires Two Heads

10.1083/jcb.140.6.1395

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

A single kinesin molecule can move for hundreds of steps along a microtubule without dissociating. One hypothesis to account for this processive movement is that the binding of kinesin's two heads is coordinated so that at least one head is always bound to the microtubule. To test this hypothesis, the motility of a full-length single-headed kinesin heterodimer was examined in the in vitro microtubule gliding assay. As the surface density of single-headed kinesin was lowered, there was a steep fall both in the rate at which microtubules landed and moved over the surface, and in the distance that microtubules moved, indicating that individual single-headed kinesin motors are not processive and that some four to six single-headed kinesin molecules are necessary and sufficient to move a microtubule continuously. At high ATP concentration, individual single-headed kinesin molecules detached from microtubules very slowly (at a rate less than one per second), 100-fold slower than the detachment during two-headed motility. This slow detachment directly supports a coordinated, hand-over-hand model in which the rapid detachment of one head in the dimer is contingent on the binding of the second head.

Bibliography

Hancock, W. O., & Howard, J. (1998). Processivity of the Motor Protein Kinesin Requires Two Heads. The Journal of Cell Biology, 140(6), 1395â1405.

Authors 2

William O. Hancock (first)
Jonathon Howard (additional)

References 44 Referenced 248

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{'key': '2023072207353373500_B4', 'first-page': '1105', 'article-title': 'Motor proteins 1: kinesins', 'volume': '2', 'author': 'Bloom', 'year': '1995', 'journal-title': 'Protein Profile'} / Protein Profile / Motor proteins 1: kinesins by Bloom (1995)
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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:47 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 3:36 a.m.)
Indexed	3 weeks, 3 days ago (Aug. 6, 2025, 8:02 a.m.)
Issued	27 years, 5 months ago (March 23, 1998)
Published	27 years, 5 months ago (March 23, 1998)
Published Online	27 years, 5 months ago (March 23, 1998)
Published Print	27 years, 5 months ago (March 23, 1998)

Funders 0

None

BibTeX

@article{Hancock_1998, title={Processivity of the Motor Protein Kinesin Requires Two Heads}, volume={140}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.140.6.1395}, DOI={10.1083/jcb.140.6.1395}, number={6}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Hancock, William O. and Howard, Jonathon}, year={1998}, month=mar, pages={1395–1405} }

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