DOI: 10.1083/jcb.140.5.975. Interphase Cell Cycle Dynamics of a Late-Replicating, Heterochromatic Homogeneously Staining Region: Precise Choreography of Condensation/Decondensation and Nuclear Positioning

Interphase Cell Cycle Dynamics of a Late-Replicating, Heterochromatic Homogeneously Staining Region: Precise Choreography of Condensation/Decondensation and Nuclear Positioning

10.1083/jcb.140.5.975

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Recently we described a new method for in situ localization of specific DNA sequences, based on lac operator/repressor recognition (Robinett, C.C., A. Straight, G. Li, C. Willhelm, G. Sudlow, A. Murray, and A.S. Belmont. 1996. J. Cell Biol. 135:1685–1700). We have applied this methodology to visualize the cell cycle dynamics of an ∼90 Mbp, late-replicating, heterochromatic homogeneously staining region (HSR) in CHO cells, combining immunostaining with direct in vivo observations. Between anaphase and early G1, the HSR extends approximately twofold to a linear, ∼0.3-μm-diam chromatid, and then recondenses to a compact mass adjacent to the nuclear envelope. No further changes in HSR conformation or position are seen through mid-S phase. However, HSR DNA replication is preceded by a decondensation and movement of the HSR into the nuclear interior 4–6 h into S phase. During DNA replication the HSR resolves into linear chromatids and then recondenses into a compact mass; this is followed by a third extension of the HSR during G2/ prophase. Surprisingly, compaction of the HSR is extremely high at all stages of interphase. Preliminary ultrastructural analysis of the HSR suggests at least three levels of large-scale chromatin organization above the 30-nm fiber.

Bibliography

Li, G., Sudlow, G., & Belmont, A. S. (1998). Interphase Cell Cycle Dynamics of a Late-Replicating, Heterochromatic Homogeneously Staining Region: Precise Choreography of Condensation/Decondensation and Nuclear Positioning. The Journal of Cell Biology, 140(5), 975â989.

Authors 3

Gang Li (first)
Gail Sudlow (additional)
Andrew S. Belmont (additional)

References 37 Referenced 147

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10.1007/BF00291049 / Chromosoma / Large-scale chromatin structural domains within mitotic and interphase chromosomes in vivo and in vitro by Belmont (1989)
10.1083/jcb.123.6.1671 / J Cell Biol / Lamin B distribution and association with peripheral chromatin revealed by optical sectioning and electron microscopy tomography by Belmont (1993)
10.1016/0092-8674(88)90239-5 / Cell / The metaphase scaffold is helically folded: sister chromatids have predominately opposite helical handedness by Boy de la Tour (1988)
10.1038/360674a0 / Nature / Projection domains of MAP2 and tau determine spacings between microtubules in dendrites and axons by Chen (1992)
10.1038/381529a0 / Nature / Genetic modification of heterochromatin association and nuclear organization in Drosophila. by Csink (1996)
10.1007/BF00592572 / Histochemistry / Localization of gold in biological tissue. A photochemical method for light and electron microscopy by Dancher (1981)
10.1016/S0092-8674(00)81240-4 / Cell / Perturbation of nuclear architecture by long-distance chromosome interactions by Dernburg (1996)
10.1007/BF00660315 / Chromosoma / Cell cycle dependent chromosomal movement in pre-mitotic human T-lymphocyte nuclei by Ferguson (1992)
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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:47 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 3:27 a.m.)
Indexed	1 month, 3 weeks ago (July 2, 2025, 2:45 p.m.)
Issued	27 years, 5 months ago (March 9, 1998)
Published	27 years, 5 months ago (March 9, 1998)
Published Online	27 years, 5 months ago (March 9, 1998)
Published Print	27 years, 5 months ago (March 9, 1998)

Funders 0

None

BibTeX

@article{Li_1998, title={Interphase Cell Cycle Dynamics of a Late-Replicating, Heterochromatic Homogeneously Staining Region: Precise Choreography of Condensation/Decondensation and Nuclear Positioning}, volume={140}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.140.5.975}, DOI={10.1083/jcb.140.5.975}, number={5}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Li, Gang and Sudlow, Gail and Belmont, Andrew S.}, year={1998}, month=mar, pages={975–989} }

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