DOI: 10.1083/jcb.140.5.1125. Corequirement of Specific Phosphoinositides and Small GTP-binding Protein Cdc42 in Inducing Actin Assembly in <i>Xenopus</i> Egg Extracts

Corequirement of Specific Phosphoinositides and Small GTP-binding Protein Cdc42 in Inducing Actin Assembly in Xenopus Egg Extracts

10.1083/jcb.140.5.1125

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Both phosphoinositides and small GTP-binding proteins of the Rho family have been postulated to regulate actin assembly in cells. We have reconstituted actin assembly in response to these signals in Xenopus extracts and examined the relationship of these pathways. We have found that GTPγS stimulates actin assembly in the presence of endogenous membrane vesicles in low speed extracts. These membrane vesicles are required, but can be replaced by lipid vesicles prepared from purified phospholipids containing phosphoinositides. Vesicles containing phosphatidylinositol (4,5) bisphosphate or phosphatidylinositol (3,4,5) trisphosphate can induce actin assembly even in the absence of GTPγS. RhoGDI, a guanine-nucleotide dissociation inhibitor for the Rho family, inhibits phosphoinositide-induced actin assembly, suggesting the involvement of the Rho family small G proteins. Using various dominant mutants of these G proteins, we demonstrate the requirement of Cdc42 for phosphoinositide-induced actin assembly. Our results suggest that phosphoinositides may act to facilitate GTP exchange on Cdc42, as well as to anchor Cdc42 and actin nucleation activities. Hence, both phosphoinositides and Cdc42 are required to induce actin assembly in this cell-free system.

Bibliography

Le Ma, Cantley, L. C., Janmey, P. A., & Kirschner, M. W. (1998). Corequirement of Specific Phosphoinositides and Small GTP-binding Protein Cdc42 in Inducing Actin Assembly in Xenopus Egg Extracts. The Journal of Cell Biology, 140(5), 1125â1136.

Authors 4

Le Ma (first)
Lewis C. Cantley (additional)
Paul A. Janmey (additional)
Marc W. Kirschner (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:46 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 3:40 a.m.)
Indexed	2 months, 2 weeks ago (June 10, 2025, 1:48 p.m.)
Issued	27 years, 5 months ago (March 9, 1998)
Published	27 years, 5 months ago (March 9, 1998)
Published Online	27 years, 5 months ago (March 9, 1998)
Published Print	27 years, 5 months ago (March 9, 1998)

Funders 0

None

BibTeX

@article{Le_Ma_1998, title={Corequirement of Specific Phosphoinositides and Small GTP-binding Protein Cdc42 in Inducing Actin Assembly in Xenopus Egg Extracts}, volume={140}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.140.5.1125}, DOI={10.1083/jcb.140.5.1125}, number={5}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Le Ma and Cantley, Lewis C. and Janmey, Paul A. and Kirschner, Marc W.}, year={1998}, month=mar, pages={1125–1136} }

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