DOI: 10.1083/jcb.140.5.1113. MKBP, a Novel Member of the Small Heat Shock Protein Family, Binds and Activates the Myotonic Dystrophy Protein Kinase

MKBP, a Novel Member of the Small Heat Shock Protein Family, Binds and Activates the Myotonic Dystrophy Protein Kinase

10.1083/jcb.140.5.1113

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Muscle cells are frequently subjected to severe conditions caused by heat, oxidative, and mechanical stresses. The small heat shock proteins (sHSPs) such as αB-crystallin and HSP27, which are highly expressed in muscle cells, have been suggested to play roles in maintaining myofibrillar integrity against such stresses. Here, we identified a novel member of the sHSP family that associates specifically with myotonic dystrophy protein kinase (DMPK). This DMPK-binding protein, MKBP, shows a unique nature compared with other known sHSPs: (a) In muscle cytosol, MKBP exists as an oligomeric complex separate from the complex formed by αB-crystallin and HSP27. (b) The expression of MKBP is not induced by heat shock, although it shows the characteristic early response of redistribution to the insoluble fraction like other sHSPs. Immunohistochemical analysis of skeletal muscle cells shows that MKBP localizes to the cross sections of individual myofibrils at the Z-membrane as well as the neuromuscular junction, where DMPK has been suggested to be concentrated. In vitro, MKBP enhances the kinase activity of DMPK and protects it from heat-induced inactivation. These results suggest that MKBP constitutes a novel stress-responsive system independent of other known sHSPs in muscle cells and that DMPK may be involved in this system by being activated by MKBP. Importantly, since the amount of MKBP protein, but not that of other sHSP family member proteins, is selectively upregulated in skeletal muscle from DM patients, an interaction between DMPK and MKBP may be involved in the pathogenesis of DM.

Bibliography

Suzuki, A., Sugiyama, Y., Hayashi, Y., Nyu-i, N., Yoshida, M., Nonaka, I., Ishiura, S., Arahata, K., & Ohno, S. (1998). MKBP, a Novel Member of the Small Heat Shock Protein Family, Binds and Activates the Myotonic Dystrophy Protein Kinase. The Journal of Cell Biology, 140(5), 1113â1124.

Authors 9

Atsushi Suzuki (first)
Yuki Sugiyama (additional)
Yukiko Hayashi (additional)
Nobuo Nyu-i (additional)
Michihiko Yoshida (additional)
Ikuya Nonaka (additional)
Sho-ichi Ishiura (additional)
Kiichi Arahata (additional)
Shigeo Ohno (additional)

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{'key': '2023072207410234200_B15'}
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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:47 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 3:42 a.m.)
Indexed	2 months, 1 week ago (June 19, 2025, 5:01 p.m.)
Issued	27 years, 5 months ago (March 9, 1998)
Published	27 years, 5 months ago (March 9, 1998)
Published Online	27 years, 5 months ago (March 9, 1998)
Published Print	27 years, 5 months ago (March 9, 1998)

Funders 0

None

BibTeX

@article{Suzuki_1998, title={MKBP, a Novel Member of the Small Heat Shock Protein Family, Binds and Activates the Myotonic Dystrophy Protein Kinase}, volume={140}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.140.5.1113}, DOI={10.1083/jcb.140.5.1113}, number={5}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Suzuki, Atsushi and Sugiyama, Yuki and Hayashi, Yukiko and Nyu-i, Nobuo and Yoshida, Michihiko and Nonaka, Ikuya and Ishiura, Sho-ichi and Arahata, Kiichi and Ohno, Shigeo}, year={1998}, month=mar, pages={1113–1124} }

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