DOI: 10.1083/jcb.140.4.961. CAS/Crk Coupling Serves as a “Molecular Switch” for Induction of Cell Migration

CAS/Crk Coupling Serves as a “Molecular Switch” for Induction of Cell Migration

10.1083/jcb.140.4.961

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Abstract. Carcinoma cells selected for their ability to migrate in vitro showed enhanced invasive properties in vivo. Associated with this induction of migration was the anchorage-dependent phosphorylation of p130CAS (Crk-associated substrate), leading to its coupling to the adaptor protein c-CrkII (Crk). In fact, expression of CAS or its adaptor protein partner Crk was sufficient to promote cell migration, and this depended on CAS tyrosine phosphorylation facilitating an SH2-mediated complex with Crk. Cytokine-stimulated cell migration was blocked by CAS lacking the Crk binding site or Crk containing a mutant SH2 domain. This migration response was characterized by CAS/Crk localization to membrane ruffles and blocked by the dominant-negative GTPase, Rac, but not Ras. Thus, CAS/Crk assembly serves as a “molecular switch” for the induction of cell migration and appears to contribute to the invasive property of tumors.

Bibliography

Klemke, R. L., Leng, J., Molander, R., Brooks, P. C., Vuori, K., & Cheresh, D. A. (1998). CAS/Crk Coupling Serves as a âMolecular Switchâ for Induction of Cell Migration. The Journal of Cell Biology, 140(4), 961â972.

Authors 6

Richard L. Klemke (first)
Jie Leng (additional)
Rachel Molander (additional)
Peter C. Brooks (additional)
Kristiina Vuori (additional)
David A. Cheresh (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:47 p.m.)
Deposited	2 years ago (July 28, 2023, 7:04 p.m.)
Indexed	8 hours, 26 minutes ago (Aug. 27, 2025, 12:06 p.m.)
Issued	27 years, 6 months ago (Feb. 23, 1998)
Published	27 years, 6 months ago (Feb. 23, 1998)
Published Online	27 years, 6 months ago (Feb. 23, 1998)
Published Print	27 years, 6 months ago (Feb. 23, 1998)

Funders 0

None

BibTeX

@article{Klemke_1998, title={CAS/Crk Coupling Serves as a “Molecular Switch” for Induction of Cell Migration}, volume={140}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.140.4.961}, DOI={10.1083/jcb.140.4.961}, number={4}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Klemke, Richard L. and Leng, Jie and Molander, Rachel and Brooks, Peter C. and Vuori, Kristiina and Cheresh, David A.}, year={1998}, month=feb, pages={961–972} }

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