DOI: 10.1083/jcb.140.3.461. Pheromone-regulated Genes Required for Yeast Mating Differentiation

Pheromone-regulated Genes Required for Yeast Mating Differentiation

10.1083/jcb.140.3.461

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Yeast cells mate by an inducible pathway that involves agglutination, mating projection formation, cell fusion, and nuclear fusion. To obtain insight into the mating differentiation of Saccharomyces cerevisiae, we carried out a large-scale transposon tagging screen to identify genes whose expression is regulated by mating pheromone. 91,200 transformants containing random lacZ insertions were screened for β-galactosidase (β-gal) expression in the presence and absence of α factor, and 189 strains containing pheromone-regulated lacZ insertions were identified. Transposon insertion alleles corresponding to 20 genes that are novel or had not previously been known to be pheromone regulated were examined for effects on the mating process. Mutations in four novel genes, FIG1, FIG2, KAR5/ FIG3, and FIG4 were found to cause mating defects. Three of the proteins encoded by these genes, Fig1p, Fig2p, and Fig4p, are dispensible for cell polarization in uniform concentrations of mating pheromone, but are required for normal cell polarization in mating mixtures, conditions that involve cell–cell communication. Fig1p and Fig2p are also important for cell fusion and conjugation bridge shape, respectively. The fourth protein, Kar5p/Fig3p, is required for nuclear fusion. Fig1p and Fig2p are likely to act at the cell surface as Fig1:: β-gal and Fig2::β-gal fusion proteins localize to the periphery of mating cells. Fig4p is a member of a family of eukaryotic proteins that contain a domain homologous to the yeast Sac1p. Our results indicate that a variety of novel genes are expressed specifically during mating differentiation to mediate proper cell morphogenesis, cell fusion, and other steps of the mating process.

Bibliography

Erdman, S., Lin, L., Malczynski, M., & Snyder, M. (1998). Pheromone-regulated Genes Required for Yeast Mating Differentiation. The Journal of Cell Biology, 140(3), 461â483.

Authors 4

Scott Erdman (first)
Li Lin (additional)
Michael Malczynski (additional)
Michael Snyder (additional)

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Dates

Type	When
Created	23 years ago (July 26, 2002, 12:47 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 3:27 a.m.)
Indexed	1 month ago (July 25, 2025, 5:59 a.m.)
Issued	27 years, 6 months ago (Feb. 9, 1998)
Published	27 years, 6 months ago (Feb. 9, 1998)
Published Online	27 years, 6 months ago (Feb. 9, 1998)
Published Print	27 years, 6 months ago (Feb. 9, 1998)

Funders 0

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BibTeX

@article{Erdman_1998, title={Pheromone-regulated Genes Required for Yeast Mating Differentiation}, volume={140}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.140.3.461}, DOI={10.1083/jcb.140.3.461}, number={3}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Erdman, Scott and Lin, Li and Malczynski, Michael and Snyder, Michael}, year={1998}, month=feb, pages={461–483} }

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  "abstract": "<jats:p>Yeast cells mate by an inducible pathway that involves agglutination, mating projection formation, cell fusion, and nuclear fusion. To obtain insight into the mating differentiation of Saccharomyces cerevisiae, we carried out a large-scale transposon tagging screen to identify genes whose expression is regulated by mating pheromone. 91,200 transformants containing random lacZ insertions were screened for \u03b2-galactosidase (\u03b2-gal) expression in the presence and absence of \u03b1 factor, and 189 strains containing pheromone-regulated lacZ insertions were identified. Transposon insertion alleles corresponding to 20 genes that are novel or had not previously been known to be pheromone regulated were examined for effects on the mating process. Mutations in four novel genes, FIG1, FIG2, KAR5/ FIG3, and FIG4 were found to cause mating defects. Three of the proteins encoded by these genes, Fig1p, Fig2p, and Fig4p, are dispensible for cell polarization in uniform concentrations of mating pheromone, but are required for normal cell polarization in mating mixtures, conditions that involve cell\u2013cell communication. Fig1p and Fig2p are also important for cell fusion and conjugation bridge shape, respectively. The fourth protein, Kar5p/Fig3p, is required for nuclear fusion. Fig1p and Fig2p are likely to act at the cell surface as Fig1:: \u03b2-gal and Fig2::\u03b2-gal fusion proteins localize to the periphery of mating cells. Fig4p is a member of a family of eukaryotic proteins that contain a domain homologous to the yeast Sac1p. Our results indicate that a variety of novel genes are expressed specifically during mating differentiation to mediate proper cell morphogenesis, cell fusion, and other steps of the mating process.</jats:p>",
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