DOI: 10.1083/jcb.140.2.271. Identification of a Preinitiation Step in DNA Replication That Is Independent of Origin Recognition Complex and cdc6, but Dependent on cdk2

Identification of a Preinitiation Step in DNA Replication That Is Independent of Origin Recognition Complex and cdc6, but Dependent on cdk2

10.1083/jcb.140.2.271

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Before initiation of DNA replication, origin recognition complex (ORC) proteins, cdc6, and minichromosome maintenance (MCM) proteins bind to chromatin sequentially and form preinitiation complexes. Using Xenopus laevis egg extracts, we find that after the formation of these complexes and before initiation of DNA replication, cdc6 is rapidly removed from chromatin, possibly degraded by a cdk2-activated, ubiquitin-dependent proteolytic pathway. If this displacement is inhibited, DNA replication fails to initiate. We also find that after assembly of MCM proteins into preinitiation complexes, removal of the ORC from DNA does not block the subsequent initiation of replication. Importantly, under conditions in which both ORC and cdc6 protein are absent from preinitiation complexes, DNA replication is still dependent on cdk2 activity. Therefore, the final steps in the process leading to initiation of DNA replication during S phase of the cell cycle are independent of ORC and cdc6 proteins, but dependent on cdk2 activity.

Bibliography

Hua, X. H., & Newport, J. (1998). Identification of a Preinitiation Step in DNA Replication That Is Independent of Origin Recognition Complex and cdc6, but Dependent on cdk2. The Journal of Cell Biology, 140(2), 271â281.

Authors 2

Xuequn Helen Hua (first)
John Newport (additional)

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Dates

Type	When
Created	23 years ago (July 26, 2002, 12:47 p.m.)
Deposited	2 years ago (July 28, 2023, 6:40 p.m.)
Indexed	1 day, 19 hours ago (Aug. 23, 2025, 12:59 a.m.)
Issued	27 years, 6 months ago (Jan. 26, 1998)
Published	27 years, 6 months ago (Jan. 26, 1998)
Published Online	27 years, 6 months ago (Jan. 26, 1998)
Published Print	27 years, 6 months ago (Jan. 26, 1998)

Funders 0

None

BibTeX

@article{Hua_1998, title={Identification of a Preinitiation Step in DNA Replication That Is Independent of Origin Recognition Complex and cdc6, but Dependent on cdk2}, volume={140}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.140.2.271}, DOI={10.1083/jcb.140.2.271}, number={2}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Hua, Xuequn Helen and Newport, John}, year={1998}, month=jan, pages={271–281} }

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