DOI: 10.1083/jcb.140.1.29. Prospore Membrane Formation Defines a Developmentally Regulated Branch of the Secretory Pathway in Yeast

Prospore Membrane Formation Defines a Developmentally Regulated Branch of the Secretory Pathway in Yeast

10.1083/jcb.140.1.29

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Spore formation in yeast is an unusual form of cell division in which the daughter cells are formed within the mother cell cytoplasm. This division requires the de novo synthesis of a membrane compartment, termed the prospore membrane, which engulfs the daughter nuclei. The effect of mutations in late-acting genes on sporulation was investigated. Mutation of SEC1, SEC4, or SEC8 blocked spore formation, and electron microscopic analysis of the sec4-8 mutant indicated that this inability to produce spores was caused by a failure to form the prospore membrane. The soluble NSF attachment protein 25 (SNAP-25) homologue SEC9, by contrast, was not required for sporulation. The absence of a requirement for SEC9 was shown to be due to the sporulation-specific induction of a second, previously undescribed, SNAP-25 homologue, termed SPO20. These results define a developmentally regulated branch of the secretory pathway and suggest that spore morphogenesis in yeast proceeds by the targeting and fusion of secretory vesicles to form new plasma membranes in the interior of the mother cell. Consistent with this model, the extracellular proteins Gas1p and Cts1p were localized to an internal compartment in sporulating cells. Spore formation in yeast may be a useful model for understanding secretion-driven cell division events in a variety of plant and animal systems.

Bibliography

Neiman, A. M. (1998). Prospore Membrane Formation Defines a Developmentally Regulated Branch of the Secretory Pathway in Yeast. The Journal of Cell Biology, 140(1), 29â37.

Authors 1

Aaron M. Neiman (first)

References 41 Referenced 178

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{'key': '2023072207204795800_B5'}
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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:47 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 3:21 a.m.)
Indexed	1 month, 1 week ago (July 20, 2025, 12:12 a.m.)
Issued	27 years, 7 months ago (Jan. 12, 1998)
Published	27 years, 7 months ago (Jan. 12, 1998)
Published Online	27 years, 7 months ago (Jan. 12, 1998)
Published Print	27 years, 7 months ago (Jan. 12, 1998)

Funders 0

None

BibTeX

@article{Neiman_1998, title={Prospore Membrane Formation Defines a Developmentally Regulated Branch of the Secretory Pathway in Yeast}, volume={140}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.140.1.29}, DOI={10.1083/jcb.140.1.29}, number={1}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Neiman, Aaron M.}, year={1998}, month=jan, pages={29–37} }

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