DOI: 10.1083/jcb.14.2.183. INCORPORATION OF TRITIATED CYTIDINE INTO RIBONUCLEIC ACID BY ISOLATED PEA NUCLEI

INCORPORATION OF TRITIATED CYTIDINE INTO RIBONUCLEIC ACID BY ISOLATED PEA NUCLEI

10.1083/jcb.14.2.183

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

A new method for the isolation of the nuclei from plant tissue has been devised. This method consists in passing the plant tissue through a set of spring-loaded, counter-rotating rollers and collecting the liberated nuclei in sucrose solution containing calcium ions. A semi-automatic machine which couples the rollers with a special tissue chopping device has been constructed. It has been shown that nuclei obtained in this way actively incorporate cytidine-H3 into RNA. This incorporation is increased in the presence of nucleoside triphosphates and an energy-regenerating system.

Bibliography

Rho, J. H., & Chipchase, M. I. (1962). INCORPORATION OF TRITIATED CYTIDINE INTO RIBONUCLEIC ACID BY ISOLATED PEA NUCLEI. The Journal of Cell Biology, 14(2), 183â192.

Authors 2

Joon H. Rho (first)
Margaret I. Chipchase (additional)

References 0 Referenced 37

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 1:40 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 3:13 a.m.)
Indexed	1 day, 11 hours ago (Sept. 4, 2025, 9:46 a.m.)
Issued	63 years, 1 month ago (Aug. 1, 1962)
Published	63 years, 1 month ago (Aug. 1, 1962)
Published Online	63 years, 1 month ago (Aug. 1, 1962)
Published Print	63 years, 1 month ago (Aug. 1, 1962)

Funders 0

None

BibTeX

@article{Rho_1962, title={INCORPORATION OF TRITIATED CYTIDINE INTO RIBONUCLEIC ACID BY ISOLATED PEA NUCLEI}, volume={14}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.14.2.183}, DOI={10.1083/jcb.14.2.183}, number={2}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Rho, Joon H. and Chipchase, Margaret I.}, year={1962}, month=aug, pages={183–192} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        9,
        4
      ]
    ],
    "date-time": "2025-09-04T13:46:23Z",
    "timestamp": 1756993583783
  },
  "reference-count": 0,
  "publisher": "Rockefeller University Press",
  "issue": "2",
  "content-domain": {
    "domain": [
      "rupress.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1962,
        8,
        1
      ]
    ]
  },
  "abstract": "<jats:p>A new method for the isolation of the nuclei from plant tissue has been devised. This method consists in passing the plant tissue through a set of spring-loaded, counter-rotating rollers and collecting the liberated nuclei in sucrose solution containing calcium ions. A semi-automatic machine which couples the rollers with a special tissue chopping device has been constructed. It has been shown that nuclei obtained in this way actively incorporate cytidine-H3 into RNA. This incorporation is increased in the presence of nucleoside triphosphates and an energy-regenerating system.</jats:p>",
  "DOI": "10.1083/jcb.14.2.183",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2004,
        5,
        14
      ]
    ],
    "date-time": "2004-05-14T17:40:25Z",
    "timestamp": 1084556425000
  },
  "page": "183-192",
  "update-policy": "http://dx.doi.org/10.1083/jcb.crossmarkpolicy",
  "source": "Crossref",
  "is-referenced-by-count": 37,
  "title": "INCORPORATION OF TRITIATED CYTIDINE INTO RIBONUCLEIC ACID BY ISOLATED PEA NUCLEI",
  "prefix": "10.1083",
  "volume": "14",
  "author": [
    {
      "given": "Joon H.",
      "family": "Rho",
      "sequence": "first",
      "affiliation": [
        {
          "name": "From the Division of Biology, California Institute of Technology, Pasadena"
        }
      ]
    },
    {
      "given": "Margaret I.",
      "family": "Chipchase",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "From the Division of Biology, California Institute of Technology, Pasadena"
        }
      ]
    }
  ],
  "member": "291",
  "published-online": {
    "date-parts": [
      [
        1962,
        8,
        1
      ]
    ]
  },
  "container-title": "The Journal of Cell Biology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://rupress.org/jcb/article-pdf/14/2/183/1487339/183.pdf",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "syndication"
    },
    {
      "URL": "https://rupress.org/jcb/article-pdf/14/2/183/1487339/183.pdf",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        7,
        22
      ]
    ],
    "date-time": "2023-07-22T07:13:45Z",
    "timestamp": 1690010025000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://rupress.org/jcb/article/14/2/183/15659/INCORPORATION-OF-TRITIATED-CYTIDINE-INTO"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1962,
        8,
        1
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "2",
    "published-print": {
      "date-parts": [
        [
          1962,
          8,
          1
        ]
      ]
    }
  },
  "URL": "http://dx.doi.org/10.1083/jcb.14.2.183",
  "relation": {},
  "ISSN": [
    "1540-8140",
    "0021-9525"
  ],
  "subject": [],
  "published": {
    "date-parts": [
      [
        1962,
        8,
        1
      ]
    ]
  }
}