DOI: 10.1083/jcb.139.5.1271. Stu2p: A Microtubule-Binding Protein that Is an Essential Component of the Yeast Spindle Pole Body

Stu2p: A Microtubule-Binding Protein that Is an Essential Component of the Yeast Spindle Pole Body

10.1083/jcb.139.5.1271

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Previously we isolated tub2-423, a cold-sensitive allele of the Saccharomyces cerevisiae gene encoding β-tubulin that confers a defect in mitotic spindle function. In an attempt to identify additional proteins that are important for spindle function, we screened for suppressors of the cold sensitivity of tub2-423 and obtained two alleles of a novel gene, STU2. STU2 is an essential gene and encodes a protein whose sequence is similar to proteins identified in a variety of organisms. Stu2p localizes primarily to the spindle pole body (SPB) and to a lesser extent along spindle microtubules. Localization to the SPB is not dependent on the presence of microtubules, indicating that Stu2p is an integral component of the SPB. Stu2p also binds microtubules in vitro. We have localized the microtubule-binding domain of Stu2p to a highly basic 100-amino acid region. This region contains two imperfect repeats; both repeats appear to contribute to microtubule binding to similar extents. These results suggest that Stu2p may play a role in the attachment, organization, and/or dynamics of microtubule ends at the SPB.

Bibliography

Wang, P. J., & Huffaker, T. C. (1997). Stu2p: A Microtubule-Binding Protein that Is an Essential Component of the Yeast Spindle Pole Body. The Journal of Cell Biology, 139(5), 1271â1280.

Authors 2

Peijing Jeremy Wang (first)
Tim C. Huffaker (additional)

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Dates

Type	When
Created	23 years ago (July 26, 2002, 12:46 p.m.)
Deposited	2 years ago (July 22, 2023, 3:16 a.m.)
Indexed	2 weeks, 1 day ago (Aug. 5, 2025, 8:34 a.m.)
Issued	27 years, 8 months ago (Dec. 1, 1997)
Published	27 years, 8 months ago (Dec. 1, 1997)
Published Online	27 years, 8 months ago (Dec. 1, 1997)
Published Print	27 years, 8 months ago (Dec. 1, 1997)

Funders 0

None

BibTeX

@article{Wang_1997, title={Stu2p: A Microtubule-Binding Protein that Is an Essential Component of the Yeast Spindle Pole Body}, volume={139}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.139.5.1271}, DOI={10.1083/jcb.139.5.1271}, number={5}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Wang, Peijing Jeremy and Huffaker, Tim C.}, year={1997}, month=dec, pages={1271–1280} }

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