DOI: 10.1083/jcb.139.5.1137. Golgi Tubule Traffic and the Effects of Brefeldin A Visualized in Living Cells

Golgi Tubule Traffic and the Effects of Brefeldin A Visualized in Living Cells

10.1083/jcb.139.5.1137

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

The Golgi complex is a dynamic organelle engaged in both secretory and retrograde membrane traffic. Here, we use green fluorescent protein–Golgi protein chimeras to study Golgi morphology in vivo. In untreated cells, membrane tubules were a ubiquitous, prominent feature of the Golgi complex, serving both to interconnect adjacent Golgi elements and to carry membrane outward along microtubules after detaching from stable Golgi structures. Brefeldin A treatment, which reversibly disassembles the Golgi complex, accentuated tubule formation without tubule detachment. A tubule network extending throughout the cytoplasm was quickly generated and persisted for 5–10 min until rapidly emptying Golgi contents into the ER within 15–30 s. Both lipid and protein emptied from the Golgi at similar rapid rates, leaving no Golgi structure behind, indicating that Golgi membranes do not simply mix but are absorbed into the ER in BFA-treated cells. The directionality of redistribution implied Golgi membranes are at a higher free energy state than ER membranes. Analysis of its kinetics suggested a mechanism that is analogous to wetting or adsorptive phenomena in which a tension-driven membrane flow supplements diffusive transfer of Golgi membrane into the ER. Such nonselective, flow-assisted transport of Golgi membranes into ER suggests that mechanisms that regulate retrograde tubule formation and detachment from the Golgi complex are integral to the existence and maintenance of this organelle.

Bibliography

Sciaky, N., Presley, J., Smith, C., Zaal, K. J. M., Cole, N., Moreira, J. E., Terasaki, M., Siggia, E., & Lippincott-Schwartz, J. (1997). Golgi Tubule Traffic and the Effects of Brefeldin A Visualized in Living Cells. The Journal of Cell Biology, 139(5), 1137â1155.

Authors 9

Noah Sciaky (first)
John Presley (additional)
Carolyn Smith (additional)
Kristien J.M. Zaal (additional)
Nelson Cole (additional)
Jorge E. Moreira (additional)
Mark Terasaki (additional)
Eric Siggia (additional)
Jennifer Lippincott-Schwartz (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:47 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 3:30 a.m.)
Indexed	5 days, 1 hour ago (Aug. 28, 2025, 8:38 a.m.)
Issued	27 years, 9 months ago (Dec. 1, 1997)
Published	27 years, 9 months ago (Dec. 1, 1997)
Published Online	27 years, 9 months ago (Dec. 1, 1997)
Published Print	27 years, 9 months ago (Dec. 1, 1997)

Funders 0

None

BibTeX

@article{Sciaky_1997, title={Golgi Tubule Traffic and the Effects of Brefeldin A Visualized in Living Cells}, volume={139}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.139.5.1137}, DOI={10.1083/jcb.139.5.1137}, number={5}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Sciaky, Noah and Presley, John and Smith, Carolyn and Zaal, Kristien J.M. and Cole, Nelson and Moreira, Jorge E. and Terasaki, Mark and Siggia, Eric and Lippincott-Schwartz, Jennifer}, year={1997}, month=dec, pages={1137–1155} }

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