DOI: 10.1083/jcb.138.6.1193. Nuclear Membrane Dynamics and Reassembly in Living Cells: Targeting of an Inner Nuclear Membrane Protein in Interphase and Mitosis

Nuclear Membrane Dynamics and Reassembly in Living Cells: Targeting of an Inner Nuclear Membrane Protein in Interphase and Mitosis

10.1083/jcb.138.6.1193

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

The mechanisms of localization and retention of membrane proteins in the inner nuclear membrane and the fate of this membrane system during mitosis were studied in living cells using the inner nuclear membrane protein, lamin B receptor, fused to green fluorescent protein (LBR–GFP). Photobleaching techniques revealed the majority of LBR–GFP to be completely immobilized in the nuclear envelope (NE) of interphase cells, suggesting a tight binding to heterochromatin and/or lamins. A subpopulation of LBR–GFP within ER membranes, by contrast, was entirely mobile and diffused rapidly and freely (D = 0.41 ± 0.1 μm2/s). High resolution confocal time-lapse imaging in mitotic cells revealed LBR–GFP redistributing into the interconnected ER membrane system in prometaphase, exhibiting the same high mobility and diffusion constant as observed in interphase ER membranes. LBR–GFP rapidly diffused across the cell within the membrane network defined by the ER, suggesting the integrity of the ER was maintained in mitosis, with little or no fragmentation and vesiculation. At the end of mitosis, nuclear membrane reformation coincided with immobilization of LBR–GFP in ER elements at contact sites with chromatin. LBR–GFP–containing ER membranes then wrapped around chromatin over the course of 2–3 min, quickly and efficiently compartmentalizing nuclear material. Expansion of the NE followed over the course of 30–80 min. Thus, selective changes in lateral mobility of LBR–GFP within the ER/NE membrane system form the basis for its localization to the inner nuclear membrane during interphase. Such changes, rather than vesiculation mechanisms, also underlie the redistribution of this molecule during NE disassembly and reformation in mitosis.

Bibliography

Ellenberg, J., Siggia, E. D., Moreira, J. E., Smith, C. L., Presley, J. F., Worman, H. J., & Lippincott-Schwartz, J. (1997). Nuclear Membrane Dynamics and Reassembly in Living Cells: Targeting of an Inner Nuclear Membrane Protein in Interphase and Mitosis. The Journal of Cell Biology, 138(6), 1193â1206.

Authors 7

Jan Ellenberg (first)
Eric D. Siggia (additional)
Jorge E. Moreira (additional)
Carolyn L. Smith (additional)
John F. Presley (additional)
Howard J. Worman (additional)
Jennifer Lippincott-Schwartz (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:47 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 3:10 a.m.)
Indexed	6 days, 12 hours ago (Aug. 20, 2025, 9:13 a.m.)
Issued	27 years, 11 months ago (Sept. 22, 1997)
Published	27 years, 11 months ago (Sept. 22, 1997)
Published Online	27 years, 11 months ago (Sept. 22, 1997)
Published Print	27 years, 11 months ago (Sept. 22, 1997)

Funders 0

None

BibTeX

@article{Ellenberg_1997, title={Nuclear Membrane Dynamics and Reassembly in Living Cells: Targeting of an Inner Nuclear Membrane Protein in Interphase and Mitosis}, volume={138}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.138.6.1193}, DOI={10.1083/jcb.138.6.1193}, number={6}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Ellenberg, Jan and Siggia, Eric D. and Moreira, Jorge E. and Smith, Carolyn L. and Presley, John F. and Worman, Howard J. and Lippincott-Schwartz, Jennifer}, year={1997}, month=sep, pages={1193–1206} }

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