DOI: 10.1083/jcb.138.5.1023. Kinesin-related <i>KIP3</i> of <i>Saccharomyces cerevisiae</i> Is Required for a Distinct Step in Nuclear Migration [Penn]

10.1083/jcb.138.5.1023

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Spindle orientation and nuclear migration are crucial events in cell growth and differentiation of many eukaryotes. Here we show that KIP3, the sixth and final kinesin-related gene in Saccharomyces cerevisiae, is required for migration of the nucleus to the bud site in preparation for mitosis. The position of the nucleus in the cell and the orientation of the mitotic spindle was examined by microscopy of fixed cells and by time-lapse microscopy of individual live cells. Mutations in KIP3 and in the dynein heavy chain gene defined two distinct phases of nuclear migration: a KIP3-dependent movement of the nucleus toward the incipient bud site and a dynein-dependent translocation of the nucleus through the bud neck during anaphase. Loss of KIP3 function disrupts the unidirectional movement of the nucleus toward the bud and mitotic spindle orientation, causing large oscillations in nuclear position. The oscillatory motions sometimes brought the nucleus in close proximity to the bud neck, possibly accounting for the viability of a kip3 null mutant. The kip3 null mutant exhibits normal translocation of the nucleus through the neck and normal spindle pole separation kinetics during anaphase. Simultaneous loss of KIP3 and kinesin-related KAR3 function, or of KIP3 and dynein function, is lethal but does not block any additional detectable movement. This suggests that the lethality is due to the combination of sequential and possibly overlapping defects. Epitope-tagged Kip3p localizes to astral and central spindle microtubules and is also present throughout the cytoplasm and nucleus.

Bibliography

DeZwaan, T. M., Ellingson, E., Pellman, D., & Roof, D. M. (1997). Kinesin-related KIP3 of Saccharomyces cerevisiae Is Required for a Distinct Step in Nuclear Migration. The Journal of Cell Biology, 138(5), 1023â1040.

Authors 4

Todd M. DeZwaan (first)
Eric Ellingson (additional)
David Pellman (additional)
David M. Roof (additional)

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Dates

Type	When
Created	23 years ago (July 26, 2002, 12:47 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 3:16 a.m.)
Indexed	4 months, 1 week ago (April 10, 2025, 4:27 p.m.)
Issued	27 years, 11 months ago (Sept. 8, 1997)
Published	27 years, 11 months ago (Sept. 8, 1997)
Published Online	27 years, 11 months ago (Sept. 8, 1997)
Published Print	27 years, 11 months ago (Sept. 8, 1997)

Funders 0

None

BibTeX

@article{DeZwaan_1997, title={Kinesin-related KIP3 of Saccharomyces cerevisiae Is Required for a Distinct Step in Nuclear Migration}, volume={138}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.138.5.1023}, DOI={10.1083/jcb.138.5.1023}, number={5}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={DeZwaan, Todd M. and Ellingson, Eric and Pellman, David and Roof, David M.}, year={1997}, month=sep, pages={1023–1040} }

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