DOI: 10.1083/jcb.138.4.821. Tubulin Subunits Exist in an Activated Conformational State Generated and Maintained by Protein Cofactors

Tubulin Subunits Exist in an Activated Conformational State Generated and Maintained by Protein Cofactors

10.1083/jcb.138.4.821

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

The production of native α/β tubulin heterodimer in vitro depends on the action of cytosolic chaperonin and several protein cofactors. We previously showed that four such cofactors (termed A, C, D, and E) together with native tubulin act on β-tubulin folding intermediates generated by the chaperonin to produce polymerizable tubulin heterodimers. However, this set of cofactors generates native heterodimers only very inefficiently from α-tubulin folding intermediates produced by the same chaperonin. Here we describe the isolation, characterization, and genetic analysis of a novel tubulin folding cofactor (cofactor B) that greatly enhances the efficiency of α-tubulin folding in vitro. This enabled an integrated study of α- and β-tubulin folding: we find that the pathways leading to the formation of native α- and β-tubulin converge in that the folding of the α subunit requires the participation of cofactor complexes containing the β subunit and vice versa. We also show that sequestration of native α-or β-tubulins by complex formation with cofactors results in the destabilization and decay of the remaining free subunit. These data demonstrate that tubulin folding cofactors function by placing and/or maintaining α-and β-tubulin polypeptides in an activated conformational state required for the formation of native α/β heterodimers, and imply that each subunit provides information necessary for the proper folding of the other.

Bibliography

Tian, G., Lewis, S. A., Feierbach, B., Stearns, T., Rommelaere, H., Ampe, C., & Cowan, N. J. (1997). Tubulin Subunits Exist in an Activated Conformational State Generated and Maintained by Protein Cofactors. The Journal of Cell Biology, 138(4), 821â832.

Authors 7

Guoling Tian (first)
Sally A. Lewis (additional)
Becket Feierbach (additional)
Timothy Stearns (additional)
Heidi Rommelaere (additional)
Christophe Ampe (additional)
Nicholas J. Cowan (additional)

References 38 Referenced 160

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:47 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 3:06 a.m.)
Indexed	3 weeks, 3 days ago (Aug. 7, 2025, 5:24 p.m.)
Issued	28 years ago (Aug. 25, 1997)
Published	28 years ago (Aug. 25, 1997)
Published Online	28 years ago (Aug. 25, 1997)
Published Print	28 years ago (Aug. 25, 1997)

Funders 0

None

BibTeX

@article{Tian_1997, title={Tubulin Subunits Exist in an Activated Conformational State Generated and Maintained by Protein Cofactors}, volume={138}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.138.4.821}, DOI={10.1083/jcb.138.4.821}, number={4}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Tian, Guoling and Lewis, Sally A. and Feierbach, Becket and Stearns, Timothy and Rommelaere, Heidi and Ampe, Christophe and Cowan, Nicholas J.}, year={1997}, month=aug, pages={821–832} }

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