DOI: 10.1083/jcb.138.2.291. Myosin II Is Involved in the Production of Constitutive Transport Vesicles from the TGN

Myosin II Is Involved in the Production of Constitutive Transport Vesicles from the TGN

10.1083/jcb.138.2.291

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

The participation of nonmuscle myosins in the transport of organelles and vesicular carriers along actin filaments has been documented. In contrast, there is no evidence for the involvement of myosins in the production of vesicles involved in membrane traffic. Here we show that the putative TGN coat protein p200 (Narula, N., I. McMorrow, G. Plopper, J. Doherty, K.S. Matlin, B. Burke, and J.L. Stow. 1992. J. Cell Biol. 114: 1113–1124) is myosin II. The recruitment of myosin II to Golgi membranes is dependent on actin and is regulated by G proteins. Using an assay that studies the release of transport vesicles from the TGN in vitro, we provide functional evidence that p200/myosin is involved in the assembly of basolateral transport vesicles carrying vesicular stomatitis virus G protein (VSVG) from the TGN of polarized MDCK cells. The 50% reduced efficiency in VSVG vesicle release from the TGN in vitro after depletion of p200/myosin II could be reestablished to control levels by the addition of purified nonmuscle myosin II. Several inhibitors of the actin-stimulated ATPase activity of myosin specifically inhibited the release of VSVG-containing vesicles from the TGN.

Bibliography

MÃ¼sch, A., Cohen, D., & Rodriguez-Boulan, E. (1997). Myosin II Is Involved in the Production of Constitutive Transport Vesicles from the TGN. The Journal of Cell Biology, 138(2), 291â306.

Authors 3

Anne Müsch (first)
David Cohen (additional)
Enrique Rodriguez-Boulan (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:46 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 3:08 a.m.)
Indexed	4 months ago (April 22, 2025, 3:28 a.m.)
Issued	28 years, 1 month ago (July 28, 1997)
Published	28 years, 1 month ago (July 28, 1997)
Published Online	28 years, 1 month ago (July 28, 1997)
Published Print	28 years, 1 month ago (July 28, 1997)

Funders 0

None

BibTeX

@article{M_sch_1997, title={Myosin II Is Involved in the Production of Constitutive Transport Vesicles from the TGN}, volume={138}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.138.2.291}, DOI={10.1083/jcb.138.2.291}, number={2}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Müsch, Anne and Cohen, David and Rodriguez-Boulan, Enrique}, year={1997}, month=jul, pages={291–306} }

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