DOI: 10.1083/jcb.138.1.37. Aminopeptidase I Is Targeted to the Vacuole by a Nonclassical Vesicular Mechanism

Aminopeptidase I Is Targeted to the Vacuole by a Nonclassical Vesicular Mechanism

10.1083/jcb.138.1.37

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

The yeast vacuolar protein aminopeptidase I (API) is synthesized as a cytosolic precursor that is transported to the vacuole by a nonclassical targeting mechanism. Recent genetic studies indicate that the biosynthetic pathway that transports API uses many of the same molecular components as the degradative autophagy pathway. This overlap coupled with both in vitro and in vivo analysis of API import suggested that, like autophagy, API transport is vesicular. Subcellular fractionation experiments demonstrate that API precursor (prAPI) initially enters a nonvacuolar cytosolic compartment. In addition, subvacuolar vesicles containing prAPI were purified from a mutant strain defective in breakdown of autophagosomes, further indicating that prAPI enters the vacuole inside a vesicle. The purified subvacuolar vesicles do not appear to contain vacuolar marker proteins. Immunogold EM confirms that prAPI is localized in cytosolic and in subvacuolar vesicles in a mutant strain defective in autophagic body degradation. These data suggest that cytosolic vesicles containing prAPI fuse with the vacuole to release a membrane-bounded intermediate compartment that is subsequently broken down, allowing API maturation.

Bibliography

Scott, S. V., Baba, M., Ohsumi, Y., & Klionsky, D. J. (1997). Aminopeptidase I Is Targeted to the Vacuole by a Nonclassical Vesicular Mechanism. The Journal of Cell Biology, 138(1), 37â44.

Authors 4

Sidney V. Scott (first)
Misuzu Baba (additional)
Yoshinori Ohsumi (additional)
Daniel J. Klionsky (additional)

References 30 Referenced 138

10.1083/jcb.124.6.903 / J Cell Biol / Ultrastructural analysis of the autophagic process in yeast: detection of autophagosomes and their characterization by Baba (1994)
10.1247/csf.20.465 / Cell Struct Funct / Analysis of the membrane structures involved in autophagy in yeast by freeze-replica method by Baba (1995)
10.1016/S0091-679X(08)61605-2 / Methods Cell Biol / Reconstitution of protein transport using broken yeast spheroplasts by Baker (1989)
10.1016/0092-8674(94)90138-4 / Cell / COPII: a membrane coat formed by Sec proteins that drive vesicle budding from the endoplasmic reticulum by Barlowe (1994)
10.1016/0092-8674(95)90088-8 / Cell / Vacuolar biogenesis in yeast: sorting out the sorting proteins by Conibear (1995)
10.1016/0962-8924(94)90069-8 / Trends Cell Biol / Autophagy and related mechanisms of lysosome-mediated protein degradation by Dunn (1995)
10.1083/jcb.131.3.591 / J Cell Biol / Isolation and characterization of yeast mutants in the cytoplasm to vacuole protein targeting pathway by Harding (1995)
10.1074/jbc.271.30.17621 / J Biol Chem / Genetic and phenotypic overlap between autophagy and the cytoplasm to vacuole protein targeting pathway by Harding (1996)
10.1111/j.1365-2958.1992.tb01419.x / Mol Microbiol / Stress-induced proteolysis in yeast by Hilt (1992)
10.1083/jcb.137.3.609 / J Cell Biol / Transport of a large oligomeric protein by the cytoplasm to vacuole protein targeting pathway by Kim (1997)
10.1002/j.1460-2075.1989.tb08348.x / EMBO (Eur Mol Biol Organ) J / Membrane protein sorting: biogenesis, transport and processing of yeast vacuolar alkaline phosphatase by Klionsky (1989)
{'key': '2023072822493889600_B12', 'first-page': '2105', 'article-title': 'Intracellular sorting and processing of a yeast vacuolar hydrolase: proteinase A propeptide contains vacuolar targeting information', 'volume': '8', 'author': 'Klionsky', 'year': '1988', 'journal-title': 'Mol Cell Biol'} / Mol Cell Biol / Intracellular sorting and processing of a yeast vacuolar hydrolase: proteinase A propeptide contains vacuolar targeting information by Klionsky (1988)
10.1128/mr.54.3.266-292.1990 / Microbiol Rev / The fungal vacuole: composition, function, and biogenesis by Klionsky (1990)
10.1083/jcb.119.2.287 / J Cell Biol / Aminopeptidase I of Saccharomyces cerevisiaeis localized to the vacuole independent of the secretory pathway by Klionsky (1992)
10.1006/bbrc.1995.1636 / Biochem Biophys Res Commun / Novel system for monitoring autophagy in the yeast Saccharomyces cerevisiae. by Noda (1995)
10.1016/0092-8674(80)90128-2 / Cell / Identification of 23 complementation groups required for post-translational events in the yeast secretory pathway by Novick (1980)
10.1083/jcb.132.6.999 / J Cell Biol / Identification of a cytoplasm to vacuole targeting determinant in aminopeptidase I by Oda (1996)
{'key': '2023072822493889600_B18', 'first-page': '5801', 'article-title': 'Isolation and characterization of PEP3, a gene required for vacuolar biogenesis in Saccharomyces cerevisiae.', 'volume': '11', 'author': 'Preston', 'year': '1991', 'journal-title': 'Mol Cell Biol'} / Mol Cell Biol / Isolation and characterization of PEP3, a gene required for vacuolar biogenesis in Saccharomyces cerevisiae. by Preston (1991)
10.1083/jcb.113.3.527 / J Cell Biol / Immunolocalization of Kex2 protease identifies a putative late Golgi compartment in the yeast Saccharomyces cerevisiae. by Redding (1991)
10.1016/0962-8924(93)90056-7 / Trends Cell Biol / Yeast endocytosis by Riezman (1993)
{'key': '2023072822493889600_B21', 'first-page': '4936', 'article-title': 'Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases', 'volume': '8', 'author': 'Robinson', 'year': '1988', 'journal-title': 'Mol Cell Biol'} / Mol Cell Biol / Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases by Robinson (1988)
{'key': '2023072822493889600_B22', 'first-page': '5813', 'article-title': 'A putative zinc finger protein, Saccharomyces cerevisiaeVps18p, affects late Golgi functions required for vacuolar protein sorting and efficient α-factor prohormone maturation', 'volume': '11', 'author': 'Robinson', 'year': '1991', 'journal-title': 'Mol Cell Biol'} / Mol Cell Biol / A putative zinc finger protein, Saccharomyces cerevisiaeVps18p, affects late Golgi functions required for vacuolar protein sorting and efficient α-factor prohormone maturation by Robinson (1991)
10.1083/jcb.131.6.1727 / J Cell Biol / In vitro reconstitution of cytoplasm to vacuole protein transport in yeast by Scott (1995)
10.1073/pnas.93.22.12304 / Proc Natl Acad Sci USA / Cytoplasm to vacuole targeting and autophagy employ the same machinery to deliver proteins to the yeast vacuole by Scott (1996)
10.1002/j.1460-2075.1995.tb00234.x / EMBO (Eur Mol Biol Organ) J / Yeast aminopeptidase I is post-translationally sorted from the cytosol to the vacuole by a mechanism mediated by its bipartite N-terminal extension by Segui-Real (1995)
10.1146/annurev.cb.11.110195.000245 / Annu Rev Cell Dev Biol / Receptor-mediated protein sorting to the vacuole in yeast: roles for a protein kinase, a lipid kinase and GTP-binding proteins by Stack (1995)
10.1091/mbc.3.2.129 / Mol Biol Cell / Protein translocation mutants defective in the insertion of integral membrane proteins into the endoplasmic reticulum by Stirling (1992)
10.1083/jcb.119.2.301 / J Cell Biol / Autophagy in yeast demonstrated with proteinase-deficient mutants and conditions for its induction by Takeshige (1992)
10.1016/0014-5793(94)00672-5 / FEBS Lett / Isolation of autophagocytosis mutants of Saccharomyces cerevisiae. by Thumm (1994)
10.1016/0014-5793(93)80398-E / FEBS Lett / Isolation and characterization of autophagy-defective mutants of Saccharomyces cerevisiae. by Tsukada (1993)

Dates

Type	When
Created	23 years ago (July 26, 2002, 12:47 p.m.)
Deposited	2 years ago (July 28, 2023, 6:50 p.m.)
Indexed	2 weeks, 6 days ago (Aug. 5, 2025, 9:03 a.m.)
Issued	28 years, 1 month ago (July 14, 1997)
Published	28 years, 1 month ago (July 14, 1997)
Published Online	28 years, 1 month ago (July 14, 1997)
Published Print	28 years, 1 month ago (July 14, 1997)

Funders 0

None

BibTeX

@article{Scott_1997, title={Aminopeptidase I Is Targeted to the Vacuole by a Nonclassical Vesicular Mechanism}, volume={138}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.138.1.37}, DOI={10.1083/jcb.138.1.37}, number={1}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Scott, Sidney V. and Baba, Misuzu and Ohsumi, Yoshinori and Klionsky, Daniel J.}, year={1997}, month=jul, pages={37–44} }

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