DOI: 10.1083/jcb.138.1.119. Control of Mitotic Events by Nap1 and the Gin4 Kinase

Control of Mitotic Events by Nap1 and the Gin4 Kinase

10.1083/jcb.138.1.119

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Little is known about the pathways used by cyclins and cyclin-dependent kinases to induce the events of the cell cycle. In budding yeast, a protein called Nap1 binds to the mitotic cyclin Clb2, and Nap1 is required for the ability of Clb2 to induce specific mitotic events, but the role played by Nap1 is unclear. We have used genetic and biochemical approaches to identify additional proteins that function with Nap1 in the control of mitotic events. These approaches have both identified a protein kinase called Gin4 that is required for the ability of Clb2 and Nap1 to promote the switch from polar to isotropic bud growth that normally occurs during mitosis. Gin4 is also required for the ability of Clb2 and Nap1 to promote normal progression through mitosis. The Gin4 protein becomes phosphorylated as cells enter mitosis, resulting in the activation of Gin4 kinase activity, and the phosphorylation of Gin4 is dependent upon Nap1 and Clb2 in vivo. Affinity chromatography experiments demonstrate that Gin4 binds tightly to Nap1, indicating that the functions of these two proteins are closely tied within the cell. These results demonstrate that the activation of Gin4 is under the control of Clb2 and Nap1, and they provide an important step towards elucidating the molecular pathways that link cyclin-dependent kinases to the events they control.

Bibliography

Altman, R., & Kellogg, D. (1997). Control of Mitotic Events by Nap1 and the Gin4 Kinase. The Journal of Cell Biology, 138(1), 119â130.

Authors 2

Roger Altman (first)
Douglas Kellogg (additional)

References 33 Referenced 137

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10.1016/0092-8674(94)90443-X / Cell / Closing the cell cycle circle in yeast: G2 cyclin proteolysis initiated at mitosis persists until the activation of G1 cyclins in the next cycle by Amon (1994)
10.1128/jvi.12.2.241-252.1973 / J Virol / Processing of adenovirus 2-induced proteins by Anderson (1973)
10.1091/mbc.3.7.805 / Mol Biol Cell / Characterization of four B-type cyclin genes of the budding yeast Saccharomyces cerevisiae. by Fitch (1992)
10.1016/0092-8674(91)90417-W / Cell / A cyclin B homolog in S. cerevisiae: chronic activation of the Cdc28 protein kinase by cyclin prevents exit from mitosis by Ghiara (1991)
10.1002/j.1460-2075.1991.tb07809.x / EMBO (Eur Mol Biol Organ) J / XenopusM phase MAP kinase: isolation of its cDNA and activation by MPF by Gotoh (1991)
10.1016/0076-6879(91)00126-H / Methods Enzymol / Protein kinase catalytic domain sequence database: identification of conserved features of primary structure and classification of family members by Hanks (1991)
{'key': '2023072822473802400_B8'}
10.1016/S0092-8674(00)81080-6 / Cell / The yeast CDC16 and CDC27 genes restrict DNA replication to once per cell cycle by Heichman (1996)
10.1111/j.1432-1033.1994.tb19966.x / Eur J Biochem / A 40-kDa myelin basic protein kinase, distinct from erk1 and erk2, is activated in mitotic HeLa cells by Heider (1994)
10.1016/0092-8674(93)90364-V / Cell / Anaphase is initiated by proteolysis rather than by the inactivation of MPF by Holloway (1993)
10.1016/0092-8674(81)90014-3 / Cell / S. cerevisiaegenes required for cell cycle arrest in response to loss of microtubule function by Hoyt (1991)
10.1016/0092-8674(95)90337-2 / Cell / Genes involved in sister chromatid separation are needed for B-type cyclin proteolysis in budding yeast by Irniger (1995)
10.1091/mbc.3.1.1 / Mol Biol Cell / Purification of a multiprotein complex containing centrosomal proteins from the Drosophila embryo by chromatography with low-affinity polyclonal antibodies by Kellogg (1992)
10.1083/jcb.130.3.675 / J Cell Biol / NAP1 acts with Clb2 to perform mitotic functions and suppress polar bud growth in budding yeast by Kellogg (1995)
10.1083/jcb.130.3.661 / J Cell Biol / Members of the NAP/SET family of proteins interact specifically with B-type cyclins by Kellogg (1995)
10.1091/mbc.6.12.1673 / Mol Biol Cell / CP60: a microtubule-associated protein that is localized to the centrosome in a cell cycle-specific manner by Kellogg (1995)
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10.1016/0092-8674(95)90338-0 / Cell / A 20S complex containing CDC27 and CDC16 catalyzes the mitosis specific conjugation of ubiquitin to cyclin B by King (1995)
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10.1083/jcb.120.6.1305 / J Cell Biol / Morphogenesis in the yeast cell cycle: regulation by Cdc28 and cyclins by Lew (1993)
10.1016/S0959-437X(95)90048-9 / Curr Opin Gen Dev / Cell cycle control of morphogenesis in budding yeast by Lew (1995)
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{'key': '2023072822473802400_B27'}
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10.1016/0076-6879(91)94043-C / Methods Enzymol / Immunofluorescence methods for yeast by Pringle (1991)
10.1101/gad.6.11.2021 / Genes & Dev / Cyclin B homologs in Saccharomyces cerevisiaefunction in S phase and in G2 by Richardson (1992)
10.1083/jcb.127.5.1301 / J Cell Biol / Anaphase onset in vertebrate somatic cells is controlled by a checkpoint that monitors sister kinetochore attachment to the spindle by Rieder (1994)

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:47 p.m.)
Deposited	2 years, 1 month ago (July 28, 2023, 6:48 p.m.)
Indexed	2 months ago (July 2, 2025, 3:07 p.m.)
Issued	28 years, 1 month ago (July 14, 1997)
Published	28 years, 1 month ago (July 14, 1997)
Published Online	28 years, 1 month ago (July 14, 1997)
Published Print	28 years, 1 month ago (July 14, 1997)

Funders 0

None

BibTeX

@article{Altman_1997, title={Control of Mitotic Events by Nap1 and the Gin4 Kinase}, volume={138}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.138.1.119}, DOI={10.1083/jcb.138.1.119}, number={1}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Altman, Roger and Kellogg, Douglas}, year={1997}, month=jul, pages={119–130} }

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  "journal-issue": {
    "issue": "1",
    "published-print": {
      "date-parts": [
        [
          1997,
          7,
          14
        ]
      ]
    }
  },
  "URL": "http://dx.doi.org/10.1083/jcb.138.1.119",
  "relation": {},
  "ISSN": [
    "0021-9525",
    "1540-8140"
  ],
  "subject": [],
  "published": {
    "date-parts": [
      [
        1997,
        7,
        14
      ]
    ]
  }
}