DOI: 10.1083/jcb.137.7.1511. The Yeast v-SNARE Vti1p Mediates Two Vesicle Transport Pathways through Interactions with the t-SNAREs Sed5p and Pep12p

The Yeast v-SNARE Vti1p Mediates Two Vesicle Transport Pathways through Interactions with the t-SNAREs Sed5p and Pep12p

10.1083/jcb.137.7.1511

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Membrane traffic in eukaryotic cells requires that specific v-SNAREs on transport vesicles interact with specific t-SNAREs on target membranes. We identified a novel Saccharomyces cerevisiae v-SNARE (Vti1p) encoded by the essential gene, VTI1. Vti1p interacts with the prevacuolar t-SNARE Pep12p to direct Golgi to prevacuolar traffic. vti1-1 mutant cells missorted and secreted the soluble vacuolar hydrolase carboxypeptidase Y (CPY) rapidly and reversibly when vti1-1 cells were shifted to the restrictive temperature. However, overexpression of Pep12p suppressed the CPY secretion defect exhibited by vti1-1 cells at 36°C. Characterization of a second vti1 mutant, vti1-11, revealed that Vti1p also plays a role in membrane traffic at a cis-Golgi stage. vti1-11 mutant cells displayed a growth defect and accumulated the ER and early Golgi forms of both CPY and the secreted protein invertase at the nonpermissive temperature. Overexpression of the yeast cis-Golgi t-SNARE Sed5p suppressed the accumulation of the ER form of CPY but did not lead to CPY transport to the vacuole in vti1-11 cells. Overexpression of Sed5p allowed growth in the absence of Vti1p. In vitro binding and coimmunoprecipitation studies revealed that Vti1p interacts directly with the two t-SNAREs, Sed5p and Pep12p. These data suggest that Vti1p plays a role in cis-Golgi membrane traffic, which is essential for yeast viability, and a nonessential role in the fusion of Golgi-derived vesicles with the prevacuolar compartment. Therefore, a single v-SNARE can interact functionally with two different t-SNAREs in directing membrane traffic in yeast.

Bibliography

Mollard, G. F. von, Nothwehr, S. F., & Stevens, T. H. (1997). The Yeast v-SNARE Vti1p Mediates Two Vesicle Transport Pathways through Interactions with the t-SNAREs Sed5p and Pep12p. The Journal of Cell Biology, 137(7), 1511â1524.

Authors 3

Gabriele Fischer von Mollard (first)
Steven F. Nothwehr (additional)
Tom H. Stevens (additional)

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Dates

Type	When
Created	23 years ago (July 26, 2002, 12:47 p.m.)
Deposited	2 years ago (July 28, 2023, 7:53 p.m.)
Indexed	4 days, 5 hours ago (Aug. 19, 2025, 6:15 a.m.)
Issued	28 years, 1 month ago (June 30, 1997)
Published	28 years, 1 month ago (June 30, 1997)
Published Online	28 years, 1 month ago (June 30, 1997)
Published Print	28 years, 1 month ago (June 30, 1997)

Funders 0

None

BibTeX

@article{Mollard_1997, title={The Yeast v-SNARE Vti1p Mediates Two Vesicle Transport Pathways through Interactions with the t-SNAREs Sed5p and Pep12p}, volume={137}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.137.7.1511}, DOI={10.1083/jcb.137.7.1511}, number={7}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Mollard, Gabriele Fischer von and Nothwehr, Steven F. and Stevens, Tom H.}, year={1997}, month=jun, pages={1511–1524} }

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