DOI: 10.1083/jcb.137.1.247. Substrate Recognition by Osteoclast Precursors Induces C-src/Microtubule Association

Substrate Recognition by Osteoclast Precursors Induces C-src/Microtubule Association

10.1083/jcb.137.1.247

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

The osteoclast is distinguished from other macrophage polykaryons by its polarization, a feature induced by substrate recognition. The most striking component of the polarized osteoclast is its ruffled membrane, probably reflecting insertion of intracellular vesicles into the bone apposed plasmalemma. The failure of osteoclasts in c-src−/− osteopetrotic mice to form ruffled membranes indicates pp60c-src (c-src) is essential to osteoclast polarization. Interestingly, c-src itself is a vesicular protein that targets the ruffled membrane. This being the case, we hypothesized that matrix recognition by osteoclasts, and their precursors, induces c-src to associate with microtubules that traffic proteins to the cell surface. We find abundant c-src associates with tubulin immunoprecipitated from avian marrow macrophages (osteoclast precursors) maintained in the adherent, but not nonadherent, state. Since the two proteins colocalize only within adherent avian osteoclast-like cells examined by double antibody immunoconfocal microscopy, c-src/tubulin association reflects an authentic intracellular event. C-src/tubulin association is evident within 90 min of cell-substrate recognition, and the event does not reflect increased expression of either protein. In vitro kinase assay demonstrates tubulin-associated c-src is enzymatically active, phosphorylating itself as well as exogenous substrate. The increase in microtubule-associated kinase activity attending adhesion mirrors tubulin-bound c-src and does not reflect enhanced specific activity. The fact that microtubule-dissociating drugs, as well as cold, prevent adherence-induced c-src/tubulin association indicates the protooncogene complexes primarily, if not exclusively, with polymerized tubulin. Association of the two proteins does not depend upon protein tyrosine phosphorylation and is substrate specific, as it is induced by vitronectin and fibronectin but not type 1 collagen. Finally, consistent with cotransport of c-src and the osteoclast vacuolar proton pump to the polarized plasmalemma, the H+-ATPase decorates microtubules in a manner similar to the protooncogene, specifically coimmunoprecipitates with c-src from the osteoclast light Golgi membrane fraction, and is present, with c-src, in preparations enriched with acidifying vesicles reconstituted from the osteoclast ruffled membrane.

Bibliography

Abu-Amer, Y., Ross, F. P., Schlesinger, P., Tondravi, M. M., & Teitelbaum, S. L. (1997). Substrate Recognition by Osteoclast Precursors Induces C-src/Microtubule Association. The Journal of Cell Biology, 137(1), 247â258.

Authors 5

Yousef Abu-Amer (first)
F. Patrick Ross (additional)
Paul Schlesinger (additional)
M. Mehrdad Tondravi (additional)
Steven L. Teitelbaum (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:47 p.m.)
Deposited	2 years, 1 month ago (July 28, 2023, 6:58 p.m.)
Indexed	1 year, 3 months ago (May 12, 2024, 6:36 p.m.)
Issued	28 years, 4 months ago (April 7, 1997)
Published	28 years, 4 months ago (April 7, 1997)
Published Online	28 years, 4 months ago (April 7, 1997)
Published Print	28 years, 4 months ago (April 7, 1997)

Funders 0

None

BibTeX

@article{Abu_Amer_1997, title={Substrate Recognition by Osteoclast Precursors Induces C-src/Microtubule Association}, volume={137}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.137.1.247}, DOI={10.1083/jcb.137.1.247}, number={1}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Abu-Amer, Yousef and Ross, F. Patrick and Schlesinger, Paul and Tondravi, M. Mehrdad and Teitelbaum, Steven L.}, year={1997}, month=apr, pages={247–258} }

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