DOI: 10.1083/jcb.136.6.1185. In Vivo Dynamics of Nuclear Pore Complexes in Yeast

In Vivo Dynamics of Nuclear Pore Complexes in Yeast

10.1083/jcb.136.6.1185

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

While much is known about the role of nuclear pore complexes (NPCs) in nucleocytoplasmic transport, the mechanism of NPC assembly into pores formed through the double lipid bilayer of the nuclear envelope is not well defined. To investigate the dynamics of NPCs, we developed a live-cell assay in the yeast Saccharomyces cerevisiae. The nucleoporin Nup49p was fused to the green fluorescent protein (GFP) of Aequorea victoria and expressed in nup49 null haploid yeast cells. When the GFP–Nup49p donor cell was mated with a recipient cell harboring only unlabeled Nup49p, the nuclei fused as a consequence of the normal mating process. By monitoring the distribution of the GFP–Nup49p, we could assess whether NPCs were able to move from the donor section of the nuclear envelope to that of the recipient nucleus. We observed that fluorescent NPCs moved and encircled the entire nucleus within 25 min after fusion. When assays were done in mutant kar1-1 strains, where nuclear fusion does not occur, GFP–Nup49p appearance in the recipient nucleus occurred at a very slow rate, presumably due to new NPC biogenesis or to exchange of GFP– Nup49p into existing recipient NPCs. Interestingly, in a number of existing mutant strains, NPCs are clustered together at permissive growth temperatures. This has been explained with two different hypotheses: by movement of NPCs through the double nuclear membranes with subsequent clustering at a central location; or, alternatively, by assembly of all NPCs at a central location (such as the spindle pole body) with NPCs in mutant cells unable to move away from this point. Using the GFP–Nup49p system with a mutant in the NPCassociated factor Gle2p that exhibits formation of NPC clusters only at 37°C, it was possible to distinguish between these two models for NPC dynamics. GFP– Nup49p-labeled NPCs, assembled at 23°C, moved into clusters when the cells were shifted to growth at 37°C. These results indicate that NPCs can move through the double nuclear membranes and, moreover, can do so to form NPC clusters in mutant strains. Such clusters may result by releasing NPCs from a nuclear tether, or by disappearance of a protein that normally prevents pore aggregation. This system represents a novel approach for identifying regulators of NPC assembly and movement in the future.

Bibliography

Bucci, M., & Wente, S. R. (1997). In Vivo Dynamics of Nuclear Pore Complexes in Yeast. The Journal of Cell Biology, 136(6), 1185â1199.

Authors 2

Mirella Bucci (first)
Susan R. Wente (additional)

References 76 Referenced 99

10.1083/jcb.131.6.1659 / J Cell Biol / Nup120p: a yeast nucleoporin required for NPC distribution and mRNA transport by Aitchison (1995)
10.1083/jcb.131.5.1133 / J Cell Biol / Two novel related yeast nucleoporins Nup170p and Nup157p : complementation with the vertebrate homologue Nup155p and functional interactions with the yeast nuclear pore–membrane protein Pom152p by Aitchison (1995)
10.1016/S0022-2836(95)80050-6 / J Mol Biol / Structural plasticity of the nuclear pore complex by Akey (1995)
10.1083/jcb.108.6.2059 / J Cell Biol / Yeast nuclear envelope proteins cross-react with an antibody against mammalian pore complex proteins by Aris (1989)
10.1093/nar/21.14.3329 / Nucleic Acids Res / A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae. by Baudin (1993)
10.1083/jcb.116.2.281 / J Cell Biol / GTP hydrolysis is required for vesicle fusion during nuclear envelope assembly in vitro by Boman (1992)
10.1038/358512a0 / Nature (Lond) / A role for ADP-ribosylation factor in nuclear vesicle dynamics by Boman (1992)
10.1016/0378-1119(92)90454-W / Gene (Amst) / Multifunctional yeast high-copy-number shuttle vectors by Christianson (1992)
10.1073/pnas.73.10.3651 / Proc Natl Acad Sci USA / A mutant of Saccharomyces cerevisiaedefective for nuclear fusion by Conde (1976)
10.1083/jcb.112.6.1073 / J Cell Biol / Spontaneous assembly of pore complex-containing membranes (“annulate lamellae”) in Xenopusegg extract in the absence of chromatin by Dabauvalle (1991)
10.1007/BF00337603 / Chromosoma (Berl) / Identification of a soluble precursor complex essential for nuclear pore assembly in vitro by Dabauvalle (1990)
10.1146/annurev.bi.64.070195.004245 / Annu Rev Biochem / The nuclear pore complex by Davis (1995)
10.1002/j.1460-2075.1994.tb06953.x / EMBO (Eur Mol Biol Organ) J / A novel nuclear pore protein Nup133p with distinct roles in poly(A)+RNA transport and nuclear pore distribution by Doye (1994)
{'key': '2023072206424392900_B14'}
10.1016/0092-8674(90)90712-N / Cell / Reconstitution of biochemically altered nuclear pores: transport can be eliminated and restored by Finlay (1990)
10.1083/jcb.114.1.169 / J Cell Biol / A complex of nuclear pore proteins required for pore function by Finlay (1991)
10.1146/annurev.cb.08.110192.002431 / Annu Rev Cell Biol / Structure and function of the nuclear pore complex by Forbes (1992)
10.1016/0962-8924(94)90067-1 / Trends Cell Biol / Integral membrane proteins and dynamic organization of the nuclear envelope by Gerace (1994)
10.1016/0955-0674(95)80083-2 / Curr Opin Cell Biol / Structural and functional organization of the nuclear envelope by Goldberg (1995)
10.1091/mbc.7.6.917 / Mol Biol Cell / Pleiotropic nuclear defects associated with a conditional allele of the novel nucleoporin Rat9p/Nup85p by Goldstein (1996)
10.1126/science.271.5255.1513 / Science (Wash DC) / Nucleocytoplasmic transport by Gorlich (1996)
10.1083/jcb.129.4.939 / J Cell Biol / A conditional allele of the novel repeat-containing yeast nucleoporin RAT7/NUP159causes both rapid cessation of mRNA export and reversible clustering of nuclear pore complexes by Gorsch (1995)
10.1002/j.1460-2075.1993.tb05975.x / EMBO (Eur Mol Biol Organ) J / Purification of NSP1 reveals complex formation with ‘GLFG' nucleoporins and a novel nuclear pore protein NIC96 by Grandi (1993)
10.1073/pnas.93.2.828 / Proc Natl Acad Sci USA / In vivo examination of membrane protein localization and degradation with green fluorescent protein by Hampton (1996)
10.1083/jcb.131.6.1677 / J Cell Biol / Nuclear pore complex clustering and nuclear accumulation of poly(A)+ RNA associated with mutation of the Saccharomyces cerevisiae rat2/nup120gene by Heath (1995)
10.1016/S0960-9822(02)00450-5 / Curr Biol / Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer by Heim (1996)
10.1083/jcb.131.6.1699 / J Cell Biol / The GLFG repetitive region of the nucleoporin Nup116p interacts with Kap95p, an essential yeast nuclear import factor by Iovine (1995)
10.1128/jb.153.1.163-168.1983 / J Bacteriol / Transformation of intact yeast cells treated with alkali cations by Ito (1983)
10.1016/0014-4827(77)90114-8 / Exp Cell Res / Nuclear pore formation and the cell cycle in Saccharomyces cerevisiae. by Jordan (1977)
10.1073/pnas.92.21.9707 / Proc Natl Acad Sci USA / Kinetics of spindle pole body separation in budding yeast by Kahana (1995)
10.1016/S0074-7696(08)60826-8 / Int Rev Cytol / The structure and function of annulate lamellae: porous cytoplasmic and intranuclear membranes by Kessel (1983)
10.1083/jcb.126.4.911 / J Cell Biol / Nuclear congression and membrane fusion: two distinct events in the yeast karyogamy pathway by Kurihara (1994)
10.1016/0092-8674(94)90575-4 / Cell / The karyogamy gene KAR2and novel proteins are required for ER-membrane fusion by Latterich (1994)
10.1091/mbc.6.4.401 / Mol Biol Cell / Mutation or deletion of the Saccharomyces cerevisiae RAT3/NUP133 gene causes temperature-dependent nuclear accumulation of poly(A)+RNA and constitutive clustering of nuclear pore complexes by Li (1995)
10.1126/science.6601299 / Science (Wash DC) / Formation in vitro of sperm pronuclei and mitotic chromosomes induced by amphibian ooplasmic components by Lohka (1983)
10.1083/jcb.132.1.5 / J Cell Biol / Assembly of the nuclear pore: biochemically distinct steps revealed with NEM, GTPγS, and BAPTA by Macaulay (1996)
{'key': '2023072206424392900_B37', 'first-page': '75', 'article-title': 'The nuclear and the cytoplasmic pore complex: structure, dynamics, distribution, and evolution', 'volume': '5', 'author': 'Maul', 'year': '1977', 'journal-title': 'Int Rev Cytol (Suppl)'} / Int Rev Cytol (Suppl) / The nuclear and the cytoplasmic pore complex: structure, dynamics, distribution, and evolution by Maul (1977)
10.1083/jcb.129.6.1459 / J Cell Biol / Nuclear pore complex assembly studied with a biochemical assay for annulate lamellae formation by Meier (1995)
10.1016/0955-0674(95)80084-0 / Curr Opin Cell Biol / Mechanisms of nuclear protein import by Melchior (1995)
10.1016/0092-8674(90)90351-E / Cell / KAR3, a kinesin-related gene required for yeast nuclear fusion by Meluh (1990)
10.1091/mbc.7.12.1921 / Mol Biol Cell / GLE2, a S. cerevisiae homologue of the S. pombe export factor RAE1, is required for nuclear pore complex structure and function by Murphy (1996)
{'key': '2023072206424392900_B42', 'first-page': '280', 'article-title': 'NSP1 depletion in yeast affects nuclear pore formation and nuclear accumulation', 'volume': '59', 'author': 'Mutvei', 'year': '1992', 'journal-title': 'Eur J Cell Biol'} / Eur J Cell Biol / NSP1 depletion in yeast affects nuclear pore formation and nuclear accumulation by Mutvei (1992)
10.1016/0092-8674(90)90063-K / Cell / NSP1: a yeast nuclear envelope protein localized at the nuclear pores exerts its essential function by its carboxy-terminal domain by Nehrbass (1990)
10.1083/jcb.110.3.547 / J Cell Biol / A N-ethylmaleimide-sensitive cytosolic factor necessary for nuclear protein import: requirement in signal- mediated binding to the nuclear pore by Newmeyer (1990)
10.1016/0092-8674(87)90424-7 / Cell / Nuclear reconstitution in vitro: stages of assembly around protein-free DNA by Newport (1987)
10.1083/jcb.116.2.295 / J Cell Biol / Characterization of the membrane binding and fusion events during nuclear envelope assembly using purified components by Newport (1992)
10.1146/annurev.bi.62.070193.001251 / Annu Rev Biochem / Nucleocytoplasmic transport in the yeast Saccharomyces cerevisiae. by Osborne (1993)
10.3109/10409239609106583 / Crit Rev Biochem Mol Biol / Molecular dissection of the nuclear pore complex by Pante (1996)
10.1073/pnas.92.4.1187 / Proc Natl Acad Sci USA / Disruption of the nucleoporin gene NUP133results in clustering of nuclear pore complexes by Pemberton (1995)
10.1016/0092-8674(91)90155-R / Cell / Assembly/disassembly of the nuclear envelope membrane: cell cycle-dependent binding of nuclear membrane vesicles to chromatin in vitro by Pfaller (1991)
{'key': '2023072206424392900_B51', 'first-page': '54', 'article-title': 'The three dimensional structure of the nuclear pore complex as seen by high voltage electron microscopy and high resolution low voltage scanning electron microscopy', 'volume': '21', 'author': 'Ris', 'year': '1991', 'journal-title': 'EMSA Bull'} / EMSA Bull / The three dimensional structure of the nuclear pore complex as seen by high voltage electron microscopy and high resolution low voltage scanning electron microscopy by Ris (1991)
10.1016/0092-8674(87)90712-4 / Cell / KAR1, a gene required for function of both intranuclear and extranuclear microtubules in yeast by Rose (1987)
10.1083/jcb.123.4.771 / J Cell Biol / Isolation of the yeast nuclear pore complex by Rout (1993)
{'key': '2023072206424392900_B54'}
10.1016/S0022-5320(76)80023-8 / J Ultrastruct Res / Nuclear pore absence from areas of close association between nucleus and vacuole in synchronous yeast cultures by Severs (1976)
10.1083/jcb.106.1.1 / J Cell Biol / Steps in the assembly of replication-competent nuclei in a cell-free system from Xenopuseggs by Sheehan (1988)
{'key': '2023072206424392900_B57'}
10.1093/genetics/122.1.19 / Genetics / A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. by Sikorski (1989)
10.1016/S0092-8674(00)80981-2 / Cell / A novel complex of nucleoporins, which includes Sec13p and a Sec13p homolog, is essential for normal nuclear pores by Siniossoglou (1996)
10.1073/pnas.91.21.10124 / Proc Natl Acad Sci USA / Colocalization of vertebrate lamin B and lamin B receptor (LBR) in nuclear envelopes and in LBR-induced membrane stacks of the yeast Saccharomyces cerevisiae. by Smith (1994)
10.1016/S0960-9822(95)00056-X / Curr Biol / Green fluorescent protein. The green revolution by Stearns (1995)
10.1016/1043-4682(92)90028-T / Semin Cell Biol / Nuclear pore structure and function by Stewart (1992)
10.1016/0092-8674(93)90366-X / Cell / Calcium mobilization is required for nuclear vesicle fusion in vitro: implications for membrane traffic and IP3 receptor function by Sullivan (1993)
10.1002/j.1460-2075.1990.tb08084.x / EMBO (Eur Mol Biol Organ) J / Characterization of nuclear localizing sequences derived from yeast ribosomal protein L29 by Underwood (1990)
10.1083/jcb.117.6.1277 / J Cell Biol / Separate domains of KAR1 mediate distinct functions in mitosis and nuclear fusion by Vallen (1992)
10.1083/jcb.112.4.545 / J Cell Biol / A distinct vesicle population targets membranes and pore complexes to the nuclear envelope in Xenopuseggs by Vigers (1991)
10.1242/jcs.102.2.273 / J Cell Sci / Regulation of nuclear envelope precursor functions during cell division by Vigers (1992)
10.1083/jcb.132.5.861 / J Cell Biol / Movement of cortical actin patches in yeast by Waddle (1996)
10.1083/jcb.123.2.275 / J Cell Biol / A temperature–sensitive NUP116null mutant forms a nuclear envelope seal over the yeast nuclear pore complex thereby blocking nucleocytoplasmic traffic by Wente (1993)
10.1083/jcb.125.5.955 / J Cell Biol / NUP145encodes a novel yeast glycine-leucine-phenylalanine-glycine (GLFG) nucleoporin required for nuclear envelope structure by Wente (1994)
{'key': '2023072206424392900_B71'}
10.1083/jcb.119.4.705 / J Cell Biol / A new family of yeast nuclear pore complex proteins by Wente (1992)
10.1016/0955-0674(93)90002-8 / Curr Opin Cell Biol / Nuclear membrane dynamics by Wiese (1993)
10.1083/jcb.107.1.101 / J Cell Biol / Increased amounts of HMG–CoA reductase induce “karmellae”: a proliferation of stacked membrane pairs surrounding the yeast nucleus by Wright (1988)
10.1083/jcb.130.3.687 / J Cell Biol / Spindle dynamics and cell cycle regulation of dynein in the budding yeast, Saccharomyces cerevisiae. by Yeh (1995)
10.1083/jcb.133.6.1141 / J Cell Biol / Nic96p is required for nuclear pore formation and functionally interacts with a novel nucleoporin, Nup188p by Zabel (1996)

Dates

Type	When
Created	23 years ago (July 26, 2002, 12:47 p.m.)
Deposited	2 years ago (July 22, 2023, 2:44 a.m.)
Indexed	1 week, 6 days ago (Aug. 7, 2025, 4:22 p.m.)
Issued	28 years, 4 months ago (March 24, 1997)
Published	28 years, 4 months ago (March 24, 1997)
Published Online	28 years, 4 months ago (March 24, 1997)
Published Print	28 years, 4 months ago (March 24, 1997)

Funders 0

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BibTeX

@article{Bucci_1997, title={In Vivo Dynamics of Nuclear Pore Complexes in Yeast}, volume={136}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.136.6.1185}, DOI={10.1083/jcb.136.6.1185}, number={6}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Bucci, Mirella and Wente, Susan R.}, year={1997}, month=mar, pages={1185–1199} }

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  "abstract": "<jats:p>While much is known about the role of nuclear pore complexes (NPCs) in nucleocytoplasmic transport, the mechanism of NPC assembly into pores formed through the double lipid bilayer of the nuclear envelope is not well defined. To investigate the dynamics of NPCs, we developed a live-cell assay in the yeast Saccharomyces cerevisiae. The nucleoporin Nup49p was fused to the green fluorescent protein (GFP) of Aequorea victoria and expressed in nup49 null haploid yeast cells. When the GFP\u2013Nup49p donor cell was mated with a recipient cell harboring only unlabeled Nup49p, the nuclei fused as a consequence of the normal mating process. By monitoring the distribution of the GFP\u2013Nup49p, we could assess whether NPCs were able to move from the donor section of the nuclear envelope to that of the recipient nucleus. We observed that fluorescent NPCs moved and encircled the entire nucleus within 25 min after fusion. When assays were done in mutant kar1-1 strains, where nuclear fusion does not occur, GFP\u2013Nup49p appearance in the recipient nucleus occurred at a very slow rate, presumably due to new NPC biogenesis or to exchange of GFP\u2013 Nup49p into existing recipient NPCs. Interestingly, in a number of existing mutant strains, NPCs are clustered together at permissive growth temperatures. This has been explained with two different hypotheses: by movement of NPCs through the double nuclear membranes with subsequent clustering at a central location; or, alternatively, by assembly of all NPCs at a central location (such as the spindle pole body) with NPCs in mutant cells unable to move away from this point. Using the GFP\u2013Nup49p system with a mutant in the NPCassociated factor Gle2p that exhibits formation of NPC clusters only at 37\u00b0C, it was possible to distinguish between these two models for NPC dynamics. GFP\u2013 Nup49p-labeled NPCs, assembled at 23\u00b0C, moved into clusters when the cells were shifted to growth at 37\u00b0C. These results indicate that NPCs can move through the double nuclear membranes and, moreover, can do so to form NPC clusters in mutant strains. Such clusters may result by releasing NPCs from a nuclear tether, or by disappearance of a protein that normally prevents pore aggregation. This system represents a novel approach for identifying regulators of NPC assembly and movement in the future.</jats:p>",
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      "first-page": "1659",
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      "article-title": "Nup120p: a yeast nucleoporin required for NPC distribution and mRNA transport",
      "volume": "131",
      "author": "Aitchison",
      "year": "1995",
      "journal-title": "J Cell Biol"
    },
    {
      "key": "2023072206424392900_B2",
      "doi-asserted-by": "crossref",
      "first-page": "1133",
      "DOI": "10.1083/jcb.131.5.1133",
      "article-title": "Two novel related yeast nucleoporins Nup170p and Nup157p : complementation with the vertebrate homologue Nup155p and functional interactions with the yeast nuclear pore\u2013membrane protein Pom152p",
      "volume": "131",
      "author": "Aitchison",
      "year": "1995",
      "journal-title": "J Cell Biol"
    },
    {
      "key": "2023072206424392900_B3",
      "doi-asserted-by": "crossref",
      "first-page": "273",
      "DOI": "10.1016/S0022-2836(95)80050-6",
      "article-title": "Structural plasticity of the nuclear pore complex",
      "volume": "248",
      "author": "Akey",
      "year": "1995",
      "journal-title": "J Mol Biol"
    },
    {
      "key": "2023072206424392900_B4",
      "doi-asserted-by": "crossref",
      "first-page": "2059",
      "DOI": "10.1083/jcb.108.6.2059",
      "article-title": "Yeast nuclear envelope proteins cross-react with an antibody against mammalian pore complex proteins",
      "volume": "108",
      "author": "Aris",
      "year": "1989",
      "journal-title": "J Cell Biol"
    },
    {
      "key": "2023072206424392900_B5",
      "doi-asserted-by": "crossref",
      "first-page": "3329",
      "DOI": "10.1093/nar/21.14.3329",
      "article-title": "A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae.",
      "volume": "21",
      "author": "Baudin",
      "year": "1993",
      "journal-title": "Nucleic Acids Res"
    },
    {
      "key": "2023072206424392900_B6",
      "doi-asserted-by": "crossref",
      "first-page": "281",
      "DOI": "10.1083/jcb.116.2.281",
      "article-title": "GTP hydrolysis is required for vesicle fusion during nuclear envelope assembly in vitro",
      "volume": "116",
      "author": "Boman",
      "year": "1992",
      "journal-title": "J Cell Biol"
    },
    {
      "key": "2023072206424392900_B7",
      "doi-asserted-by": "crossref",
      "first-page": "512",
      "DOI": "10.1038/358512a0",
      "article-title": "A role for ADP-ribosylation factor in nuclear vesicle dynamics",
      "volume": "358",
      "author": "Boman",
      "year": "1992",
      "journal-title": "Nature (Lond)"
    },
    {
      "key": "2023072206424392900_B8",
      "doi-asserted-by": "crossref",
      "first-page": "119",
      "DOI": "10.1016/0378-1119(92)90454-W",
      "article-title": "Multifunctional yeast high-copy-number shuttle vectors",
      "volume": "110",
      "author": "Christianson",
      "year": "1992",
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    {
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      "article-title": "A mutant of Saccharomyces cerevisiaedefective for nuclear fusion",
      "volume": "73",
      "author": "Conde",
      "year": "1976",
      "journal-title": "Proc Natl Acad Sci USA"
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      "volume": "112",
      "author": "Dabauvalle",
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      "author": "Dabauvalle",
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      "journal-title": "Chromosoma (Berl)"
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      "first-page": "865",
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      "author": "Davis",
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      "journal-title": "Annu Rev Biochem"
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      "first-page": "6062",
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      "volume": "13",
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      "author": "Finlay",
      "year": "1990",
      "journal-title": "Cell"
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    {
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      "first-page": "169",
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      "article-title": "A complex of nuclear pore proteins required for pore function",
      "volume": "114",
      "author": "Finlay",
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      "journal-title": "J Cell Biol"
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      "article-title": "Structure and function of the nuclear pore complex",
      "volume": "8",
      "author": "Forbes",
      "year": "1992",
      "journal-title": "Annu Rev Cell Biol"
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      "author": "Gerace",
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      "volume": "7",
      "author": "Goldberg",
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      "volume": "7",
      "author": "Goldstein",
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      "author": "Gorlich",
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      "volume": "129",
      "author": "Gorsch",
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      "journal-title": "J Cell Biol"
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      "first-page": "3061",
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      "volume": "12",
      "author": "Grandi",
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      "volume": "93",
      "author": "Hampton",
      "year": "1996",
      "journal-title": "Proc Natl Acad Sci USA"
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      "key": "2023072206424392900_B25",
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      "first-page": "1677",
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      "volume": "131",
      "author": "Heath",
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      "journal-title": "J Cell Biol"
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      "volume": "6",
      "author": "Heim",
      "year": "1996",
      "journal-title": "Curr Biol"
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      "volume": "131",
      "author": "Iovine",
      "year": "1995",
      "journal-title": "J Cell Biol"
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      "first-page": "163",
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      "article-title": "Transformation of intact yeast cells treated with alkali cations",
      "volume": "153",
      "author": "Ito",
      "year": "1983",
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      "volume": "104",
      "author": "Jordan",
      "year": "1977",
      "journal-title": "Exp Cell Res"
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      "volume": "92",
      "author": "Kahana",
      "year": "1995",
      "journal-title": "Proc Natl Acad Sci USA"
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      "volume": "82",
      "author": "Kessel",
      "year": "1983",
      "journal-title": "Int Rev Cytol"
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      "first-page": "911",
      "DOI": "10.1083/jcb.126.4.911",
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      "volume": "126",
      "author": "Kurihara",
      "year": "1994",
      "journal-title": "J Cell Biol"
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      "author": "Latterich",
      "year": "1994",
      "journal-title": "Cell"
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      "volume": "6",
      "author": "Li",
      "year": "1995",
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      "volume": "220",
      "author": "Lohka",
      "year": "1983",
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      "first-page": "5",
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      "volume": "132",
      "author": "Macaulay",
      "year": "1996",
      "journal-title": "J Cell Biol"
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      "article-title": "The nuclear and the cytoplasmic pore complex: structure, dynamics, distribution, and evolution",
      "volume": "5",
      "author": "Maul",
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      "first-page": "1459",
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      "volume": "129",
      "author": "Meier",
      "year": "1995",
      "journal-title": "J Cell Biol"
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      "author": "Melchior",
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      "volume": "60",
      "author": "Meluh",
      "year": "1990",
      "journal-title": "Cell"
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      "volume": "7",
      "author": "Murphy",
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      "article-title": "NSP1 depletion in yeast affects nuclear pore formation and nuclear accumulation",
      "volume": "59",
      "author": "Mutvei",
      "year": "1992",
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      "author": "Nehrbass",
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      "volume": "110",
      "author": "Newmeyer",
      "year": "1990",
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      "author": "Newport",
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      "author": "Newport",
      "year": "1992",
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      "first-page": "219",
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      "volume": "62",
      "author": "Osborne",
      "year": "1993",
      "journal-title": "Annu Rev Biochem"
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      "key": "2023072206424392900_B48",
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      "first-page": "153",
      "DOI": "10.3109/10409239609106583",
      "article-title": "Molecular dissection of the nuclear pore complex",
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      "author": "Pante",
      "year": "1996",
      "journal-title": "Crit Rev Biochem Mol Biol"
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      "first-page": "1187",
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      "volume": "92",
      "author": "Pemberton",
      "year": "1995",
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      "author": "Pfaller",
      "year": "1991",
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      "volume": "21",
      "author": "Ris",
      "year": "1991",
      "journal-title": "EMSA Bull"
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      "author": "Rose",
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      "author": "Rout",
      "year": "1993",
      "journal-title": "J Cell Biol"
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      "volume": "54",
      "author": "Severs",
      "year": "1976",
      "journal-title": "J Ultrastruct Res"
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      "first-page": "1",
      "DOI": "10.1083/jcb.106.1.1",
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      "volume": "106",
      "author": "Sheehan",
      "year": "1988",
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      "volume": "122",
      "author": "Sikorski",
      "year": "1989",
      "journal-title": "Genetics"
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      "author": "Siniossoglou",
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      "journal-title": "Cell"
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      "volume": "91",
      "author": "Smith",
      "year": "1994",
      "journal-title": "Proc Natl Acad Sci USA"
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      "article-title": "Green fluorescent protein. The green revolution",
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      "author": "Stearns",
      "year": "1995",
      "journal-title": "Curr Biol"
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      "author": "Stewart",
      "year": "1992",
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      "author": "Sullivan",
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      "volume": "9",
      "author": "Underwood",
      "year": "1990",
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      "author": "Vallen",
      "year": "1992",
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      "volume": "112",
      "author": "Vigers",
      "year": "1991",
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      "first-page": "273",
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      "author": "Vigers",
      "year": "1992",
      "journal-title": "J Cell Sci"
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      "first-page": "861",
      "DOI": "10.1083/jcb.132.5.861",
      "article-title": "Movement of cortical actin patches in yeast",
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      "author": "Waddle",
      "year": "1996",
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      "author": "Wente",
      "year": "1993",
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      "author": "Wente",
      "year": "1994",
      "journal-title": "J Cell Biol"
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      "author": "Wente",
      "year": "1992",
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      "author": "Wiese",
      "year": "1993",
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      "volume": "107",
      "author": "Wright",
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      "volume": "130",
      "author": "Yeh",
      "year": "1995",
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