DOI: 10.1083/jcb.136.3.649. Bee1, a Yeast Protein with Homology to Wiscott-Aldrich Syndrome Protein, Is Critical for the Assembly of Cortical Actin Cytoskeleton

Bee1, a Yeast Protein with Homology to Wiscott-Aldrich Syndrome Protein, Is Critical for the Assembly of Cortical Actin Cytoskeleton

10.1083/jcb.136.3.649

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Yeast protein, Bee1, exhibits sequence homology to Wiskott-Aldrich syndrome protein (WASP), a human protein that may link signaling pathways to the actin cytoskeleton. Mutations in WASP are the primary cause of Wiskott-Aldrich syndrome, characterized by immuno-deficiencies and defects in blood cell morphogenesis. This report describes the characterization of Bee1 protein function in budding yeast. Disruption of BEE1 causes a striking change in the organization of actin filaments, resulting in defects in budding and cytokinesis. Rather than assemble into cortically associated patches, actin filaments in the buds of Δbee1 cells form aberrant bundles that do not contain most of the cortical cytoskeletal components. It is significant that Δbee1 is the only mutation reported so far that abolishes cortical actin patches in the bud. Bee1 protein is localized to actin patches and interacts with Sla1p, a Src homology 3 domain–containing protein previously implicated in actin assembly and function. Thus, Bee1 protein may be a crucial component of a cytoskeletal complex that controls the assembly and organization of actin filaments at the cell cortex.

Bibliography

Li, R. (1997). Bee1, a Yeast Protein with Homology to Wiscott-Aldrich Syndrome Protein, Is Critical for the Assembly of Cortical Actin Cytoskeleton. The Journal of Cell Biology, 136(3), 649â658.

Authors 1

Rong Li (first)

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Dates

Type	When
Created	23 years ago (July 26, 2002, 12:47 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 2:46 a.m.)
Indexed	3 months, 1 week ago (May 16, 2025, 6:26 a.m.)
Issued	28 years, 6 months ago (Feb. 10, 1997)
Published	28 years, 6 months ago (Feb. 10, 1997)
Published Online	28 years, 6 months ago (Feb. 10, 1997)
Published Print	28 years, 6 months ago (Feb. 10, 1997)

Funders 0

None

BibTeX

@article{Li_1997, title={Bee1, a Yeast Protein with Homology to Wiscott-Aldrich Syndrome Protein, Is Critical for the Assembly of Cortical Actin Cytoskeleton}, volume={136}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.136.3.649}, DOI={10.1083/jcb.136.3.649}, number={3}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Li, Rong}, year={1997}, month=feb, pages={649–658} }

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