DOI: 10.1083/jcb.136.1.5. A Functional Interaction between the Carboxy-Terminal Domain of RNA Polymerase II and Pre-mRNA Splicing

A Functional Interaction between the Carboxy-Terminal Domain of RNA Polymerase II and Pre-mRNA Splicing

10.1083/jcb.136.1.5

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

In the preceding study we found that Sm snRNPs and SerArg (SR) family proteins co-immunoprecipitate with Pol II molecules containing a hyperphosphorylated CTD (Kim et al., 1997). The association between Pol IIo and splicing factors is maintained in the absence of pre-mRNA, and the polymerase need not be transcriptionally engaged (Kim et al., 1997). The latter findings led us to hypothesize that a phosphorylated form of the CTD interacts with pre-mRNA splicing components in vivo. To test this idea, a nested set of CTD-derived proteins was assayed for the ability to alter the nuclear distribution of splicing factors, and to interfere with splicing in vivo. Proteins containing heptapeptides 1-52 (CTD52), 1-32 (CTD32), 1-26 (CTD26), 1-13 (CTD13), 1-6 (CTD6), 1-3 (CTD3), or 1 (CTD1) were expressed in mammalian cells. The CTD-derived proteins become phosphorylated in vivo, and accumulate in the nucleus even though they lack a conventional nuclear localization signal. CTD52 induces a selective reorganization of splicing factors from discrete nuclear domains to the diffuse nucleoplasm, and significantly, it blocks the accumulation of spliced, but not unspliced, human β-globin transcripts. The extent of splicing factor disruption, and the degree of inhibition of splicing, are proportional to the number of heptapeptides added to the protein. The above results indicate a functional interaction between Pol II's CTD and pre-mRNA splicing.

Bibliography

Du, L., & Warren, S. L. (1997). A Functional Interaction between the Carboxy-Terminal Domain of RNA Polymerase II and Pre-mRNA Splicing. The Journal of Cell Biology, 136(1), 5â18.

Authors 2

Lei Du (first)
Stephen L. Warren (additional)

References 38 Referenced 107

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Dates

Type	When
Created	23 years ago (July 26, 2002, 12:46 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 2:39 a.m.)
Indexed	1 month, 3 weeks ago (July 2, 2025, 2:31 p.m.)
Issued	28 years, 7 months ago (Jan. 13, 1997)
Published	28 years, 7 months ago (Jan. 13, 1997)
Published Online	28 years, 7 months ago (Jan. 13, 1997)
Published Print	28 years, 7 months ago (Jan. 13, 1997)

Funders 0

None

BibTeX

@article{Du_1997, title={A Functional Interaction between the Carboxy-Terminal Domain of RNA Polymerase II and Pre-mRNA Splicing}, volume={136}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.136.1.5}, DOI={10.1083/jcb.136.1.5}, number={1}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Du, Lei and Warren, Stephen L.}, year={1997}, month=jan, pages={5–18} }

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