DOI: 10.1083/jcb.134.2.269. Signal sequences specify the targeting route to the endoplasmic reticulum membrane.

Signal sequences specify the targeting route to the endoplasmic reticulum membrane.

10.1083/jcb.134.2.269

Crossref journal-article

Rockefeller University Press

The Journal of cell biology (291)

Abstract

In the yeast Saccharomyces cerevisiae, only a subset of preproteins that are translocated across the ER membrane require the function of the signal recognition particle (SRP), suggesting that an alternative, SRP-independent pathway must exist (Hann, B.C., and P. Walter. 1991. Cell. 67:131-144). We have established that the two targeting pathways function in parallel. Mutant alleles of SEC62 and SEC63 were isolated that specifically impaired the translocation of SRP-independent preproteins in vivo and in vitro, whereas SRP-dependent preproteins were unaffected. Based on this analysis, preproteins fall into three distinct classes: SRP dependent, SRP independent, and those that can use both pathways. Pathway specificity is conferred by the hydrophobic core of signal sequences. Our studies show a previously unrecognized diversity in ER-directed signal sequences, that carry structural information that serves to identify the route taken.

Bibliography

Ng, D. T., Brown, J. D., & Walter, P. (1996). Signal sequences specify the targeting route to the endoplasmic reticulum membrane. The Journal of Cell Biology, 134(2), 269â278.

Authors 3

D T Ng (first)
J D Brown (additional)
P Walter (additional)

References 0 Referenced 380

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 9:23 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 2:25 a.m.)
Indexed	3 weeks, 2 days ago (Aug. 3, 2025, 6:52 p.m.)
Issued	29 years, 1 month ago (July 15, 1996)
Published	29 years, 1 month ago (July 15, 1996)
Published Online	29 years, 1 month ago (July 15, 1996)
Published Print	29 years, 1 month ago (July 15, 1996)

Funders 0

None

BibTeX

@article{Ng_1996, title={Signal sequences specify the targeting route to the endoplasmic reticulum membrane.}, volume={134}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.134.2.269}, DOI={10.1083/jcb.134.2.269}, number={2}, journal={The Journal of cell biology}, publisher={Rockefeller University Press}, author={Ng, D T and Brown, J D and Walter, P}, year={1996}, month=jul, pages={269–278} }

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