DOI: 10.1083/jcb.126.4.853. Yeast NPI46 encodes a novel prolyl cis-trans isomerase that is located in the nucleolus.

Yeast NPI46 encodes a novel prolyl cis-trans isomerase that is located in the nucleolus.

10.1083/jcb.126.4.853

Crossref journal-article

Rockefeller University Press

The Journal of cell biology (291)

Abstract

We have identified a gene (NPI46) encoding a new prolyl cis-trans isomerase within the nucleolus of the yeast Saccharomyces cerevisiae. The protein encoded by NPI46 was originally found by us in a search for proteins that recognize nuclear localization sequences (NLSs) in vitro. Thus, NPI46 binds to affinity columns that contain a wild-type histone H2B NLS but not a mutant H2B NLS that is incompetent for nuclear localization in vivo. NPI46 has two domains, a highly charged NH2 terminus similar to two other mammalian nucleolar proteins, nucleolin and Nopp140, and a COOH terminus with 45% homology to a family of mammalian and yeast proline isomerases. NPI46 is capable of catalyzing the prolyl cis-trans isomerization of two small synthetic peptides, succinyl-Ala-Leu-Pro-Phe-p-nitroanilide and succinyl-Ala-Ala-Pro-Phe-p-nitroanilide, as measured by a chymotrypsin-coupled spectrophotometric assay. By indirect immunofluorescence we have shown that NPI46 is a nucleolar protein. NPI46 is not essential for cell viability.

Bibliography

Shan, X., Xue, Z., & MÃ©lÃ¨se, T. (1994). Yeast NPI46 encodes a novel prolyl cis-trans isomerase that is located in the nucleolus. The Journal of Cell Biology, 126(4), 853â862.

Authors 3

X Shan (first)
Z Xue (additional)
T Mélèse (additional)

References 0 Referenced 49

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 8:22 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 1:36 a.m.)
Indexed	1 year, 2 months ago (June 6, 2024, 5:14 p.m.)
Issued	31 years ago (Aug. 15, 1994)
Published	31 years ago (Aug. 15, 1994)
Published Online	31 years ago (Aug. 15, 1994)
Published Print	31 years ago (Aug. 15, 1994)

Funders 0

None

BibTeX

@article{Shan_1994, title={Yeast NPI46 encodes a novel prolyl cis-trans isomerase that is located in the nucleolus.}, volume={126}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.126.4.853}, DOI={10.1083/jcb.126.4.853}, number={4}, journal={The Journal of cell biology}, publisher={Rockefeller University Press}, author={Shan, X and Xue, Z and Mélèse, T}, year={1994}, month=aug, pages={853–862} }

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