DOI: 10.1083/jcb.126.4.1079. Production of hydrogen peroxide by transforming growth factor-beta 1 and its involvement in induction of egr-1 in mouse osteoblastic cells.

Production of hydrogen peroxide by transforming growth factor-beta 1 and its involvement in induction of egr-1 in mouse osteoblastic cells.

10.1083/jcb.126.4.1079

Crossref journal-article

Rockefeller University Press

The Journal of cell biology (291)

Abstract

TGF-beta 1 controls the expression of numerous genes, including early response and cellular matrix genes. However, the signal-transducing mechanism underlying this regulation of gene expression is not fully understood. In this study, we investigated whether redox regulation plays a role in the TGF-beta 1 signal transduction in the mouse osteoblastic cell line (MC3T3-E1). The overall intracellular oxidized state of the cells, when measured using 2',7'-dichlorofluorescin diacetate by laser-scanning confocal microscopy, was increased transiently after the addition of TGF-beta 1. This increase was abolished by the addition of oxygen radical scavengers such as catalase and N-acetylcysteine. In a variant cell line lacking the TGF-beta 1 receptor, the intracellular oxidized state was not modulated by treatment with TGF-beta 1. We then examined the expression of early growth response-1 (egr-1) gene, which is inducible by TGF-beta 1 and H2O2. Radical scavengers inhibited the induction of egr-1 by TGF-beta 1, but not that by 12-O-tetradecanoylphorbol-13 acetate. A nuclear run-on assay indicated that this inhibition was at the transcriptional level. From transient expression experiments using chloramphenicol acetyltransferase gene linked to serially deleted egr-1 gene 5'-upstream region, the CArG element in the 5' flanking region of egr-1 was identified as an essential sequence in the transcriptional activation for both TGF-beta 1 and H2O2 stimulation. These findings suggest that H2O2 acts as a mediator for the TGF-beta 1-induced transcription of egr-1 gene.

Bibliography

Ohba, M., Shibanuma, M., Kuroki, T., & Nose, K. (1994). Production of hydrogen peroxide by transforming growth factor-beta 1 and its involvement in induction of egr-1 in mouse osteoblastic cells. The Journal of Cell Biology, 126(4), 1079â1088.

Authors 4

M Ohba (first)
M Shibanuma (additional)
T Kuroki (additional)
K Nose (additional)

References 0 Referenced 384

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 8:22 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 1:34 a.m.)
Indexed	1 month, 3 weeks ago (July 7, 2025, 1:45 a.m.)
Issued	31 years ago (Aug. 15, 1994)
Published	31 years ago (Aug. 15, 1994)
Published Online	31 years ago (Aug. 15, 1994)
Published Print	31 years ago (Aug. 15, 1994)

Funders 0

None

BibTeX

@article{Ohba_1994, title={Production of hydrogen peroxide by transforming growth factor-beta 1 and its involvement in induction of egr-1 in mouse osteoblastic cells.}, volume={126}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.126.4.1079}, DOI={10.1083/jcb.126.4.1079}, number={4}, journal={The Journal of cell biology}, publisher={Rockefeller University Press}, author={Ohba, M and Shibanuma, M and Kuroki, T and Nose, K}, year={1994}, month=aug, pages={1079–1088} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        7,
        7
      ]
    ],
    "date-time": "2025-07-07T05:45:24Z",
    "timestamp": 1751867124333
  },
  "reference-count": 0,
  "publisher": "Rockefeller University Press",
  "issue": "4",
  "content-domain": {
    "domain": [
      "rupress.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1994,
        8,
        15
      ]
    ]
  },
  "abstract": "<jats:p>TGF-beta 1 controls the expression of numerous genes, including early response and cellular matrix genes. However, the signal-transducing mechanism underlying this regulation of gene expression is not fully understood. In this study, we investigated whether redox regulation plays a role in the TGF-beta 1 signal transduction in the mouse osteoblastic cell line (MC3T3-E1). The overall intracellular oxidized state of the cells, when measured using 2',7'-dichlorofluorescin diacetate by laser-scanning confocal microscopy, was increased transiently after the addition of TGF-beta 1. This increase was abolished by the addition of oxygen radical scavengers such as catalase and N-acetylcysteine. In a variant cell line lacking the TGF-beta 1 receptor, the intracellular oxidized state was not modulated by treatment with TGF-beta 1. We then examined the expression of early growth response-1 (egr-1) gene, which is inducible by TGF-beta 1 and H2O2. Radical scavengers inhibited the induction of egr-1 by TGF-beta 1, but not that by 12-O-tetradecanoylphorbol-13 acetate. A nuclear run-on assay indicated that this inhibition was at the transcriptional level. From transient expression experiments using chloramphenicol acetyltransferase gene linked to serially deleted egr-1 gene 5'-upstream region, the CArG element in the 5' flanking region of egr-1 was identified as an essential sequence in the transcriptional activation for both TGF-beta 1 and H2O2 stimulation. These findings suggest that H2O2 acts as a mediator for the TGF-beta 1-induced transcription of egr-1 gene.</jats:p>",
  "DOI": "10.1083/jcb.126.4.1079",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2004,
        5,
        15
      ]
    ],
    "date-time": "2004-05-15T00:22:22Z",
    "timestamp": 1084580542000
  },
  "page": "1079-1088",
  "update-policy": "http://dx.doi.org/10.1083/jcb.crossmarkpolicy",
  "source": "Crossref",
  "is-referenced-by-count": 384,
  "title": "Production of hydrogen peroxide by transforming growth factor-beta 1 and its involvement in induction of egr-1 in mouse osteoblastic cells.",
  "prefix": "10.1083",
  "volume": "126",
  "author": [
    {
      "given": "M",
      "family": "Ohba",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Department of Cancer Cell Research, Institute of Medical Science, University of Tokyo, Japan."
        }
      ]
    },
    {
      "given": "M",
      "family": "Shibanuma",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Cancer Cell Research, Institute of Medical Science, University of Tokyo, Japan."
        }
      ]
    },
    {
      "given": "T",
      "family": "Kuroki",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Cancer Cell Research, Institute of Medical Science, University of Tokyo, Japan."
        }
      ]
    },
    {
      "given": "K",
      "family": "Nose",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Cancer Cell Research, Institute of Medical Science, University of Tokyo, Japan."
        }
      ]
    }
  ],
  "member": "291",
  "published-online": {
    "date-parts": [
      [
        1994,
        8,
        15
      ]
    ]
  },
  "container-title": "The Journal of cell biology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://rupress.org/jcb/article-pdf/126/4/1079/1477438/1079.pdf",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "syndication"
    },
    {
      "URL": "https://rupress.org/jcb/article-pdf/126/4/1079/1477438/1079.pdf",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        7,
        22
      ]
    ],
    "date-time": "2023-07-22T05:34:54Z",
    "timestamp": 1690004094000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://rupress.org/jcb/article/126/4/1079/14970/Production-of-hydrogen-peroxide-by-transforming"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1994,
        8,
        15
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "4",
    "published-print": {
      "date-parts": [
        [
          1994,
          8,
          15
        ]
      ]
    }
  },
  "URL": "http://dx.doi.org/10.1083/jcb.126.4.1079",
  "relation": {},
  "ISSN": [
    "0021-9525",
    "1540-8140"
  ],
  "subject": [],
  "published": {
    "date-parts": [
      [
        1994,
        8,
        15
      ]
    ]
  }
}