DOI: 10.1083/jcb.125.4.843. Direct interaction between yeast spindle pole body components: Kar1p is required for Cdc31p localization to the spindle pole body.

Direct interaction between yeast spindle pole body components: Kar1p is required for Cdc31p localization to the spindle pole body.

10.1083/jcb.125.4.843

Crossref journal-article

Rockefeller University Press

The Journal of cell biology (291)

Abstract

The Saccharomyces cerevisiae genes KAR1 and CDC31 are required for the initial stages of spindle pole body (SPB) duplication in yeast. The Cdc31 protein is most related to caltractin/centrin, a calcium-binding protein present in microtubule organizing centers in many organisms. Because of a variety of genetic interactions between CDC31 and KAR1 (Vallen, E. A., W. Ho. M. Winey, and M. D. Rose. 1994. Genetics. In press), we wanted to determine whether Cdc31p and Kar1p physically interact. Cdc31p was expressed and purified from Escherichia coli and active for binding calcium. Using a protein blotting technique, Cdc31p bound to Kar1p in vitro via an essential domain in Kar1p required for SPB duplication (Vallen, E. A., M. A. Hiller, T. Y. Scherson, and M. D. Rose. 1992a. J. Cell Biol. 117:1277-1287). By immunofluorescence microscopy, we determined that the interaction also occurs in vivo. Cdc31p was localized to the SPB in wild-type cells but was mislocalized in a kar1 mutant strain. In a kar1 mutant containing a dominant CDC31 suppressor, Cdc31p was again localized to the SPB. Furthermore, the localization of Cdc31p to the SPB was affected by the overexpression of Kar1p-beta-galactosidase hybrids. Based on these data, we propose that the essential function of Kar1p is to localize Cdc31p to the SPB, and that this interaction is normally required for SPB duplication.

Bibliography

Biggins, S., & Rose, M. D. (1994). Direct interaction between yeast spindle pole body components: Kar1p is required for Cdc31p localization to the spindle pole body. The Journal of Cell Biology, 125(4), 843â852.

Authors 2

S Biggins (first)
M D Rose (additional)

References 0 Referenced 74

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 8:22 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 12:45 a.m.)
Indexed	2 months, 3 weeks ago (June 10, 2025, 1:48 p.m.)
Issued	31 years, 3 months ago (May 15, 1994)
Published	31 years, 3 months ago (May 15, 1994)
Published Online	31 years, 3 months ago (May 15, 1994)
Published Print	31 years, 3 months ago (May 15, 1994)

Funders 0

None

BibTeX

@article{Biggins_1994, title={Direct interaction between yeast spindle pole body components: Kar1p is required for Cdc31p localization to the spindle pole body.}, volume={125}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.125.4.843}, DOI={10.1083/jcb.125.4.843}, number={4}, journal={The Journal of cell biology}, publisher={Rockefeller University Press}, author={Biggins, S and Rose, M D}, year={1994}, month=may, pages={843–852} }

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