DOI: 10.1083/jcb.125.3.547. Identification of cytosolic factors required for nuclear location sequence-mediated binding to the nuclear envelope.

Identification of cytosolic factors required for nuclear location sequence-mediated binding to the nuclear envelope.

10.1083/jcb.125.3.547

Crossref journal-article

Rockefeller University Press

The Journal of cell biology (291)

Abstract

Nuclear protein import can be separated into two distinct steps: binding to the nuclear pore complex followed by translocation to the nuclear interior. A previously identified nuclear location sequence (NLS) receptor and a 97-kD protein purified from bovine erythrocytes reconstitute the binding step in a permeabilized cell assay. Binding to the envelope is specific for a functional SV-40 large T antigen NLS and is not ATP or temperature dependent. Modification of p97 with N-ethylmaleimide (NEM) decreases binding to the pore, but interestingly, NEM treatment of the NLS receptor does not. Nuclear envelope binding is inhibited by wheat germ agglutinin suggesting a possible mechanism for the inhibition of transport by the lectin.

Bibliography

Adam, E. J., & Adam, S. A. (1994). Identification of cytosolic factors required for nuclear location sequence-mediated binding to the nuclear envelope. The Journal of Cell Biology, 125(3), 547â555.

Authors 2

E J Adam (first)
S A Adam (additional)

References 0 Referenced 211

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 8:22 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 12:42 a.m.)
Indexed	1 month, 3 weeks ago (July 2, 2025, 2:45 p.m.)
Issued	31 years, 3 months ago (May 1, 1994)
Published	31 years, 3 months ago (May 1, 1994)
Published Online	31 years, 3 months ago (May 1, 1994)
Published Print	31 years, 3 months ago (May 1, 1994)

Funders 0

None

BibTeX

@article{Adam_1994, title={Identification of cytosolic factors required for nuclear location sequence-mediated binding to the nuclear envelope.}, volume={125}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.125.3.547}, DOI={10.1083/jcb.125.3.547}, number={3}, journal={The Journal of cell biology}, publisher={Rockefeller University Press}, author={Adam, E J and Adam, S A}, year={1994}, month=may, pages={547–555} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        7,
        2
      ]
    ],
    "date-time": "2025-07-02T18:45:51Z",
    "timestamp": 1751481951510
  },
  "reference-count": 0,
  "publisher": "Rockefeller University Press",
  "issue": "3",
  "content-domain": {
    "domain": [
      "rupress.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1994,
        5,
        1
      ]
    ]
  },
  "abstract": "<jats:p>Nuclear protein import can be separated into two distinct steps: binding to the nuclear pore complex followed by translocation to the nuclear interior. A previously identified nuclear location sequence (NLS) receptor and a 97-kD protein purified from bovine erythrocytes reconstitute the binding step in a permeabilized cell assay. Binding to the envelope is specific for a functional SV-40 large T antigen NLS and is not ATP or temperature dependent. Modification of p97 with N-ethylmaleimide (NEM) decreases binding to the pore, but interestingly, NEM treatment of the NLS receptor does not. Nuclear envelope binding is inhibited by wheat germ agglutinin suggesting a possible mechanism for the inhibition of transport by the lectin.</jats:p>",
  "DOI": "10.1083/jcb.125.3.547",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2004,
        5,
        15
      ]
    ],
    "date-time": "2004-05-15T00:22:22Z",
    "timestamp": 1084580542000
  },
  "page": "547-555",
  "update-policy": "http://dx.doi.org/10.1083/jcb.crossmarkpolicy",
  "source": "Crossref",
  "is-referenced-by-count": 211,
  "title": "Identification of cytosolic factors required for nuclear location sequence-mediated binding to the nuclear envelope.",
  "prefix": "10.1083",
  "volume": "125",
  "author": [
    {
      "given": "E J",
      "family": "Adam",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Department of Cell, Molecular and Structural Biology, Northwestern University Medical School, Chicago, Illinois 60611."
        }
      ]
    },
    {
      "given": "S A",
      "family": "Adam",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Cell, Molecular and Structural Biology, Northwestern University Medical School, Chicago, Illinois 60611."
        }
      ]
    }
  ],
  "member": "291",
  "published-online": {
    "date-parts": [
      [
        1994,
        5,
        1
      ]
    ]
  },
  "container-title": "The Journal of cell biology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://rupress.org/jcb/article-pdf/125/3/547/1475695/547.pdf",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "syndication"
    },
    {
      "URL": "https://rupress.org/jcb/article-pdf/125/3/547/1475695/547.pdf",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        7,
        22
      ]
    ],
    "date-time": "2023-07-22T04:42:32Z",
    "timestamp": 1690000952000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://rupress.org/jcb/article/125/3/547/59529/Identification-of-cytosolic-factors-required-for"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1994,
        5,
        1
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "3",
    "published-print": {
      "date-parts": [
        [
          1994,
          5,
          1
        ]
      ]
    }
  },
  "URL": "http://dx.doi.org/10.1083/jcb.125.3.547",
  "relation": {},
  "ISSN": [
    "0021-9525",
    "1540-8140"
  ],
  "subject": [],
  "published": {
    "date-parts": [
      [
        1994,
        5,
        1
      ]
    ]
  }
}