DOI: 10.1083/jcb.124.3.307. Protein kinase C activators inhibit receptor-mediated potocytosis by preventing internalization of caveolae

Protein kinase C activators inhibit receptor-mediated potocytosis by preventing internalization of caveolae

10.1083/jcb.124.3.307

Crossref journal-article

Rockefeller University Press

The Journal of cell biology (291)

Abstract

Potocytosis is an endocytic pathway that utilizes glycosylphosphatidylinositol-anchored membrane proteins and caveolae to concentrate and internalize small molecules. We now report that activators of protein kinase C are potent inhibitors of potocytosis. Activators such as phorbol-12-myristate-13-acetate (PMA) inhibit the internalization of receptors for 5-methyltetrahydrofolate but allow the internal receptor pool to return to the cell surface. PMA does not affect the clustering of the folate receptor but instead markedly reduces the number of caveolae. Exposure to PMA totally blocks the intracellular accumulation of 5-methyltetrahydrofolate without affecting receptor-independent uptake or the formation of polyglutamylated species of 5-methyltetrahydrofolate in the cytoplasm. These data suggest that PMA inhibits uptake by inactivating caveolae internalization.

Bibliography

Smart, E., Foster, D., Ying, Y., Kamen, B., & Anderson, R. (1994). Protein kinase C activators inhibit receptor-mediated potocytosis by preventing internalization of caveolae. The Journal of Cell Biology, 124(3), 307â313.

Authors 5

EJ Smart (first)
DC Foster (additional)
YS Ying (additional)
BA Kamen (additional)
RG Anderson (additional)

References 0 Referenced 144

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 8:22 p.m.)
Deposited	2 years ago (July 22, 2023, 12:36 a.m.)
Indexed	2 weeks ago (Aug. 7, 2025, 4:53 a.m.)
Issued	31 years, 6 months ago (Feb. 1, 1994)
Published	31 years, 6 months ago (Feb. 1, 1994)
Published Online	31 years, 6 months ago (Feb. 1, 1994)
Published Print	31 years, 6 months ago (Feb. 1, 1994)

Funders 0

None

BibTeX

@article{Smart_1994, title={Protein kinase C activators inhibit receptor-mediated potocytosis by preventing internalization of caveolae}, volume={124}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.124.3.307}, DOI={10.1083/jcb.124.3.307}, number={3}, journal={The Journal of cell biology}, publisher={Rockefeller University Press}, author={Smart, EJ and Foster, DC and Ying, YS and Kamen, BA and Anderson, RG}, year={1994}, month=feb, pages={307–313} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        7
      ]
    ],
    "date-time": "2025-08-07T08:53:27Z",
    "timestamp": 1754556807981
  },
  "reference-count": 0,
  "publisher": "Rockefeller University Press",
  "issue": "3",
  "content-domain": {
    "domain": [
      "rupress.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1994,
        2,
        1
      ]
    ]
  },
  "abstract": "<jats:p>Potocytosis is an endocytic pathway that utilizes glycosylphosphatidylinositol-anchored membrane proteins and caveolae to concentrate and internalize small molecules. We now report that activators of protein kinase C are potent inhibitors of potocytosis. Activators such as phorbol-12-myristate-13-acetate (PMA) inhibit the internalization of receptors for 5-methyltetrahydrofolate but allow the internal receptor pool to return to the cell surface. PMA does not affect the clustering of the folate receptor but instead markedly reduces the number of caveolae. Exposure to PMA totally blocks the intracellular accumulation of 5-methyltetrahydrofolate without affecting receptor-independent uptake or the formation of polyglutamylated species of 5-methyltetrahydrofolate in the cytoplasm. These data suggest that PMA inhibits uptake by inactivating caveolae internalization.</jats:p>",
  "DOI": "10.1083/jcb.124.3.307",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2004,
        5,
        15
      ]
    ],
    "date-time": "2004-05-15T00:22:22Z",
    "timestamp": 1084580542000
  },
  "page": "307-313",
  "update-policy": "http://dx.doi.org/10.1083/jcb.crossmarkpolicy",
  "source": "Crossref",
  "is-referenced-by-count": 144,
  "title": "Protein kinase C activators inhibit receptor-mediated potocytosis by preventing internalization of caveolae",
  "prefix": "10.1083",
  "volume": "124",
  "author": [
    {
      "given": "EJ",
      "family": "Smart",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Department of Cell Biology and Neuroscience, University of Texas Southwestern Medical Center, Dallas 75235."
        }
      ]
    },
    {
      "given": "DC",
      "family": "Foster",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Cell Biology and Neuroscience, University of Texas Southwestern Medical Center, Dallas 75235."
        }
      ]
    },
    {
      "given": "YS",
      "family": "Ying",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Cell Biology and Neuroscience, University of Texas Southwestern Medical Center, Dallas 75235."
        }
      ]
    },
    {
      "given": "BA",
      "family": "Kamen",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Cell Biology and Neuroscience, University of Texas Southwestern Medical Center, Dallas 75235."
        }
      ]
    },
    {
      "given": "RG",
      "family": "Anderson",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Cell Biology and Neuroscience, University of Texas Southwestern Medical Center, Dallas 75235."
        }
      ]
    }
  ],
  "member": "291",
  "published-online": {
    "date-parts": [
      [
        1994,
        2,
        1
      ]
    ]
  },
  "container-title": "The Journal of cell biology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://rupress.org/jcb/article-pdf/124/3/307/1475128/307.pdf",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "syndication"
    },
    {
      "URL": "https://rupress.org/jcb/article-pdf/124/3/307/1475128/307.pdf",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        7,
        22
      ]
    ],
    "date-time": "2023-07-22T04:36:51Z",
    "timestamp": 1690000611000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://rupress.org/jcb/article/124/3/307/28767/Protein-kinase-C-activators-inhibit-receptor"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1994,
        2,
        1
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "3",
    "published-print": {
      "date-parts": [
        [
          1994,
          2,
          1
        ]
      ]
    }
  },
  "URL": "http://dx.doi.org/10.1083/jcb.124.3.307",
  "relation": {},
  "ISSN": [
    "0021-9525",
    "1540-8140"
  ],
  "subject": [],
  "published": {
    "date-parts": [
      [
        1994,
        2,
        1
      ]
    ]
  }
}