DOI: 10.1083/jcb.124.3.273. A phosphatidylinositol transfer protein controls the phosphatidylcholine content of yeast Golgi membranes

A phosphatidylinositol transfer protein controls the phosphatidylcholine content of yeast Golgi membranes

10.1083/jcb.124.3.273

Crossref journal-article

Rockefeller University Press

The Journal of cell biology (291)

Abstract

SEC14p is required for protein transport from the yeast Golgi complex. We describe a quantitative analysis of yeast bulk membrane and Golgi membrane phospholipid composition under conditions where Golgi secretory function has been uncoupled from its usual SEC14p requirement. The data demonstrate that SEC14p specifically functions to maintain a reduced phosphatidylcholine content in Golgi membranes and indicate that overproduction of SEC14p markedly reduces the apparent rate of phosphatidylcholine biosynthesis via the CDP-choline pathway in vivo. We suggest that SEC14p serves as a sensor of Golgi membrane phospholipid composition through which the activity of the CDP-choline pathway in Golgi membranes is regulated such that a phosphatidylcholine content that is compatible with the essential secretory function of these membranes is maintained.

Bibliography

McGee, T., Skinner, H., Whitters, E., Henry, S., & Bankaitis, V. (1994). A phosphatidylinositol transfer protein controls the phosphatidylcholine content of yeast Golgi membranes. The Journal of Cell Biology, 124(3), 273â287.

Authors 5

TP McGee (first)
HB Skinner (additional)
EA Whitters (additional)
SA Henry (additional)
VA Bankaitis (additional)

References 0 Referenced 140

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 8:22 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 12:36 a.m.)
Indexed	4 weeks ago (July 26, 2025, 4:42 a.m.)
Issued	31 years, 6 months ago (Feb. 1, 1994)
Published	31 years, 6 months ago (Feb. 1, 1994)
Published Online	31 years, 6 months ago (Feb. 1, 1994)
Published Print	31 years, 6 months ago (Feb. 1, 1994)

Funders 0

None

BibTeX

@article{McGee_1994, title={A phosphatidylinositol transfer protein controls the phosphatidylcholine content of yeast Golgi membranes}, volume={124}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.124.3.273}, DOI={10.1083/jcb.124.3.273}, number={3}, journal={The Journal of cell biology}, publisher={Rockefeller University Press}, author={McGee, TP and Skinner, HB and Whitters, EA and Henry, SA and Bankaitis, VA}, year={1994}, month=feb, pages={273–287} }

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