DOI: 10.1083/jcb.123.6.1857. Expression of Wnt-1 in PC12 cells results in modulation of plakoglobin and E-cadherin and increased cellular adhesion.

Expression of Wnt-1 in PC12 cells results in modulation of plakoglobin and E-cadherin and increased cellular adhesion.

10.1083/jcb.123.6.1857

Crossref journal-article

Rockefeller University Press

The Journal of cell biology (291)

Abstract

The Wnt-1 gene plays an essential role in fetal brain development and encodes a secreted protein whose signaling mechanism is presently unknown. In this report we have investigated intracellular mechanisms by which the Wnt-1 gene induces morphological changes in PC12 pheochromocytoma cells. PC12 cells expressing Wnt-1 show increased steady-state levels of the adhesive junction protein plakoglobin, and an altered distribution of this protein within the cell. This effect appears similar to a modulation of the plakoglobin homolog, Armadillo, that occurs in Drosophila embryos in response to the Wnt-1 homolog, wingless (Riggleman, B., P. Schedl, and E. Wieschaus. 1990. Cell. 63:549-560). In addition, PC12/Wnt-1 cells show elevated expression of E-cadherin and increased calcium-dependent cell-cell adhesion. These results imply evolutionary conservation of cellular responses to Wnt-1/wingless and indicate that in certain cell types Wnt-1 may act to modulate cell adhesion mechanisms.

Authors 3

R S Bradley (first)
P Cowin (additional)
A M Brown (additional)

References 0 Referenced 191

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 8:22 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 2:11 a.m.)
Indexed	11 hours, 5 minutes ago (Sept. 3, 2025, 6:48 a.m.)
Issued	31 years, 8 months ago (Dec. 15, 1993)
Published	31 years, 8 months ago (Dec. 15, 1993)
Published Online	31 years, 8 months ago (Dec. 15, 1993)
Published Print	31 years, 8 months ago (Dec. 15, 1993)

Funders 0

None

BibTeX

@article{Bradley_1993, title={Expression of Wnt-1 in PC12 cells results in modulation of plakoglobin and E-cadherin and increased cellular adhesion.}, volume={123}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.123.6.1857}, DOI={10.1083/jcb.123.6.1857}, number={6}, journal={The Journal of cell biology}, publisher={Rockefeller University Press}, author={Bradley, R S and Cowin, P and Brown, A M}, year={1993}, month=dec, pages={1857–1865} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        9,
        3
      ]
    ],
    "date-time": "2025-09-03T10:48:17Z",
    "timestamp": 1756896497614
  },
  "reference-count": 0,
  "publisher": "Rockefeller University Press",
  "issue": "6",
  "content-domain": {
    "domain": [
      "rupress.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1993,
        12,
        15
      ]
    ]
  },
  "abstract": "<jats:p>The Wnt-1 gene plays an essential role in fetal brain development and encodes a secreted protein whose signaling mechanism is presently unknown. In this report we have investigated intracellular mechanisms by which the Wnt-1 gene induces morphological changes in PC12 pheochromocytoma cells. PC12 cells expressing Wnt-1 show increased steady-state levels of the adhesive junction protein plakoglobin, and an altered distribution of this protein within the cell. This effect appears similar to a modulation of the plakoglobin homolog, Armadillo, that occurs in Drosophila embryos in response to the Wnt-1 homolog, wingless (Riggleman, B., P. Schedl, and E. Wieschaus. 1990. Cell. 63:549-560). In addition, PC12/Wnt-1 cells show elevated expression of E-cadherin and increased calcium-dependent cell-cell adhesion. These results imply evolutionary conservation of cellular responses to Wnt-1/wingless and indicate that in certain cell types Wnt-1 may act to modulate cell adhesion mechanisms.</jats:p>",
  "DOI": "10.1083/jcb.123.6.1857",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2004,
        5,
        15
      ]
    ],
    "date-time": "2004-05-15T00:22:22Z",
    "timestamp": 1084580542000
  },
  "page": "1857-1865",
  "update-policy": "http://dx.doi.org/10.1083/jcb.crossmarkpolicy",
  "source": "Crossref",
  "is-referenced-by-count": 191,
  "title": "Expression of Wnt-1 in PC12 cells results in modulation of plakoglobin and E-cadherin and increased cellular adhesion.",
  "prefix": "10.1083",
  "volume": "123",
  "author": [
    {
      "given": "R S",
      "family": "Bradley",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Department of Cell Biology and Anatomy, Cornell University Medical College, New York, New York 10021."
        }
      ]
    },
    {
      "given": "P",
      "family": "Cowin",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Cell Biology and Anatomy, Cornell University Medical College, New York, New York 10021."
        }
      ]
    },
    {
      "given": "A M",
      "family": "Brown",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Cell Biology and Anatomy, Cornell University Medical College, New York, New York 10021."
        }
      ]
    }
  ],
  "member": "291",
  "published-online": {
    "date-parts": [
      [
        1993,
        12,
        15
      ]
    ]
  },
  "container-title": "The Journal of cell biology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://rupress.org/jcb/article-pdf/123/6/1857/1479574/1857.pdf",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "syndication"
    },
    {
      "URL": "https://rupress.org/jcb/article-pdf/123/6/1857/1479574/1857.pdf",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        7,
        22
      ]
    ],
    "date-time": "2023-07-22T06:11:47Z",
    "timestamp": 1690006307000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://rupress.org/jcb/article/123/6/1857/29375/Expression-of-Wnt-1-in-PC12-cells-results-in"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1993,
        12,
        15
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "6",
    "published-print": {
      "date-parts": [
        [
          1993,
          12,
          15
        ]
      ]
    }
  },
  "URL": "http://dx.doi.org/10.1083/jcb.123.6.1857",
  "relation": {},
  "ISSN": [
    "0021-9525",
    "1540-8140"
  ],
  "subject": [],
  "published": {
    "date-parts": [
      [
        1993,
        12,
        15
      ]
    ]
  }
}