DOI: 10.1083/jcb.123.2.477. Induction of a secondary body axis in Xenopus by antibodies to beta-catenin.

Induction of a secondary body axis in Xenopus by antibodies to beta-catenin.

10.1083/jcb.123.2.477

Crossref journal-article

Rockefeller University Press

The Journal of cell biology (291)

Abstract

We have obtained evidence that a known intracellular component of the cadherin cell-cell adhesion machinery, beta-catenin, contributes to the development of the body axis in the frog Xenopus laevis. Vertebrate beta-catenin is homologous to the Drosophila segment polarity gene product armadillo, and to vertebrate plakoglobin (McCrea, P. D., C. W. Turck, and B. Gumbiner. 1991. Science (Wash. DC). 254: 1359-1361.). Beta-Catenin was found present in all Xenopus embryonic stages examined, and associated with C-cadherin, the major cadherin present in early Xenopus embryos. To test beta-catenin's function, affinity purified Fab fragments were injected into ventral blastomeres of developing four-cell Xenopus embryos. A dramatic phenotype, the duplication of the dorsoanterior embryonic axis, was observed. Furthermore, Fab injections were capable of rescuing dorsal features in UV-ventralized embryos. Similar phenotypes have been observed in misexpression studies of the Wnt and other gene products, suggesting that beta-catenin participates in a signaling pathway which specifies embryonic patterning.

Bibliography

McCrea, P. D., Brieher, W. M., & Gumbiner, B. M. (1993). Induction of a secondary body axis in Xenopus by antibodies to beta-catenin. The Journal of Cell Biology, 123(2), 477â484.

Authors 3

P D McCrea (first)
W M Brieher (additional)
B M Gumbiner (additional)

References 0 Referenced 150

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 8:22 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 12:30 a.m.)
Indexed	1 year ago (Aug. 3, 2024, 4:56 p.m.)
Issued	31 years, 10 months ago (Oct. 15, 1993)
Published	31 years, 10 months ago (Oct. 15, 1993)
Published Online	31 years, 10 months ago (Oct. 15, 1993)
Published Print	31 years, 10 months ago (Oct. 15, 1993)

Funders 0

None

BibTeX

@article{McCrea_1993, title={Induction of a secondary body axis in Xenopus by antibodies to beta-catenin.}, volume={123}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.123.2.477}, DOI={10.1083/jcb.123.2.477}, number={2}, journal={The Journal of cell biology}, publisher={Rockefeller University Press}, author={McCrea, P D and Brieher, W M and Gumbiner, B M}, year={1993}, month=oct, pages={477–484} }

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