DOI: 10.1083/jcb.121.4.743. Sec61p is adjacent to nascent type I and type II signal-anchor proteins during their membrane insertion.

Sec61p is adjacent to nascent type I and type II signal-anchor proteins during their membrane insertion.

10.1083/jcb.121.4.743

Crossref journal-article

Rockefeller University Press

The Journal of cell biology (291)

Abstract

We have identified membrane components which are adjacent to type I and type II signal-anchor proteins during their insertion into the membrane of the ER. Using two different cross-linking approaches a 37-38-kD nonglycosylated protein, previously identified as P37 (High, S., D. Görlich, M. Wiedmann, T. A. Rapoport, and B. Dobberstein. 1991. J. Cell Biol. 113:35-44), was found adjacent to all the membrane inserted nascent chains used in this study. On the basis of immunoprecipitation, this ER protein was shown to be identical to the recently identified mammalian Sec61 protein. Thus, Sec61p is the principal cross-linking partner of both type I and type II signal-anchor proteins during their membrane insertion (this work), and of secretory proteins during their translocation (Görlich, D., S. Prehn, E. Hartmann, K.-U. Kalies, and T. A. Rapoport. 1992. Cell. 71:489-503). We propose that membrane proteins of both orientations, and secretory proteins employ the same ER translocation sites, and that Sec61p is a core component of these sites.

Bibliography

High, S., Andersen, S. S., GÃ¶rlich, D., Hartmann, E., Prehn, S., Rapoport, T. A., & Dobberstein, B. (1993). Sec61p is adjacent to nascent type I and type II signal-anchor proteins during their membrane insertion. The Journal of Cell Biology, 121(4), 743â750.

Authors 7

S High (first)
S S Andersen (additional)
D Görlich (additional)
E Hartmann (additional)
S Prehn (additional)
T A Rapoport (additional)
B Dobberstein (additional)

References 0 Referenced 84

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 8:22 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 12:21 a.m.)
Indexed	2 weeks, 4 days ago (Aug. 5, 2025, 8:38 a.m.)
Issued	32 years, 3 months ago (May 15, 1993)
Published	32 years, 3 months ago (May 15, 1993)
Published Online	32 years, 3 months ago (May 15, 1993)
Published Print	32 years, 3 months ago (May 15, 1993)

Funders 0

None

BibTeX

@article{High_1993, title={Sec61p is adjacent to nascent type I and type II signal-anchor proteins during their membrane insertion.}, volume={121}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.121.4.743}, DOI={10.1083/jcb.121.4.743}, number={4}, journal={The Journal of cell biology}, publisher={Rockefeller University Press}, author={High, S and Andersen, S S and Görlich, D and Hartmann, E and Prehn, S and Rapoport, T A and Dobberstein, B}, year={1993}, month=may, pages={743–750} }

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