DOI: 10.1083/jcb.121.3.513. Isolation and characterization of a gene (CBF2) specifying a protein component of the budding yeast kinetochore.

Isolation and characterization of a gene (CBF2) specifying a protein component of the budding yeast kinetochore.

10.1083/jcb.121.3.513

Crossref journal-article

Rockefeller University Press

The Journal of cell biology (291)

Abstract

We have cloned and determined the nucleotide sequence of the gene (CBF2) specifying the large (110 kD) subunit of the 240-kD multisubunit yeast centromere binding factor CBF3, which binds selectively in vitro to yeast centromere DNA and contains a minus end-directed microtubule motor activity. The deduced amino acid sequence of CBF2p shows no sequence homologies with known molecular motors, although a consensus nucleotide binding site is present. The CBF2 gene is essential for viability of yeast and is identical to NDC10, in which a conditional mutation leads to a defect in chromosome segregation (Goh, P.-Y., and J. V. Kilmartin, in this issue of The Journal of Cell Biology). The combined in vitro and in vivo evidence indicate that CBF2p is a key component of the budding yeast kinetochore.

Bibliography

Jiang, W., Lechner, J., & Carbon, J. (1993). Isolation and characterization of a gene (CBF2) specifying a protein component of the budding yeast kinetochore. The Journal of Cell Biology, 121(3), 513â519.

Authors 3

W Jiang (first)
J Lechner (additional)
J Carbon (additional)

References 0 Referenced 82

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 8:22 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 12:18 a.m.)
Indexed	1 month, 4 weeks ago (June 29, 2025, 3 a.m.)
Issued	32 years, 3 months ago (May 1, 1993)
Published	32 years, 3 months ago (May 1, 1993)
Published Online	32 years, 3 months ago (May 1, 1993)
Published Print	32 years, 3 months ago (May 1, 1993)

Funders 0

None

BibTeX

@article{Jiang_1993, title={Isolation and characterization of a gene (CBF2) specifying a protein component of the budding yeast kinetochore.}, volume={121}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.121.3.513}, DOI={10.1083/jcb.121.3.513}, number={3}, journal={The Journal of cell biology}, publisher={Rockefeller University Press}, author={Jiang, W and Lechner, J and Carbon, J}, year={1993}, month=may, pages={513–519} }

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