DOI: 10.1083/jcb.116.4.923. Identification and characterization of an actin-binding site of CapZ.

Identification and characterization of an actin-binding site of CapZ.

10.1083/jcb.116.4.923

Crossref journal-article

Rockefeller University Press

The Journal of cell biology (291)

Abstract

A mAb (1E5) that binds the COOH-terminal region of the beta subunit of chicken CapZ inhibits the ability of CapZ to bind the barbed ends of actin filaments and nucleate actin polymerization. CapZ prepared as fusion proteins in bacteria or nonfusion proteins by in vitro translation has activity similar to that of CapZ purified from muscle. Deletion of the COOH-terminus of the beta subunit of CapZ leads to a loss of CapZ's ability to bind the barbed ends of actin filaments. A peptide corresponding to the COOH-terminal region of CapZ beta, expressed as a fusion protein, binds actin monomers. The mAb 1E5 also inhibits the binding of this peptide to actin. These results suggest that the COOH-terminal region of the beta subunit of CapZ is an actin-binding site. The primary structure of this region is not similar to that of potential actin-binding sites identified in other proteins. In addition, the primary structure of this region is not conserved across species.

Bibliography

Hug, C., Miller, T. M., Torres, M. A., Casella, J. F., & Cooper, J. A. (1992). Identification and characterization of an actin-binding site of CapZ. The Journal of Cell Biology, 116(4), 923â931.

Authors 5

C Hug (first)
T M Miller (additional)
M A Torres (additional)
J F Casella (additional)
J A Cooper (additional)

References 0 Referenced 56

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 8:22 p.m.)
Deposited	2 years, 1 month ago (July 21, 2023, 10 p.m.)
Indexed	1 year, 1 month ago (July 11, 2024, 7:07 p.m.)
Issued	33 years, 6 months ago (Feb. 15, 1992)
Published	33 years, 6 months ago (Feb. 15, 1992)
Published Online	33 years, 6 months ago (Feb. 15, 1992)
Published Print	33 years, 6 months ago (Feb. 15, 1992)

Funders 0

None

BibTeX

@article{Hug_1992, title={Identification and characterization of an actin-binding site of CapZ.}, volume={116}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.116.4.923}, DOI={10.1083/jcb.116.4.923}, number={4}, journal={The Journal of cell biology}, publisher={Rockefeller University Press}, author={Hug, C and Miller, T M and Torres, M A and Casella, J F and Cooper, J A}, year={1992}, month=feb, pages={923–931} }

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