DOI: 10.1083/jcb.114.4.609. Characterization of YDJ1: a yeast homologue of the bacterial dnaJ protein.

Characterization of YDJ1: a yeast homologue of the bacterial dnaJ protein.

10.1083/jcb.114.4.609

Crossref journal-article

Rockefeller University Press

The Journal of cell biology (291)

Abstract

The YDJ1 (yeast dnaJ) gene was isolated from a yeast expression library using antisera made against a yeast nuclear sub-fraction termed the matrix lamina pore complex. The predicted open reading frame displays a 32% identity with the sequence of the Escherichia coli heat shock protein dnaJ. Localization of YDJ1 protein (YDJ1p) by indirect immunofluorescence reveals it to be concentrated in a perinuclear ring as well as in the cytoplasm. YDJ1p cofractionates with nuclei and also microsomes, suggesting that its perinuclear localization reflects association with the ER. YDJ1p is required for normal growth and disruption of its gene results in very slow growing cells that have pleiotropic morphological defects. Haploid cells carrying the disrupted YDJ1 gene are inviable for growth in liquid media. We further show that a related yeast protein, SIS1, is a multicopy suppressor of YDJ1.

Bibliography

Caplan, A. J., & Douglas, M. G. (1991). Characterization of YDJ1: a yeast homologue of the bacterial dnaJ protein. The Journal of Cell Biology, 114(4), 609â621.

Authors 2

A J Caplan (first)
M G Douglas (additional)

References 0 Referenced 225

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 8:18 p.m.)
Deposited	2 years, 1 month ago (July 21, 2023, 9:27 p.m.)
Indexed	2 weeks, 6 days ago (Aug. 5, 2025, 8:29 a.m.)
Issued	34 years ago (Aug. 15, 1991)
Published	34 years ago (Aug. 15, 1991)
Published Online	34 years ago (Aug. 15, 1991)
Published Print	34 years ago (Aug. 15, 1991)

Funders 0

None

BibTeX

@article{Caplan_1991, title={Characterization of YDJ1: a yeast homologue of the bacterial dnaJ protein.}, volume={114}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.114.4.609}, DOI={10.1083/jcb.114.4.609}, number={4}, journal={The Journal of cell biology}, publisher={Rockefeller University Press}, author={Caplan, A J and Douglas, M G}, year={1991}, month=aug, pages={609–621} }

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