DOI: 10.1083/jcb.112.4.615. Cholesterol is required for infection by Semliki Forest virus.

Cholesterol is required for infection by Semliki Forest virus.

10.1083/jcb.112.4.615

Crossref journal-article

Rockefeller University Press

The Journal of cell biology (291)

Abstract

Semliki Forest virus (SFV) and many other enveloped animal viruses enter cells by a membrane fusion reaction triggered by the low pH within the endocytic pathway. In vitro, SFV fusion requires cholesterol in the target membrane, but the role of cholesterol in vivo is unknown. In this paper, the infection pathway of SFV was studied in mammalian and inset cells substantially depleted of sterol. Cholesterol-depleted cells were unaltered in their ability to bind, internalize, and acidify virus, but were blocked in SFV fusion and subsequent virus replication. Depleted cells could be infected by the cholesterol-independent vesicular stomatitis virus, which also enters cells via endocytosis and low pH-mediated fusion. The block in SFV infection was specifically reversed by cholesterol but not by cholestenone, which lacks the critical 3 beta-hydroxyl group. Cholesterol thus is central in the infection pathway of SFV, and may act in vivo to modulate infection by SFV and other pathogens.

Bibliography

Phalen, T., & Kielian, M. (1991). Cholesterol is required for infection by Semliki Forest virus. The Journal of Cell Biology, 112(4), 615â623.

Authors 2

T Phalen (first)
M Kielian (additional)

References 0 Referenced 142

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 8:18 p.m.)
Deposited	2 years, 1 month ago (July 21, 2023, 9:18 p.m.)
Indexed	1 month ago (July 28, 2025, 5:58 p.m.)
Issued	34 years, 6 months ago (Feb. 15, 1991)
Published	34 years, 6 months ago (Feb. 15, 1991)
Published Online	34 years, 6 months ago (Feb. 15, 1991)
Published Print	34 years, 6 months ago (Feb. 15, 1991)

Funders 0

None

BibTeX

@article{Phalen_1991, title={Cholesterol is required for infection by Semliki Forest virus.}, volume={112}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.112.4.615}, DOI={10.1083/jcb.112.4.615}, number={4}, journal={The Journal of cell biology}, publisher={Rockefeller University Press}, author={Phalen, T and Kielian, M}, year={1991}, month=feb, pages={615–623} }

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