DOI: 10.1083/jcb.109.5.2145. A glycophospholipid membrane anchor acts as an apical targeting signal in polarized epithelial cells.

A glycophospholipid membrane anchor acts as an apical targeting signal in polarized epithelial cells.

10.1083/jcb.109.5.2145

Crossref journal-article

Rockefeller University Press

The Journal of cell biology (291)

Abstract

Glycosyl-phosphatidylinositol- (GPI) anchored proteins contain a large extracellular protein domain that is linked to the membrane via a glycosylated form of phosphatidylinositol. We recently reported the polarized apical distribution of all endogenous GPI-anchored proteins in the MDCK cell line (Lisanti, M. P., M. Sargiacomo, L. Graeve, A. R. Saltiel, and E. Rodriguez-Boulan. 1988. Proc. Natl. Acad. Sci. USA. 85:9557-9561). To study the role of this mechanism of membrane anchoring in targeting to the apical cell surface, we use here decay-accelerating factor (DAF) as a model GPI-anchored protein. Endogenous DAF was localized on the apical surface of two human intestinal cell lines (Caco-2 and SK-CO15). Recombinant DAF, expressed in MDCK cells, also assumed a polarized apical distribution. Transfer of the 37-amino acid DAF signal for GPI attachment to the ectodomain of herpes simplex glycoprotein D (a basolateral antigen) and to human growth hormone (a regulated secretory protein) by recombinant DNA methods resulted in delivery of the fusion proteins to the apical surface of transfected MDCK cells. These results are consistent with the notion that the GPI anchoring mechanism may convey apical targeting information.

Bibliography

Lisanti, M. P., Caras, I. W., Davitz, M. A., & Rodriguez-Boulan, E. (1989). A glycophospholipid membrane anchor acts as an apical targeting signal in polarized epithelial cells. The Journal of Cell Biology, 109(5), 2145â2156.

Authors 4

M P Lisanti (first)
I W Caras (additional)
M A Davitz (additional)
E Rodriguez-Boulan (additional)

References 0 Referenced 369

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 8:18 p.m.)
Deposited	2 years, 1 month ago (July 21, 2023, 8:50 p.m.)
Indexed	2 weeks ago (Aug. 7, 2025, 4:53 a.m.)
Issued	35 years, 9 months ago (Nov. 1, 1989)
Published	35 years, 9 months ago (Nov. 1, 1989)
Published Online	35 years, 9 months ago (Nov. 1, 1989)
Published Print	35 years, 9 months ago (Nov. 1, 1989)

Funders 0

None

BibTeX

@article{Lisanti_1989, title={A glycophospholipid membrane anchor acts as an apical targeting signal in polarized epithelial cells.}, volume={109}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.109.5.2145}, DOI={10.1083/jcb.109.5.2145}, number={5}, journal={The Journal of cell biology}, publisher={Rockefeller University Press}, author={Lisanti, M P and Caras, I W and Davitz, M A and Rodriguez-Boulan, E}, year={1989}, month=nov, pages={2145–2156} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        7
      ]
    ],
    "date-time": "2025-08-07T08:53:27Z",
    "timestamp": 1754556807671
  },
  "reference-count": 0,
  "publisher": "Rockefeller University Press",
  "issue": "5",
  "content-domain": {
    "domain": [
      "rupress.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1989,
        11,
        1
      ]
    ]
  },
  "abstract": "<jats:p>Glycosyl-phosphatidylinositol- (GPI) anchored proteins contain a large extracellular protein domain that is linked to the membrane via a glycosylated form of phosphatidylinositol. We recently reported the polarized apical distribution of all endogenous GPI-anchored proteins in the MDCK cell line (Lisanti, M. P., M. Sargiacomo, L. Graeve, A. R. Saltiel, and E. Rodriguez-Boulan. 1988. Proc. Natl. Acad. Sci. USA. 85:9557-9561). To study the role of this mechanism of membrane anchoring in targeting to the apical cell surface, we use here decay-accelerating factor (DAF) as a model GPI-anchored protein. Endogenous DAF was localized on the apical surface of two human intestinal cell lines (Caco-2 and SK-CO15). Recombinant DAF, expressed in MDCK cells, also assumed a polarized apical distribution. Transfer of the 37-amino acid DAF signal for GPI attachment to the ectodomain of herpes simplex glycoprotein D (a basolateral antigen) and to human growth hormone (a regulated secretory protein) by recombinant DNA methods resulted in delivery of the fusion proteins to the apical surface of transfected MDCK cells. These results are consistent with the notion that the GPI anchoring mechanism may convey apical targeting information.</jats:p>",
  "DOI": "10.1083/jcb.109.5.2145",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2004,
        5,
        15
      ]
    ],
    "date-time": "2004-05-15T00:18:20Z",
    "timestamp": 1084580300000
  },
  "page": "2145-2156",
  "update-policy": "http://dx.doi.org/10.1083/jcb.crossmarkpolicy",
  "source": "Crossref",
  "is-referenced-by-count": 369,
  "title": "A glycophospholipid membrane anchor acts as an apical targeting signal in polarized epithelial cells.",
  "prefix": "10.1083",
  "volume": "109",
  "author": [
    {
      "given": "M P",
      "family": "Lisanti",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Department of Cell Biology and Anatomy, Cornell University Medical College, New York 10021."
        }
      ]
    },
    {
      "given": "I W",
      "family": "Caras",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Cell Biology and Anatomy, Cornell University Medical College, New York 10021."
        }
      ]
    },
    {
      "given": "M A",
      "family": "Davitz",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Cell Biology and Anatomy, Cornell University Medical College, New York 10021."
        }
      ]
    },
    {
      "given": "E",
      "family": "Rodriguez-Boulan",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Cell Biology and Anatomy, Cornell University Medical College, New York 10021."
        }
      ]
    }
  ],
  "member": "291",
  "published-online": {
    "date-parts": [
      [
        1989,
        11,
        1
      ]
    ]
  },
  "container-title": "The Journal of cell biology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://rupress.org/jcb/article-pdf/109/5/2145/1462458/2145.pdf",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "syndication"
    },
    {
      "URL": "https://rupress.org/jcb/article-pdf/109/5/2145/1462458/2145.pdf",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        7,
        22
      ]
    ],
    "date-time": "2023-07-22T00:50:54Z",
    "timestamp": 1689987054000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://rupress.org/jcb/article/109/5/2145/55769/A-glycophospholipid-membrane-anchor-acts-as-an"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1989,
        11,
        1
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "5",
    "published-print": {
      "date-parts": [
        [
          1989,
          11,
          1
        ]
      ]
    }
  },
  "URL": "http://dx.doi.org/10.1083/jcb.109.5.2145",
  "relation": {},
  "ISSN": [
    "0021-9525",
    "1540-8140"
  ],
  "subject": [],
  "published": {
    "date-parts": [
      [
        1989,
        11,
        1
      ]
    ]
  }
}