DOI: 10.1083/jcb.109.5.1975. High-mobility group protein HMG-I localizes to G/Q- and C-bands of human and mouse chromosomes.

High-mobility group protein HMG-I localizes to G/Q- and C-bands of human and mouse chromosomes.

10.1083/jcb.109.5.1975

Crossref journal-article

Rockefeller University Press

The Journal of cell biology (291)

Abstract

Mammalian metaphase chromosomes can be identified by their characteristic banding pattern when stained with Giemsa dye after brief proteolytic digestion. The resulting G-bands are known to contain regions of DNA enriched in A/T residues and to be the principal location for the L1 (or Kpn 1) family of long interspersed repetitive sequences in human chromosomes. Here we report that antibodies raised against a highly purified and biochemically well characterized nonhistone "High-Mobility Group" protein, HMG-I, specifically localize this protein to the G-bands in mammalian metaphase chromosomes. In some preparations in which chromosomes are highly condensed, HMG-I appears to be located at the centromere and/or telomere regions of mammalian chromosomes as well. To our knowledge, this is the first well-characterized mammalian protein that localizes primarily to G-band regions of chromosomes.

Bibliography

Disney, J. E., Johnson, K. R., Magnuson, N. S., Sylvester, S. R., & Reeves, R. (1989). High-mobility group protein HMG-I localizes to G/Q- and C-bands of human and mouse chromosomes. The Journal of Cell Biology, 109(5), 1975â1982.

Authors 5

J E Disney (first)
K R Johnson (additional)
N S Magnuson (additional)
S R Sylvester (additional)
R Reeves (additional)

References 0 Referenced 98

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 8:18 p.m.)
Deposited	2 years, 1 month ago (July 21, 2023, 9:46 p.m.)
Indexed	3 weeks ago (Aug. 5, 2025, 8:59 a.m.)
Issued	35 years, 9 months ago (Nov. 1, 1989)
Published	35 years, 9 months ago (Nov. 1, 1989)
Published Online	35 years, 9 months ago (Nov. 1, 1989)
Published Print	35 years, 9 months ago (Nov. 1, 1989)

Funders 0

None

BibTeX

@article{Disney_1989, title={High-mobility group protein HMG-I localizes to G/Q- and C-bands of human and mouse chromosomes.}, volume={109}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.109.5.1975}, DOI={10.1083/jcb.109.5.1975}, number={5}, journal={The Journal of cell biology}, publisher={Rockefeller University Press}, author={Disney, J E and Johnson, K R and Magnuson, N S and Sylvester, S R and Reeves, R}, year={1989}, month=nov, pages={1975–1982} }

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