DOI: 10.1083/jcb.109.3.983. Yeast proteins that recognize nuclear localization sequences.

Yeast proteins that recognize nuclear localization sequences.

10.1083/jcb.109.3.983

Crossref journal-article

Rockefeller University Press

The Journal of cell biology (291)

Abstract

A variety of peptides can mediate the localization of proteins to the nucleus. We have identified yeast proteins of 70 and 59 kD that bind to nuclear localization peptides of SV-40 T antigen, Xenopus nucleoplasmin, and the yeast proteins Ga14 and histone H2B. These proteins are assayed by the binding of peptide-albumin conjugates to proteins immobilized on nitrocellulose filters. These binding proteins fractionate with nuclei and are extractable with salt but not detergent. Radiolabeled peptide-albumin conjugates also bind to isolated nuclei; the binding is saturable and can be extracted with salt. Different nuclear localization peptides compete with each other, implying that a single class of proteins is responsible for their recognition. The 70- and 59-kD proteins have the properties expected for a receptor that would act to direct proteins to the nucleus.

Bibliography

Silver, P., Sadler, I., & Osborne, M. A. (1989). Yeast proteins that recognize nuclear localization sequences. The Journal of Cell Biology, 109(3), 983â989.

Authors 3

P Silver (first)
I Sadler (additional)
M A Osborne (additional)

References 0 Referenced 65

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 8:18 p.m.)
Deposited	2 years, 1 month ago (July 21, 2023, 9:05 p.m.)
Indexed	1 month, 3 weeks ago (July 2, 2025, 2:45 p.m.)
Issued	35 years, 11 months ago (Sept. 1, 1989)
Published	35 years, 11 months ago (Sept. 1, 1989)
Published Online	35 years, 11 months ago (Sept. 1, 1989)
Published Print	35 years, 11 months ago (Sept. 1, 1989)

Funders 0

None

BibTeX

@article{Silver_1989, title={Yeast proteins that recognize nuclear localization sequences.}, volume={109}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.109.3.983}, DOI={10.1083/jcb.109.3.983}, number={3}, journal={The Journal of cell biology}, publisher={Rockefeller University Press}, author={Silver, P and Sadler, I and Osborne, M A}, year={1989}, month=sep, pages={983–989} }

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