DOI: 10.1083/jcb.109.3.1015. The GTP-binding protein Ypt1 is required for transport in vitro: the Golgi apparatus is defective in ypt1 mutants.

The GTP-binding protein Ypt1 is required for transport in vitro: the Golgi apparatus is defective in ypt1 mutants.

10.1083/jcb.109.3.1015

Crossref journal-article

Rockefeller University Press

The Journal of cell biology (291)

Abstract

The YPT1 gene encodes a raslike, GTP-binding protein that is essential for growth of yeast cells. We show here that mutations in the ypt1 gene disrupt transport of carboxypeptidase Y to the vacuole in vivo and transport of pro-alpha-factor to a site of extensive glycosylation in the Golgi apparatus in vitro. Two different ypt1 mutations result in loss of function of the Golgi complex without affecting the activity of the endoplasmic reticulum or soluble components required for in vitro transport. The function of the mutant Golgi apparatus can be restored by preincubation with wild-type cytosol. The transport defect observed in vitro cannot be overcome by addition of Ca++ to the reaction mixture. We have also established genetic interactions between ypt1 and a subset of the other genes required for transport to and through the Golgi apparatus.

Bibliography

Bacon, R. A., Salminen, A., Ruohola, H., Novick, P., & Ferro-Novick, S. (1989). The GTP-binding protein Ypt1 is required for transport in vitro: the Golgi apparatus is defective in ypt1 mutants. The Journal of Cell Biology, 109(3), 1015â1022.

Authors 5

R A Bacon (first)
A Salminen (additional)
H Ruohola (additional)
P Novick (additional)
S Ferro-Novick (additional)

References 0 Referenced 159

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 8:18 p.m.)
Deposited	2 years, 1 month ago (July 21, 2023, 9:01 p.m.)
Indexed	3 weeks, 6 days ago (Aug. 7, 2025, 4:33 p.m.)
Issued	36 years ago (Sept. 1, 1989)
Published	36 years ago (Sept. 1, 1989)
Published Online	36 years ago (Sept. 1, 1989)
Published Print	36 years ago (Sept. 1, 1989)

Funders 0

None

BibTeX

@article{Bacon_1989, title={The GTP-binding protein Ypt1 is required for transport in vitro: the Golgi apparatus is defective in ypt1 mutants.}, volume={109}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.109.3.1015}, DOI={10.1083/jcb.109.3.1015}, number={3}, journal={The Journal of cell biology}, publisher={Rockefeller University Press}, author={Bacon, R A and Salminen, A and Ruohola, H and Novick, P and Ferro-Novick, S}, year={1989}, month=sep, pages={1015–1022} }

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