DOI: 10.1083/jcb.108.4.1387. Analysis of the signal for attachment of a glycophospholipid membrane anchor.

Analysis of the signal for attachment of a glycophospholipid membrane anchor.

10.1083/jcb.108.4.1387

Crossref journal-article

Rockefeller University Press

The Journal of cell biology (291)

Abstract

The COOH terminus of decay accelerating factor (DAF) contains a signal that directs attachment of a glycophospholipid (GPI) membrane anchor. To define this signal we deleted portions of the DAF COOH terminus and expressed the mutant cDNAs it CV1 origin-deficient SV-40 cells. Our results show that the COOH-terminal hydrophobic domain (17 residues) is absolutely required for GPI anchor attachment. However, when fused to the COOH terminus of a secreted protein this hydrophobic domain is insufficient to direct attachment of a GPI anchor. Additional specific information located within the adjacent 20 residues appears to be necessary. We speculate that by analogy with signal sequences for membrane translocation, GPI anchor attachment requires both a COOH-terminal hydrophobic domain (the GPI signal) as well as a suitable cleavage/attachment site located NH2 terminal to the signal.

Bibliography

Caras, I. W., Weddell, G. N., & Williams, S. R. (1989). Analysis of the signal for attachment of a glycophospholipid membrane anchor. The Journal of Cell Biology, 108(4), 1387â1396.

Authors 3

I W Caras (first)
G N Weddell (additional)
S R Williams (additional)

References 0 Referenced 95

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 8:18 p.m.)
Deposited	2 years, 1 month ago (July 21, 2023, 8:50 p.m.)
Indexed	1 month, 2 weeks ago (July 14, 2025, 11:14 p.m.)
Issued	36 years, 5 months ago (April 1, 1989)
Published	36 years, 5 months ago (April 1, 1989)
Published Online	36 years, 5 months ago (April 1, 1989)
Published Print	36 years, 5 months ago (April 1, 1989)

Funders 0

None

BibTeX

@article{Caras_1989, title={Analysis of the signal for attachment of a glycophospholipid membrane anchor.}, volume={108}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.108.4.1387}, DOI={10.1083/jcb.108.4.1387}, number={4}, journal={The Journal of cell biology}, publisher={Rockefeller University Press}, author={Caras, I W and Weddell, G N and Williams, S R}, year={1989}, month=apr, pages={1387–1396} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        7,
        15
      ]
    ],
    "date-time": "2025-07-15T03:14:23Z",
    "timestamp": 1752549263967
  },
  "reference-count": 0,
  "publisher": "Rockefeller University Press",
  "issue": "4",
  "content-domain": {
    "domain": [
      "rupress.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1989,
        4,
        1
      ]
    ]
  },
  "abstract": "<jats:p>The COOH terminus of decay accelerating factor (DAF) contains a signal that directs attachment of a glycophospholipid (GPI) membrane anchor. To define this signal we deleted portions of the DAF COOH terminus and expressed the mutant cDNAs it CV1 origin-deficient SV-40 cells. Our results show that the COOH-terminal hydrophobic domain (17 residues) is absolutely required for GPI anchor attachment. However, when fused to the COOH terminus of a secreted protein this hydrophobic domain is insufficient to direct attachment of a GPI anchor. Additional specific information located within the adjacent 20 residues appears to be necessary. We speculate that by analogy with signal sequences for membrane translocation, GPI anchor attachment requires both a COOH-terminal hydrophobic domain (the GPI signal) as well as a suitable cleavage/attachment site located NH2 terminal to the signal.</jats:p>",
  "DOI": "10.1083/jcb.108.4.1387",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2004,
        5,
        15
      ]
    ],
    "date-time": "2004-05-15T00:18:20Z",
    "timestamp": 1084580300000
  },
  "page": "1387-1396",
  "update-policy": "http://dx.doi.org/10.1083/jcb.crossmarkpolicy",
  "source": "Crossref",
  "is-referenced-by-count": 95,
  "title": "Analysis of the signal for attachment of a glycophospholipid membrane anchor.",
  "prefix": "10.1083",
  "volume": "108",
  "author": [
    {
      "given": "I W",
      "family": "Caras",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Genentech, Inc., South San Francisco, California 94080."
        }
      ]
    },
    {
      "given": "G N",
      "family": "Weddell",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Genentech, Inc., South San Francisco, California 94080."
        }
      ]
    },
    {
      "given": "S R",
      "family": "Williams",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Genentech, Inc., South San Francisco, California 94080."
        }
      ]
    }
  ],
  "member": "291",
  "published-online": {
    "date-parts": [
      [
        1989,
        4,
        1
      ]
    ]
  },
  "container-title": "The Journal of cell biology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://rupress.org/jcb/article-pdf/108/4/1387/1462388/1387.pdf",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "syndication"
    },
    {
      "URL": "https://rupress.org/jcb/article-pdf/108/4/1387/1462388/1387.pdf",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        7,
        22
      ]
    ],
    "date-time": "2023-07-22T00:50:21Z",
    "timestamp": 1689987021000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://rupress.org/jcb/article/108/4/1387/58997/Analysis-of-the-signal-for-attachment-of-a"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1989,
        4,
        1
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "4",
    "published-print": {
      "date-parts": [
        [
          1989,
          4,
          1
        ]
      ]
    }
  },
  "URL": "http://dx.doi.org/10.1083/jcb.108.4.1387",
  "relation": {},
  "ISSN": [
    "0021-9525",
    "1540-8140"
  ],
  "subject": [],
  "published": {
    "date-parts": [
      [
        1989,
        4,
        1
      ]
    ]
  }
}