DOI: 10.1083/jcb.106.3.991. D-periodic distribution of collagen type IX along cartilage fibrils.

D-periodic distribution of collagen type IX along cartilage fibrils.

10.1083/jcb.106.3.991

Crossref journal-article

Rockefeller University Press

The Journal of cell biology (291)

Abstract

It has recently become apparent that collagen fibrils may be composed of more than one kind of macromolecule. To explore this possibility, we developed a procedure to purify fibril fragments from 17-d embryonic chicken sternal cartilage. The fibril population obtained shows, after negative staining, a uniformity in the banding pattern and diameter similar to the fibrils in situ. Pepsin digestion of this fibril preparation releases collagen types II, IX, and XI in the proportion of 8:1:1. Rotary shadowing of the fibrils reveals a d-periodic distribution of 35-40-nm long projections, each capped with a globular domain, which resemble in form and dimensions the aminoterminal globular and collagenous domains, NC4 and COL3, of type IX collagen. The monoclonal antibody (4D6) specific for an epitope close to the amino terminal of the COL3 domain of type IX collagen bound to these projections, thus confirming their identity. Type IX collagen is therefore distributed in a regular d-periodic arrangement along cartilage fibrils, with the chondroitin sulfate chain of type IX collagen in intimate contact with the fibril.

Bibliography

Vaughan, L., Mendler, M., Huber, S., Bruckner, P., Winterhalter, K. H., Irwin, M. I., & Mayne, R. (1988). D-periodic distribution of collagen type IX along cartilage fibrils. The Journal of Cell Biology, 106(3), 991â997.

Authors 7

L Vaughan (first)
M Mendler (additional)
S Huber (additional)
P Bruckner (additional)
K H Winterhalter (additional)
M I Irwin (additional)
R Mayne (additional)

References 0 Referenced 276

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 8:18 p.m.)
Deposited	2 years, 1 month ago (July 21, 2023, 9:32 p.m.)
Indexed	2 months, 2 weeks ago (June 13, 2025, 12:25 a.m.)
Issued	37 years, 5 months ago (March 1, 1988)
Published	37 years, 5 months ago (March 1, 1988)
Published Online	37 years, 5 months ago (March 1, 1988)
Published Print	37 years, 5 months ago (March 1, 1988)

Funders 0

None

BibTeX

@article{Vaughan_1988, title={D-periodic distribution of collagen type IX along cartilage fibrils.}, volume={106}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.106.3.991}, DOI={10.1083/jcb.106.3.991}, number={3}, journal={The Journal of cell biology}, publisher={Rockefeller University Press}, author={Vaughan, L and Mendler, M and Huber, S and Bruckner, P and Winterhalter, K H and Irwin, M I and Mayne, R}, year={1988}, month=mar, pages={991–997} }

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