DOI: 10.1083/jcb.106.1.127. Structure and mass analysis of 14S dynein obtained from Tetrahymena cilia.

Structure and mass analysis of 14S dynein obtained from Tetrahymena cilia.

10.1083/jcb.106.1.127

Crossref journal-article

Rockefeller University Press

The Journal of cell biology (291)

Abstract

Scanning transmission electron microscopic analysis revealed that the 14S fraction of Tetrahymena dynein was of a mixture of two types of particles in approximately equal proportions. The 14S dynein molecules were roughly ellipsoid in shape with approximate axes of 9.5 and 14.5 nm. About half of the particles had tails 20-24-nm long. By the integration of electron scattering intensities, particles with tails had an average mass of 510 kD with a SD of 90 kD. The globular heads of both types of particles had an average mass of 330 kD with a SD of 60 kD. The mass of the tail structure was about 180 kD. By SDS-PAGE, the 14S dynein consisted of two high molecular mass polypeptides above 300 kD that could be distinguished by immunoblot analysis.

Bibliography

Marchese-Ragona, S. P., Wall, J. S., & Johnson, K. A. (1988). Structure and mass analysis of 14S dynein obtained from Tetrahymena cilia. The Journal of Cell Biology, 106(1), 127â132.

Authors 3

S P Marchese-Ragona (first)
J S Wall (additional)
K A Johnson (additional)

References 0 Referenced 34

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 8:18 p.m.)
Deposited	2 years, 1 month ago (July 21, 2023, 8:24 p.m.)
Indexed	1 month, 3 weeks ago (July 8, 2025, 7:06 a.m.)
Issued	37 years, 7 months ago (Jan. 1, 1988)
Published	37 years, 7 months ago (Jan. 1, 1988)
Published Online	37 years, 7 months ago (Jan. 1, 1988)
Published Print	37 years, 7 months ago (Jan. 1, 1988)

Funders 0

None

BibTeX

@article{Marchese_Ragona_1988, title={Structure and mass analysis of 14S dynein obtained from Tetrahymena cilia.}, volume={106}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.106.1.127}, DOI={10.1083/jcb.106.1.127}, number={1}, journal={The Journal of cell biology}, publisher={Rockefeller University Press}, author={Marchese-Ragona, S P and Wall, J S and Johnson, K A}, year={1988}, month=jan, pages={127–132} }

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