DOI: 10.1083/jcb.104.5.1249. Modulation of the expression of an apical plasma membrane protein of Madin-Darby canine kidney epithelial cells: cell-cell interactions control the appearance of a novel intracellular storage compartment.

Modulation of the expression of an apical plasma membrane protein of Madin-Darby canine kidney epithelial cells: cell-cell interactions control the appearance of a novel intracellular storage compartment.

10.1083/jcb.104.5.1249

Crossref journal-article

Rockefeller University Press

The Journal of cell biology (291)

Abstract

Experimental conditions that abolish or reduce to a minimum intercellular contacts between Madin-Darby canine kidney epithelial cells result in the appearance of an intracellular storage compartment for apical membrane proteins. Subconfluent culture, incubation in 1-5 microM Ca++, or inclusion of dissociated cells within agarose or collagen gels all caused the intracellular accumulation of a 184-kD apical membrane protein within large (0.5-5 micron) vacuoles, rich in microvilli. Influenza virus hemagglutinin, an apically targeted viral glycoprotein, is concentrated within these structures but the basolateral glycoprotein G of vesicular stomatitis virus and a cellular basolateral 63-kD membrane protein of Madin-Darby canine kidney cells were excluded. This novel epithelial organelle (VAC), which we designate the vacuolar apical compartment, may play an as yet unrecognized role in the biogenesis of the apical plasma membrane during the differentiation of normal epithelia.

Bibliography

Vega-Salas, D. E., Salas, P. J., & Rodriguez-Boulan, E. (1987). Modulation of the expression of an apical plasma membrane protein of Madin-Darby canine kidney epithelial cells: cell-cell interactions control the appearance of a novel intracellular storage compartment. The Journal of Cell Biology, 104(5), 1249â1259.

Authors 3

D E Vega-Salas (first)
P J Salas (additional)
E Rodriguez-Boulan (additional)

References 0 Referenced 111

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 8:18 p.m.)
Deposited	2 years, 1 month ago (July 21, 2023, 8:13 p.m.)
Indexed	1 week ago (Aug. 23, 2025, 1 a.m.)
Issued	38 years, 3 months ago (May 1, 1987)
Published	38 years, 3 months ago (May 1, 1987)
Published Online	38 years, 3 months ago (May 1, 1987)
Published Print	38 years, 3 months ago (May 1, 1987)

Funders 0

None

BibTeX

@article{Vega_Salas_1987, title={Modulation of the expression of an apical plasma membrane protein of Madin-Darby canine kidney epithelial cells: cell-cell interactions control the appearance of a novel intracellular storage compartment.}, volume={104}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.104.5.1249}, DOI={10.1083/jcb.104.5.1249}, number={5}, journal={The Journal of cell biology}, publisher={Rockefeller University Press}, author={Vega-Salas, D E and Salas, P J and Rodriguez-Boulan, E}, year={1987}, month=may, pages={1249–1259} }

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